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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20以上
- 供应商:
深圳市康初源有限公司
- 规格:
1×10⁶cells/T25培养瓶
|
种属 |
人 |
|
别称 |
DMS-114; DMS114; Darmouth Medical School 114 |
|
组织来源 |
肺 |
|
疾病 |
肺癌组织 |
|
传代比例/细胞消化 |
1:2传代,消化2-3分钟 |
|
完全培养基配置 |
RPMI1640培养基;10%胎牛血清;1%双抗 |
|
简介 |
患者之前没有接受过治疗。该细胞表达 HLA I 类和 II 类抗原。细胞早期传代时被牛支原体(莱氏无胆甾原体)污染,在冻存前使用牛莱氏无胆甾原体抗血清(A. laidlawii antiserum)和卡那霉素治愈。 |
|
形态 |
上皮细胞样 |
|
生长特征 |
贴壁生长 |
|
倍增时间 |
每周 2 至 3 次 |
|
抗原表达 |
Leu 7 +; My23 +; CD11b + |
|
基因标本 |
adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) |
|
致瘤性 |
Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
|
STR |
Amelogenin: X,X;CSF1PO: 10,11;D13S317: 13,13;D16S539: 12,12;D5S818: 12,12;D7S820: 10,11;THO1: 8,9.3;TPOX: 8,11;vWA: 16,17. |
|
培养条件 |
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液:90%FBS,DMSO 10%, 或使用非程序冻存液:官网货号JY-H040 |
|
保藏机构 |
ATCC; CRL-2066 |
|
产品使用 |
仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。 |
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