小鼠粒细胞-巨噬细胞集落刺激因子 ELISA定量试剂盒(GM

小鼠粒细胞-巨噬细胞集落刺激因子 (GM‑CSF) ELISA 定量试剂盒

英文名称：Mouse GM‑CSF ELISA Kit | 货号：KTE7002‑1

产品定位

• 应用领域：蛋白类检测，专用于 ELISA 夹心法
• 一级应用：ELISA（酶联免疫吸附测定）
• 产品分类：核心产品 – 细胞分析
• 适用物种：小鼠（Mouse）

检测概述

本套件采用高灵敏度夹心 ELISA 体系，实现对小鼠 GM‑CSF 的定量检测。适用于细胞培养上清、血清、血浆及其它生物液体样本，检测范围 7.8 pg/mL – 500 pg/mL，检出限 4 pg/mL。

主要成分

• 小鼠源 GM‑CSF 抗体预包被微孔板
• 小鼠源 GM‑CSF 蛋白标准品
• 小鼠源 GM‑CSF 检测抗体
• Streptavidin‑HRP 复合物
• 标准品稀释液
• 实验缓冲液
• HRP 底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

特点 & 优势

• 极高的灵敏度和特异性，检出限达 4 pg/mL
• 对同源蛋白（如人 GM‑CSF）无显著交叉反应，背景干扰低
• 预包被微孔板省去抗体包被步骤，提高实验一致性
• 配套完整，所有关键缓冲液均已预配好，适合高通量实验

实验注意事项

• 不同批号或已过期的试剂不得混用。
• 使用前请在室温（≈25 ℃）平衡 30 分钟。
• 全程使用一次性吸头，防止交叉污染。
• 未用完的板条请立即回装、密封，置于 4 ℃保存。
• 混匀是获取可靠结果的关键，推荐使用低速振荡器或每 10 分钟手动轻摇。
• 样品与标准品建议设定双孔或三孔（复孔）以提升数据可靠性。

储存与运输

• 保存建议：未开封试剂盒请存于 2‑8 ℃；开封后请参照说明书分别保存各组分。
• 运输条件：冰袋（蓝冰）冷链运输，确保活性维持。

警告声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本套件进行任何专利或许可证受限的操作所导致的侵权或违规行为，供应商不承担责任。

技术背景

粒‑巨细胞集落刺激因子（GM‑CSF）是一种多效性细胞因子，能够刺激造血干细胞的增殖、成熟与分化。小鼠 GM‑CSF（141 aa）与人 GM‑CSF（144 aa）在氨基酸水平上同源率约 54%，但生物学活性表现出种属特异性。GM‑CSF 与前列腺癌、甲状腺癌、真菌感染、艾滋病、骨髓移植、肾细胞癌、肺部炎症及血液系统恶性肿瘤等疾病密切相关，是免疫调节及疾病模型研究的重要指标。

关键检索信息

• 基因 ID：12981
• 别名：GM‑CSF, CSF2, Colony stimulating factor 等
• 蛋白质 ID：P01587 (UniProt)

规格与报价

规格	单价（人民币）	货期
48 T	1,598.00	现货（次日发货）
96 T	2,558.00	现货（次日发货）

检验指标

检测分子：GM‑CSF（小鼠）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Granulocyte-Macrophage Colony-Stimulating Factor (GM‑CSF) ELISA Quantification Kit

English Name: Mouse GM‑CSF ELISA Kit | Catalog No.: KTE7002‑1

Product Positioning

• Application Area: Protein detection, specifically for sandwich ELISA
• Primary Application: ELISA (Enzyme-Linked Immunosorbent Assay)
• Product Category: Core Product – Cell Analysis
• Applicable Species: Mouse

Assay Overview

This kit utilizes a high‑sensitivity sandwich ELISA format to quantitatively measure mouse GM‑CSF. It is suitable for cell culture supernatants, serum, plasma, and other biological fluid samples, with a detection range of 7.8 pg/mL – 500 pg/mL and a limit of detection of 4 pg/mL.

Key Components

• Pre‑coated microplate with mouse GM‑CSF capture antibody
• Mouse GM‑CSF recombinant protein standard
• Mouse GM‑CSF detection antibody
• Streptavidin‑HRP conjugate
• Standard dilution buffer
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate sealing film

Features & Advantages

• Ultra‑high sensitivity and specificity, LOD down to 4 pg/mL
• No significant cross‑reactivity with homologous proteins (e.g., human GM‑CSF); low background interference
• Pre‑coated plates eliminate the coating step, improving assay consistency
• Complete reagent set with all critical buffers pre‑prepared, suitable for high‑throughput workflows

Experimental Considerations

• Do not mix reagents from different lot numbers or expired kits.
• Allow all components to equilibrate at room temperature (≈25 °C) for 30 minutes before use.
• Use disposable tips throughout to prevent cross‑contamination.
• Seal and store unused plate strips immediately at 4 °C.
• Mixing is critical for reliable results; recommend gentle vortexing or manual inversion every 10 minutes.
• Run samples and standards in duplicate or triplicate wells to enhance data reliability.

Storage & Shipping

• Storage Recommendation: Keep unopened kits at 2‑8 °C; after opening, store individual components as directed in the manual.
• Shipping Conditions: Cold‑chain transport with ice packs (blue ice) to preserve activity.

Warning Statement

This product is for research use only and is not intended for human or clinical diagnostic applications. The supplier assumes no liability for any infringement or regulatory violations arising from the use of this kit in patented or license‑restricted procedures.

Technical Background

Granulocyte‑macrophage colony‑stimulating factor (GM‑CSF) is a pleiotropic cytokine that drives proliferation, maturation, and differentiation of hematopoietic stem cells. Mouse GM‑CSF (141 aa) shares ~54 % amino‑acid identity with human GM‑CSF (144 aa), yet its biological activity is species‑specific. GM‑CSF is closely linked to prostate cancer, thyroid cancer, fungal infections, AIDS, bone‑marrow transplantation, renal cell carcinoma, pulmonary inflammation, and hematologic malignancies, making it a key biomarker for immune modulation and disease‑model research.

Key Retrieval Information

• Gene ID: 12981
• Synonyms: GM‑CSF, CSF2, colony stimulating factor, etc.
• Protein ID: P01587 (UniProt)

Specifications & Pricing

Specification	Unit Price (CNY)	Lead Time
48 T	1,598.00	In stock (next‑day shipment)
96 T	2,558.00	In stock (next‑day shipment)

Assay Parameters

Analyte: GM‑CSF (Mouse)

Note: Information is subject to change due to product upgrades or other factors; please contact Yakeyin Co. for the latest details.