1574-A赛默飞1500系列A2型生物安全柜，1.2m，不

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

Western Blotting

-S in 500 ml 1% acetic acid(Fisher# BP103-50)Developing Solution100 mM Tris 9.5 15 ml 1M Tris 9.5100 mM NaCl 3 ml 5M NaCl5 mM MgCl2 0.75 ml 1M MgCl2up to 150ml with QTo use, add 66 ml NBT and 50 ml BCIP to 15 ml of Developing Solution.NBT (sigma N

Small scale DNA preps for Neurospora crassa.

to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex