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文献和实验A Peptide Microarray for Detecting Protein Kinase Activity in Cell Lysates
on a glutaraldehyde-premodified high-amino terminal glass slide, by spotting 2 nL volumes of substrate peptide solutions with an automated microarray spotter. After the peptides are phosphorylated by cell lysates, phosphorylation is specifically recognized
Measurement of Wee Kinase Activity
or in purified preparations. Specific protocols include the production and purification of recombinant Cdc2/Cyclin B substrate, the production of crude subcellular extract fractions, the purification of endogenous or recombinant Wee kinases, Wee kinase assays
Assaying Protein Kinase Activity
for heterologous expression of the kinase in yeast Saccharomyces cerevisiae , immunoaffinity purification from yeast cell lysates, kinase reactions using incorporation of 32 P into peptide substrates, and quantifying protein kinase activity. The Notes section
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