MMP-9 ELISA KIT 总基质金属蛋白酶9ELISA

产品概述

英文名称：Mouse Total MMP-9 ELISA Kit
中文名称：MMP-9 ELISA KIT 总基质金属蛋白酶9 ELISA 试剂盒（小鼠）
货号：KTE3033-2
检测类型：ELISA（双抗体夹心法）
反应物种：小鼠

关键特性 & 优势

• 高灵敏度：检测下限 0.16 ng/mL，覆盖 0.16–10 ng/mL 动态范围。
• 特异性强：采用预包被高亲和力抗体，未检测到显著交叉反应或背景干扰。
• 多样样本适用：细胞培养上清、血清、血浆均可直接检测。
• 成熟的 EliKine™ 平台：兼容常规酶标仪，读取 450 nm（参考 570–630 nm）波长。
• 方便的试剂配置：所有关键试剂均已预稀释或标记，减少实验准备时间。

适用范围与检测原理

基质金属蛋白酶 9（MMP‑9）是参与细胞外基质重塑、血管生成、炎症及肿瘤转移的重要酶。该套件通过双抗体夹心酶联免疫吸附（ELISA）技术定量小鼠全长 MMP‑9（total MMP‑9），包括活性酶形态和前体形态。

检测流程概述：

1. 标准品、待测样本和生物素化检测抗体加入预包被抗体的酶标板孔中，孵育形成抗原—捕获抗体—检测抗体三元复合物。
2. 洗涤除去未结合组分。
3. 加入链霉亲和素‑HRP（Streptavidin‑HRP），与生物素标记的检测抗体结合。
4. 洗涤后加入 TMB 底物，显色反应与样本中 MMP‑9 浓度成正比。
5. 加入终止液，使用酶标仪在 450 nm（参考 570–630 nm）读取吸光度，绘制标准曲线并计算样本浓度。

试剂盒组成

组件	数量/浓度
Mouse MMP-9 Microplate	预包被 96 孔酶标板（48 T 或 96 T）
Mouse MMP-9 Standard (lyophilized)	5 × 标准品（0.16–10 ng/mL）
Standard Diluent	1 × 稀释缓冲液
Assay Buffer (10×)	稀释至工作浓度
Mouse MMP-9 Detect Antibody (100×)	生物素化检测抗体
Streptavidin‑HRP (100×)	链霉亲和素‑HRP
HRP Substrate（TMB）	显色底物
Stop Solution	终止液
Wash Buffer (20×)	清洗缓冲液
Plate Covers	防尘盖

操作要点

• 所有试剂在使用前需在室温（≈25 °C）平衡 30 分钟。
• 实验期间推荐使用一次性吸头，防止交叉污染。
• 洗涤步骤务必充分，可使用低速振荡器或每 10 分钟手动轻摇一次。
• 标准品与样本建议设置双孔或三孔复孔，以提高数据可靠性。
• 未使用的酶标板应立即密封并在 4 °C 保存，以防止活性下降。

保存与运输

• 未开封试剂盒：避光保存于 2–8 °C，保质期 24 个月。
• 开启后请参考说明书中各组分的具体保存条件。
• 运输方式：冷链（蓝冰包装），确保全程维持低温。

规格 & 价格

规格	盒数	单价（元）	交货期
48 T	1 盒	1598.00	请咨询
96 T	1 盒	2558.00	请咨询

注意事项 & 警示

• 同批号试剂方可混用，避免使用过期或不同批号的试剂混合。
• 本产品仅限科研用途，不适用于人类或临床诊断。
• 使用本套件时，请遵守相关专利和许可证规定，因侵权或违规行为导致的后果，本公司概不负责。

背景信息（供参考）

基质金属蛋白酶 9（MMP‑9），亦称 92 kDa IV 型胶原酶或明胶酶 B，属于锌金属蛋白酶家族。它在胚胎发育、血管生成、伤口愈合以及细胞迁移等生理过程中发挥关键作用；在关节炎、脑出血、肿瘤转移、心血管疾病等病理状态中常呈升高趋势。通过本套件定量小鼠全长 MMP‑9，可为疾病模型、药物干预及机制研究提供可靠的蛋白水平信息。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Mouse Total MMP-9 ELISA Kit
Chinese Name: MMP-9 ELISA KIT Total Matrix Metalloproteinase‑9 ELISA Reagent Kit (Mouse)
Catalog No.: KTE3033-2
Assay Type: ELISA (sandwich)
Species: Mouse

Key Features & Advantages

• High Sensitivity: Lower limit of detection 0.16 ng/mL, covering a dynamic range of 0.16–10 ng/mL.
• Strong Specificity: Utilizes pre‑coated high‑affinity antibodies with no significant cross‑reactivity or background interference detected.
• Versatile Sample Compatibility: Cell culture supernatants, serum, and plasma can be analyzed directly.
• Mature EliKine™ Platform: Compatible with standard microplate readers, reading at 450 nm (reference 570–630 nm).
• Convenient Reagent Configuration: All critical reagents are pre‑diluted or pre‑labeled, reducing assay setup time.

Application Scope & Assay Principle

Matrix metalloproteinase‑9 (MMP‑9) is a key enzyme involved in extracellular matrix remodeling, angiogenesis, inflammation, and tumor metastasis. This kit quantifies total mouse MMP‑9 (including both active and pro‑enzyme forms) using a sandwich enzyme‑linked immunosorbent assay (ELISA) format.

Assay workflow overview:

1. Standards, test samples, and biotinylated detection antibody are added to wells pre‑coated with capture antibody and incubated to form the antigen–capture antibody–detection antibody ternary complex.
2. Wash to remove unbound components.
3. Add Streptavidin‑HRP, which binds to the biotin‑labeled detection antibody.
4. After washing, add TMB substrate; color development is proportional to the MMP‑9 concentration in the sample.
5. Add stop solution and read absorbance on a microplate reader at 450 nm (reference 570–630 nm); generate a standard curve and calculate sample concentrations.

Kit Components

Component	Quantity / Concentration
Mouse MMP-9 Microplate	Pre‑coated 96‑well microplate (48 T or 96 T)
Mouse MMP-9 Standard (lyophilized)	5 × standards (0.16–10 ng/mL)
Standard Diluent	1× dilution buffer
Assay Buffer (10×)	Dilute to working concentration
Mouse MMP-9 Detect Antibody (100×)	Biotinylated detection antibody
Streptavidin‑HRP (100×)	Streptavidin‑HRP
HRP Substrate（TMB）	Chromogenic substrate
Stop Solution	Stop solution
Wash Buffer (20×)	Wash buffer (20×)
Plate Covers	Plate seals

Operational Tips

• All reagents should equilibrate at room temperature (≈25 °C) for 30 minutes before use.
• Use disposable pipette tips throughout the assay to prevent cross‑contamination.
• Ensure thorough washing; a low‑speed shaker may be used, or gently tap the plate manually every 10 minutes.
• Run standards and samples in duplicate or triplicate to enhance data reliability.
• Seal any unused microplates promptly and store at 4 °C to preserve activity.

Storage & Shipping

• Unopened kits: store protected from light at 2–8 °C; shelf life 24 months.
• After opening, refer to the user manual for specific storage conditions of each component.
• Shipping: Cold chain (blue ice) to maintain low temperature throughout transit.

Specifications & Price

Specification	Units per Box	Unit Price (CNY)	Lead Time
48 T	1 box	1598.00	Contact for quote
96 T	1 box	2558.00	Contact for quote

Precautions & Warnings

• Only reagents from the same lot may be mixed; avoid using expired or different‑lot reagents together.
• This product is for research use only and is not intended for human or clinical diagnostic applications.
• When using this kit, comply with all applicable patents and licensing terms; the company assumes no responsibility for any infringement or violations.

Background Information (For Reference)

Matrix metalloproteinase‑9 (MMP‑9), also known as 92 kDa type IV collagenase or gelatinase B, belongs to the zinc‑dependent metalloproteinase family. It plays critical roles in embryonic development, angiogenesis, wound healing, and cell migration; its levels are often elevated in pathological conditions such as arthritis, intracerebral hemorrhage, tumor metastasis, and cardiovascular diseases. Quantification of total mouse MMP‑9 with this kit provides reliable protein level data for disease models, therapeutic interventions, and mechanistic studies.

Note: The above information is subject to change due to product optimizations or upgrades; please contact Yakeyin Co. for the most current details.