小鼠白介素17A ELISA定量试剂盒(IL-17A)

小鼠白介素17A ELISA定量试剂盒 (IL-17A) - KTE3027-1

产品概述

本试剂盒为EliKine™系列核心产品，专为精准定量小鼠样本（包括细胞培养上清、血清及血浆）中的白介素17A (IL-17A) 蛋白而设计。采用成熟的夹心法ELISA原理，确保了检测的高特异性和可靠性，适用于免疫学、炎症机制、药物筛选等领域的深入研究。

核心特性与优势

• 高灵敏度：检测下限低至31.25 pg/mL，能够精准检测低丰度表达样本。
• 优异特异性：经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰。
• 宽线性范围：检测范围为31.25 pg/mL - 2,000 pg/mL，满足多样本浓度需求。
• 操作便捷：试剂盒提供预包被板条及即用型组分，优化实验流程，节省准备时间。
• 批次稳定性：严格质量控制，确保不同批次间结果的一致性。

产品规格与订购信息

规格	货号	价格 (元)	货期
48 T	KTE3027-1	1,598.0	期货（5天内发货）
96 T	KTE3027-1	2,558.0	期货（5天内发货）

试剂盒组分

• Mouse IL-17A Microplate (预包被微孔板)
• Mouse IL-17A Standard (冻干标准品)
• Standard Diluent (标准品稀释液)
• Assay Buffer (10×) (检测缓冲液)
• Mouse IL-17A Detect Antibody (100×) (检测抗体)
• Streptavidin-HRP (100×) (链霉亲和素-HRP)
• HRP Substrate (TMB) (HRP底物TMB)
• Stop Solution (终止液)
• Wash Buffer (20×) (洗涤缓冲液)
• Plate Covers (板盖)

注：详细使用比例与配制方法请参阅产品说明书。

检测原理简介

本试剂盒基于双抗体夹心ELISA法。包被于板上的捕获抗体特异结合样本中的小鼠IL-17A，随后依次加入生物素化检测抗体、链霉亲和素-HRP偶联物及TMB底物进行显色。显色强度与样本中IL-17A浓度成正比，通过标准曲线实现精准定量。

样本处理与保存

适用于细胞培养上清、血清及血浆样本。建议样本采集后尽快分离，避免反复冻融。若需长期保存，请于-20℃或更低温度下冻存，并避免使用溶血或高脂血样本。

保存与运输

• 保存建议：未开封试剂盒请避光保存于2-8℃，保质期24个月。启用后，各组分请按说明书建议条件保存。
• 运输条件：全程蓝冰（冰袋）运输，确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或过期试剂。
• 使用前所有试剂需室温平衡30分钟。
• 实验过程中请使用一次性耗材，严防交叉污染。
• 未使用的板条须立即密封，4℃保存。
• 推荐使用振荡器混匀或定期手动摇匀，确保反应充分。
• 为保障结果准确性，标准品与样品建议进行复孔检测。

背景知识

白细胞介素17A (IL-17A) 是IL-17细胞因子家族的关键成员，主要由Th17细胞产生，在宿主防御、炎症反应及自身免疫性疾病（如类风湿关节炎、牛皮癣、多发性硬化症）中发挥核心作用。其通过诱导趋化因子产生，招募中性粒细胞等免疫细胞至炎症部位，放大炎症信号。

使用声明

本产品仅限于科研用途，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或许可规定，厂家不对因使用本产品可能导致的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse Interleukin‑17A ELISA Quantitation Kit (IL‑17A) – KTE3027‑1

Product Overview

This kit is a core product of the EliKine™ series, specifically designed for the precise quantification of mouse interleukin‑17A (IL‑17A) protein in various mouse matrices (including cell‑culture supernatants, serum, and plasma). It employs a mature sandwich‑ELISA format, delivering high specificity and reliability, and is suitable for in‑depth research in immunology, inflammation mechanisms, drug screening, and related fields.

Key Features and Benefits

• High Sensitivity: Lower limit of detection as low as 31.25 pg/mL, enabling accurate measurement of low‑abundance samples.
• Excellent Specificity: Rigorously validated with negligible cross‑reactivity or background interference from homologous proteins.
• Broad Linear Range: Quantifiable range of 31.25 pg/mL – 2,000 pg/mL to accommodate diverse sample concentrations.
• User‑Friendly Workflow: Kit includes pre‑coated plates and ready‑to‑use reagents, streamlining the assay and reducing preparation time.
• Batch‑to‑Batch Consistency: Strict quality control ensures reproducibility across different production lots.

Product Specifications and Ordering Information

Specification	Catalog No.	Price (CNY)	Lead Time
48 T	KTE3027‑1	1,598.0	In‑stock (ship within 5 days)
96 T	KTE3027‑1	2,558.0	In‑stock (ship within 5 days)

Kit Components

• Mouse IL‑17A Microplate (pre‑coated)
• Mouse IL‑17A Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Mouse IL‑17A Detection Antibody (100×)
• Streptavidin‑HRP Conjugate (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Note: Detailed reagent ratios and preparation procedures are provided in the product manual.

Assay Principle Overview

The kit utilizes a dual‑antibody sandwich ELISA format. The capture antibody coated on the plate specifically binds mouse IL‑17A present in the sample. This is followed by the sequential addition of a biotinylated detection antibody, streptavidin‑HRP conjugate, and TMB substrate for color development. The intensity of the colorimetric signal is directly proportional to the IL‑17A concentration in the sample and is quantified using a standard curve.

Sample Handling and Storage

Applicable to cell‑culture supernatants, serum, and plasma. It is recommended to process samples promptly after collection and to avoid repeated freeze‑thaw cycles. For long‑term storage, aliquot and freeze at –20 °C or lower, and avoid hemolyzed or highly lipemic specimens.

Storage and Shipping

• Storage Recommendation: Unopened kits should be kept protected from light at 2 – 8 °C, with a shelf life of 24 months. Once opened, each component should be stored according to the conditions specified in the manual.
• Shipping Conditions: Transported on dry ice or with sufficient ice packs to maintain reagent activity.

Critical Precautions

• Do not mix reagents from different lot numbers or use expired components.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable consumables throughout the assay to prevent cross‑contamination.
• Seal unused plates immediately and store at 4 °C.
• Mix reagents by gentle vortexing or periodic manual agitation to ensure homogeneity.
• Run standards and samples in duplicate to ensure data reliability.

Background Information

Interleukin‑17A (IL‑17A) is a pivotal member of the IL‑17 cytokine family, predominantly produced by Th17 cells. It plays a central role in host defense, inflammatory responses, and autoimmune diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis. IL‑17A exerts its effects by inducing chemokine production, thereby recruiting neutrophils and other immune cells to sites of inflammation and amplifying the inflammatory cascade.

Usage Statement

This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licenses; the manufacturer assumes no liability for any patent infringement or other regulatory violations arising from product usage.

Note: The information above is subject to change due to product optimization or upgrades. For the most current details, please contact EliKine.