- 询价
- Nebulabio
- 中国
- NBL-292612
- 2026年01月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
见COA
- 库存：
大量
- 供应商：
北京辰辉创聚生物技术有限公司
- 规格：
100ug; 500ug; 1mg; Bulk
| 产品信息
品名：NebuSelect™ Recombinant Vibrio vulnificus vvhA/Cytolysin Protein, 21-471aa, His-tag, SUMO-tag
中文名称：重组Vibrio vulnificus vvhA/Cytolysin 蛋白
别名：Cytolysin, vvhA, VV2_0404
货号：NBL-292612
品牌：Nebulabio
规格：100UG；1MG；Bulk
| 产品描述
NebuSelect™ Recombinant Vibrio vulnificus vvhA/Cytolysin Protein, His-tag, SUMO-tag (Cat# NBL-292612) is expressed in E. coli. This recombinant protein is of high purity, verified to be greater than 90% by SDS-PAGE. Endotoxin levels are less than 1 EU/µg, as determined by the LAL (Limulus Amebocyte Lysate) assay.
| 产品属性
Species:Vibrio vulnificus
Expression System:E. coli
Molecular Weight:63 kDa
Target Name:vvhA/Cytolysin
Purity:>90% by SDS-PAGE.
Formulation:See COA
Endotoxin:＜1 EU/ug(LAL Method)
Storage:4°C for short term. -20°C for long term.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验Strategies to Optimize Protein Expression in E. coli
., Su, X.Z., and Rawlings, C.A. 1995. Expression of recombinant feline tumor‐necrosis‐factor is toxic to Escherichia coli. Clin. Diagn. Lab. Immunol. 2:740‐746. Park, S.L
bodies, Biopolymers (peptide science) 51 (1999) 297-307. 4. E.B. Clark, Refolding of recombinant proteins, Curr. Opin. Biotechnol. 9 (1998) 157-163. 5. E.B. Clark, Protein refolding for industrial processes, Curr. Opin. Biotechnol. 12 (2001) 202-207
方案。纯化的方法我们有层析、沉淀、萃取、透析、超滤等等。 柱层析是纯化最强有力的手段，几乎每种蛋白的纯化都会用到层析。各种层析方法：如亲和、离子交换、疏水、反相、分子筛等等都是我们工艺宝库里的吃饭家伙。不知道怎么吃啊，没办法了，你还是先去补一补基础知识吧。GE公司的一套书个人认为是最佳的入门资料，如Recombinant Protein Purification，Affinity Chromatography，Ion-Exchange chromatography，Gel Filtration
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