VEGF ELISA KIT 血管内皮生长因子ELISA试剂

EliKine™ 小鼠血管内皮生长因子(VEGF) ELISA试剂盒

产品货号：KTE7016-2

中文名称：VEGF ELISA KIT 血管内皮生长因子ELISA试剂盒(小鼠)

英文名称：Mouse VEGF ELISA Kit

产品概述

本试剂盒采用双抗体夹心酶联免疫吸附法(ELISA)，专用于定量检测小鼠来源的样本（如细胞培养上清、血清、血浆及其他生物液体）中的血管内皮生长因子(VEGF)蛋白浓度。作为细胞分析领域的核心产品，该试剂盒在血管生物学、肿瘤学及代谢疾病等研究领域具有重要的应用价值。

核心特性与优势

• 高灵敏度：最低检测浓度可达8 pg/mL，能够精准检测低丰度VEGF表达。
• 优异特异性：经过严格验证，对目标蛋白的类似物无明显交叉反应或背景干扰。
• 宽线性范围：检测范围为15.63 pg/mL - 1000 pg/mL，满足不同浓度样本的检测需求。
• 稳定可靠：采用预包被微孔板设计，配合优化的缓冲体系，确保实验结果的重现性。
• 便捷高效：试剂盒组分齐全，操作流程清晰，节省实验准备时间。

产品背景

血管内皮生长因子(VEGF)，亦称血管通透性因子(VPF)，是一种分子量约为34-45 kDa的同源二聚体糖蛋白。它对血管内皮细胞具有高度特异性，在促进血管通透性、内皮细胞增殖及新生血管形成等生理和病理过程中发挥核心作用。VEGF的表达与肿瘤转移、生殖调控以及糖尿病并发症（如肾病、视网膜病变）密切相关。小鼠VEGF (UniProt ID: Q00731) 是研究血管生成相关机制的重要模型蛋白。

试剂盒组分

• 小鼠源VEGF-A抗体预包被微孔板
• 小鼠源VEGF-A蛋白标准品
• 小鼠源VEGF-A检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液
• 实验缓冲液
• HRP底物
• 反应终止液
• 洗涤缓冲液（浓缩液）
• 封板膜

规格与定价

规格	价格 (元)	货期
48 T	1598.0	现货（次日发货）
96 T	2558.0	现货（次日发货）

保存与运输

保存建议：未开封试剂盒请于2-8℃保存；启用后，各组分请参照说明书要求分别储存。

运输条件：全程采用冰袋（蓝冰）低温运输，确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或超出有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 实验操作需使用一次性吸头，避免交叉污染。
• 未使用的板条应立即放回原包装并密封，4℃保存。
• 建议对样品和标准品进行复孔检测（双孔或三孔），以提高数据准确性。
• 实验过程中请充分混匀试剂，推荐使用低速振荡器或定期手动摇匀。

警告：本产品仅供科学研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可规定。

别名参考：VEGFA; VEGF-A; VPF; vascular permeability factor; 血管内皮生长因子ELISA试剂盒; 小鼠血管内皮生长因子ELISA试剂盒

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Mouse Vascular Endothelial Growth Factor (VEGF) ELISA Kit

Product Catalog No.: KTE7016-2

Chinese Name: VEGF ELISA KIT Vascular Endothelial Growth Factor ELISA Kit (Mouse)

English Name: Mouse VEGF ELISA Kit

Product Overview

This kit employs a sandwich enzyme‑linked immunosorbent assay (ELISA) designed specifically for the quantitative measurement of vascular endothelial growth factor (VEGF) protein concentrations in mouse‑derived samples (e.g., cell culture supernatants, serum, plasma, and other biological fluids). As a core product for cellular analysis, the kit provides valuable utility in vascular biology, oncology, and metabolic disease research.

Key Features and Advantages

• High Sensitivity: Minimum detection limit as low as 8 pg/mL, enabling precise quantification of low‑abundance VEGF expression.
• Outstanding Specificity: Rigorously validated with negligible cross‑reactivity or background interference from related proteins.
• Wide Linear Range: Quantifiable range of 15.63 pg/mL–1000 pg/mL, accommodating diverse sample concentrations.
• Stable and Reliable: Pre‑coated microplate format combined with optimized buffer system ensures reproducible results.
• Convenient and Efficient: Complete reagent set with a clear workflow reduces preparation time.

Product Background

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a homologous disulfide‑linked dimeric glycoprotein with a molecular weight of approximately 34–45 kDa. It exhibits high specificity for endothelial cells and plays a central role in physiological and pathological processes such as vascular permeability, endothelial cell proliferation, and angiogenesis. VEGF expression is closely associated with tumor metastasis, reproductive regulation, and diabetic complications (e.g., nephropathy, retinopathy). Mouse VEGF (UniProt ID: Q00731) serves as a pivotal model protein for elucidating angiogenic mechanisms.

Kit Components

• Pre‑coated microplate with mouse VEGF‑A antibody
• Mouse VEGF‑A recombinant standard
• Mouse VEGF‑A detection antibody
• EliKine™ Streptavidin‑HRP
• Standard dilution buffer
• Assay buffer
• HRP substrate
• Reaction stop solution
• Concentrated wash buffer
• Plate sealing film

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T	1598.0	In stock (next‑day shipment)
96 T	2558.0	In stock (next‑day shipment)

Storage and Shipping

Storage Recommendation: Store unopened kits at 2–8 °C; after opening, each component should be stored according to the instructions.

Shipping Conditions: Entire shipment is conducted on ice packs (blue ice) under refrigerated conditions to preserve reagent activity.

Important Precautions

• Do not mix reagents from different lot numbers or use reagents past their expiration date.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Unused plate strips should be promptly returned to their original packaging, sealed, and stored at 4 °C.
• It is recommended to run samples and standards in duplicate or triplicate to improve data accuracy.
• Mix reagents thoroughly during the assay; low‑speed orbital shaking or periodic manual mixing is advisable.

Warning: This product is for scientific research use only and is not intended for human or clinical diagnostic applications. Users must ensure that its use does not infringe any patents or licensing agreements.

Alias Reference: VEGFA; VEGF‑A; VPF; vascular permeability factor; Vascular Endothelial Growth Factor ELISA Kit; Mouse Vascular Endothelial Growth Factor ELISA Kit

Note: The above information may change due to product optimization or upgrades. Please contact EliKine™ for the most current details.