小鼠酸性成纤维细胞生长因子 ELISA定量试剂盒(AFGF/

产品概述

EliKine™ 小鼠酸性成纤维细胞生长因子（FGF1）ELISA定量试剂盒是一款专为科研领域设计的高性能检测工具，采用先进的双抗体夹心一步法原理，精准定量小鼠血清、血浆、组织匀浆及细胞上清等样本中的FGF1蛋白含量。该试剂盒隶属于细胞分析产品中心的核心产品线，为ELISA应用提供了可靠的技术支持。

核心特性

• 高灵敏度：最低检测浓度可达2.5 pg/mL，满足低丰度样本的检测需求。
• 宽线性范围：检测范围为12.5 pg/mL至1,000 pg/mL，适用于不同浓度梯度的样本分析。
• 优异特异性：经严格验证，与目标蛋白类似物无显著交叉反应，背景干扰低。
• 操作便捷：优化的一步法流程缩短了实验时间，提升了工作效率。

检测原理

本试剂盒采用双抗体夹心ELISA技术。预先包被于微孔板中的特异性抗体捕获样本中的FGF1，随后依次加入生物素化检测抗体与链霉亲和素-HRP复合物，形成稳定的免疫复合物。经TMB显色后，在450 nm波长下测定吸光度值，FGF1浓度与OD值呈正相关，实现精准定量。

试剂盒组分

技术参数

• 产品货号：KTE4001-1
• 反应种属：小鼠
• 样本类型：血清、血浆、组织匀浆、细胞培养上清及相关液体样本
• 检测方法：双抗体夹心一步法ELISA
• 灵敏度：2.5 pg/mL
• 检测范围：12.5-1,000 pg/mL

规格与价格

保存与运输

未开封试剂盒避光保存于2-8℃，有效期为6个月。开封后请参照说明书保存各组分。本品采用蓝冰冰袋运输，确保试剂活性。

注意事项

• 请勿混用不同批号或过期试剂。
• 使用前需室温平衡30分钟，避免交叉污染。
• 建议样本与标准品做复孔检测以提高准确性。
• 实验过程中需充分混匀反应体系。

应用背景

酸性成纤维细胞生长因子（FGF1）在细胞增殖、分化及组织修复中发挥关键作用。本试剂盒适用于心血管疾病、肿瘤学、发育生物学等领域的机制研究，为探索FGF1功能提供精准的定量工具。

警告：本品仅供科研使用，不适用于临床诊断。使用者应确保应用符合相关法规及知识产权要求。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

The EliKine™ Mouse Acidic Fibroblast Growth Factor (FGF1) ELISA Quantitation Kit is a high-performance detection tool specifically designed for scientific research. Utilizing the advanced one-step sandwich ELISA principle, it enables accurate quantitation of FGF1 protein levels in samples such as mouse serum, plasma, tissue homogenates, and cell culture supernatants. This kit is a core product within the Cell Analysis Product Center, providing reliable technical support for ELISA applications.

Key Features

• High Sensitivity: The minimum detectable concentration can reach 2.5 pg/mL, meeting the detection needs for low-abundance samples.
• Wide Linear Range: The detection range is 12.5 pg/mL to 1,000 pg/mL, suitable for analyzing samples with varying concentration gradients.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity with analogs of the target protein, resulting in low background interference.
• User-Friendly Operation: The optimized one-step procedure shortens experimental time and improves work efficiency.

Assay Principle

This kit employs a sandwich ELISA technique. A specific antibody pre-coated onto the microplate captures FGF1 from the sample. Subsequently, a biotinylated detection antibody and a Streptavidin-HRP conjugate are added sequentially to form a stable immune complex. After TMB substrate development, the absorbance is measured at 450 nm. The FGF1 concentration is directly proportional to the OD value, enabling precise quantitation.

Kit Components

Technical Parameters

• Catalog Number: KTE4001-1
• Reactivity: Mouse
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants, and related liquid samples
• Assay Method: One-step Sandwich ELISA
• Sensitivity: 2.5 pg/mL
• Detection Range: 12.5-1,000 pg/mL

Specifications & Pricing

Storage & Shipping

The unopened kit should be stored protected from light at 2-8°C and is stable for 6 months. After opening, please store individual components according to the manual. The product is shipped with blue ice packs to ensure reagent activity.

Precautions

• Do not mix reagents from different lot numbers or use expired reagents.
• Allow all components to equilibrate to room temperature for 30 minutes before use. Avoid cross-contamination.
• It is recommended to perform duplicate assays for both samples and standards to improve accuracy.
• Ensure thorough mixing of the reaction system during the assay procedure.

Background

Acidic Fibroblast Growth Factor (FGF1) plays a key role in cell proliferation, differentiation, and tissue repair. This kit is suitable for mechanism studies in fields such as cardiovascular disease, oncology, and developmental biology, providing a precise quantitation tool for exploring FGF1 function.

Warning: This product is for research use only. Not intended for clinical diagnosis. Users should ensure their application complies with relevant regulations and intellectual property requirements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.