NOS2/NOS2A/INOS/HEP-NOS/I-NOS

产品概述

KTE4024-2 EliKine™ 小鼠诱导型NO合酶(iNOS) ELISA试剂盒是一款基于成熟的双抗体夹心法原理开发的高品质检测工具，专为精准定量小鼠血清、血浆、组织匀浆及细胞培养上清液等样本中的iNOS蛋白浓度而设计。本试剂盒隶属于细胞分析产品中心的核心产品线，严格遵循ELISA应用标准，为炎症、免疫应答及心血管疾病等相关领域的科学研究提供可靠的数据支持。

核心特性与优势

• 高灵敏度与宽线性范围：最低检测浓度可达8 pg/mL，检测范围覆盖40 pg/mL - 3200 pg/mL，确保在宽广的浓度区间内获得准确的定量结果。
• 卓越的特异性：经过严格验证，对目标蛋白iNOS具有高度特异性，与类似物无明显交叉反应，有效降低背景干扰，保障实验数据的可靠性。
• 便捷的一步法操作：优化的实验流程（一步法）简化了操作步骤，有助于提升实验效率，并减少因多步操作带来的潜在误差。
• 即用型完整组分：试剂盒提供包括预包被微孔板、标准品、检测抗体、酶结合物、显色底物及洗涤缓冲液在内的所有必要组分，开盒即用，方便快捷。

技术参数

试剂盒组分（48T/96T）

• Mouse iNOS 预包被微孔板
• Mouse iNOS 标准品 (6,400 pg/mL)
• 标准品稀释液
• Mouse iNOS 检测抗体（生物素化）
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP 显色底物 A 液 & B 液 (TMB)
• 终止液
• 洗涤缓冲液 (浓缩)
• 板盖

（注：具体组分含量可能因规格不同而异，请以实际收到的试剂盒说明书为准。）

背景信息

诱导型一氧化氮合酶 (iNOS，由NOS2基因编码) 是催化L-精氨酸产生一氧化氮 (NO) 的关键酶之一，主要在巨噬细胞等免疫细胞中受炎症因子诱导表达，在宿主防御、炎症反应及细胞信号转导中扮演重要角色。精准检测iNOS的表达水平对于深入研究相关病理生理过程至关重要。

保存与运输

• 保存建议：未启封的试剂盒请避光保存于2-8℃，保质期6个月。启用后，请参照说明书妥善保存各组分。
• 运输条件：全程蓝冰（冰袋）运输，确保试剂活性。

订购信息

重要注意事项

• 请勿将不同批次的试剂或已过有效期的试剂混合使用。
• 使用前，所有试剂应在室温平衡约30分钟。
• 实验过程中请使用一次性耗材，以避免交叉污染。
• 为确保结果准确性，建议对样品和标准品进行复孔（双孔或三孔）检测。
• 充分的混匀（推荐使用低速振荡器或定期手动摇匀）对实验结果的均一性至关重要。

警告： 本产品仅限于科学研究用途，不适用于人类或临床诊断。使用前请确保符合所有相关的法律法规和许可要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

The KTE4024-2 EliKine™ Mouse Inducible Nitric Oxide Synthase (iNOS) ELISA Kit is a high-quality detection tool developed based on the well-established double-antibody sandwich principle. It is specifically designed for the accurate quantification of iNOS protein concentrations in samples such as mouse serum, plasma, tissue homogenates, and cell culture supernatants. As a core product within the Cell Analysis product portfolio, this kit strictly adheres to ELISA application standards, providing reliable data support for scientific research in fields such as inflammation, immune response, and cardiovascular diseases.

Core Features & Advantages

• High Sensitivity & Broad Linear Range: The minimum detectable concentration is as low as 8 pg/mL, with a detection range spanning 40 pg/mL - 3200 pg/mL, ensuring accurate quantitative results across a wide concentration interval.
• Excellent Specificity: Rigorously validated for high specificity towards the target protein iNOS, with no significant cross-reactivity observed with analogues, effectively minimizing background interference and ensuring reliable experimental data.
• Convenient One-Step Operation: The optimized experimental procedure (one-step method) simplifies operational steps, enhancing experimental efficiency and reducing potential errors associated with multi-step processes.
• Ready-to-Use Complete Components: The kit provides all necessary components, including pre-coated microplate, standard, detection antibody, enzyme conjugate, chromogenic substrate, and wash buffer, ready for immediate use.

Technical Parameters

Kit Components (48T/96T)

• Mouse iNOS Pre-coated Microplate
• Mouse iNOS Standard (6,400 pg/mL)
• Standard Diluent
• Mouse iNOS Detection Antibody (Biotinylated)
• Streptavidin-HRP
• HRP Chromogenic Substrate Solution A & B (TMB)
• Stop Solution
• Wash Buffer (Concentrated)
• Plate Sealer

(Note: Specific component quantities may vary depending on the kit size; please refer to the actual kit manual received.)

Background Information

Inducible Nitric Oxide Synthase (iNOS, encoded by the NOS2 gene) is a key enzyme that catalyzes the production of nitric oxide (NO) from L-arginine. It is primarily induced by inflammatory factors in immune cells such as macrophages, playing a crucial role in host defense, inflammatory responses, and cellular signal transduction. Accurate detection of iNOS expression levels is essential for in-depth research into related pathophysiological processes.

Storage & Shipping

• Storage Recommendation: Store the unopened kit protected from light at 2-8°C; the shelf life is 6 months. After opening, store individual components properly according to the manual.
• Shipping Conditions: Shipped with blue ice (ice packs) to ensure reagent activity.

Ordering Information

Important Notes

• Do not mix reagents from different batches or use expired reagents.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable consumables during the experiment to avoid cross-contamination.
• To ensure result accuracy, it is recommended to perform duplicate or triplicate measurements for both samples and standards.
• Thorough mixing (using a low-speed shaker or periodic manual shaking is recommended) is crucial for the uniformity of experimental results.

Warning: This product is intended for research use only, not for human or clinical diagnostic use. Please ensure compliance with all relevant laws, regulations, and licensing requirements before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.