白介素10酶联免疫试剂盒(大鼠) IL-10 ELISA K

Rat IL-10 ELISA Kit (KTE3039-3)

中文名称：白介素10酶联免疫试剂盒（大鼠）
英文名称：Rat IL-10 ELISA Kit
产品分类：核心产品 | 细胞分析 | ELISA
适用技术：ELISA（夹心法）
目标分子：IL-10（大鼠）
检测范围：62.5 pg/mL – 4,000 pg/mL
灵敏度：62.5 pg/mL

一、产品概述

EliKine™ Rat IL‑10 ELISA Kit 采用双抗体夹心技术，专为大鼠细胞培养上清、血清及血浆中的 IL‑10 定量设计。高亲和力的预包被抗体以及生物素化检测试剂实现了很好的灵敏度与特异性，适用于基础免疫学、炎症机制探索及药物筛选等研究领域。

二、关键优势

• 广泛的线性检测范围（62.5 pg/mL – 4,000 pg/mL），覆盖低至亚纳克浓度的表达水平。
• 采用高亲和力预包被微孔板，显著降低背景信号。
• 对 IL‑10 同源蛋白及其他细胞因子未观察到明显交叉反应。
• 试剂稳定性佳，推荐在 2‑8 ℃、避光保存。
• 套装内含全套实验耗材，避免额外采购。

三、试剂盒组成

• Rat IL-10 Microplate（预包被）
• Rat IL-10 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat IL-10 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

四、技术参数与规格

项目	规格	单价（人民币）	交货时间
套装容量	48 T	1,598.00	期货（5 天内发货）
套装容量	96 T	2,558.00	期货（5 天内发货）

五、实验流程要点（推荐）

1. 按说明书将所有试剂恢复至室温（30 分钟）。
2. 使用一次性吸头进行加样，建议每个样本设双孔或三孔复孔。
3. 在每一步孵育后使用 20× Wash Buffer 彻底洗涤，确保去除非特异性结合。
4. 显色后添加 Stop Solution，使用 450 nm（参考 570‑630 nm）读取吸光度。
5. 使用标准曲线进行样本浓度计算。

六、注意事项

• 不同批号或过期的试剂请勿混用。
• 操作中请使用低速振荡或每10 分钟手动摇匀，确保反应均一。
• 未使用的板凳请密封保存于4 ℃，避免光照。
• 实验结束后请及时清理，防止交叉污染。

七、存储与运输

未开封试剂盒：避光保存于 2‑8 ℃，有效期 24 个月。

已开封组分：请参照说明书中各组分的具体保存要求。

运输方式：采用蓝冰（冰袋）冷链运输，确保全程温度控制。

八、合规声明

本产品仅供科研使用，不适用于人类或临床诊断。使用本套件进行的任何专利或许可相关操作均需自行确认合法性，生产厂家不承担因使用本产品导致的专利侵权或其他法律责任。

九、产品背景简述

白介素10（IL‑10）是由两条 178 氨基酸链形成的同源二聚体，主要由单核细胞、调节性 T 细胞等产生，发挥免疫抑制和抗炎作用。IL‑10 能下调巨噬细胞的 Th1 细胞因子、抑制 NF‑κB 活性，并通过 JAK‑STAT 通路调控细胞功能。因其免疫调节特性，IL‑10 在移植排斥、炎症模型以及免疫治疗研究中具有重要价值。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat IL-10 ELISA Kit (KTE3039-3)

Chinese Name: Interleukin 10 Enzyme-Linked Immunosorbent Assay Kit (Rat)
English Name: Rat IL-10 ELISA Kit
Product Category: Core Products | Cell Analysis | ELISA
Suitable Technology: ELISA (Sandwich Method)
Target Molecule: IL-10 (Rat)
Detection Range: 62.5 pg/mL – 4,000 pg/mL
Sensitivity: 62.5 pg/mL

1. Product Overview

The EliKine™ Rat IL‑10 ELISA Kit employs a double-antibody sandwich technology, specifically designed for the quantitative detection of IL‑10 in rat cell culture supernatants, serum, and plasma. High-affinity pre-coated antibodies and biotinylated detection reagents ensure excellent sensitivity and specificity, making it suitable for research areas such as basic immunology, inflammation mechanism exploration, and drug screening.

2. Key Advantages

• Wide linear detection range (62.5 pg/mL – 4,000 pg/mL), covering expression levels down to sub-nanogram concentrations.
• Utilizes high-affinity pre-coated microplates, significantly reducing background signal.
• No significant cross-reactivity observed with IL‑10 homologous proteins or other cytokines.
• Excellent reagent stability, recommended for storage at 2‑8 ℃, protected from light.
• The kit includes a complete set of experimental consumables, eliminating the need for additional purchases.

3. Kit Components

• Rat IL-10 Microplate (Pre-coated)
• Rat IL-10 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Rat IL-10 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

4. Technical Parameters and Specifications

Item	Specification	Unit Price (RMB)	Delivery Time
Kit Size	48 T	1,598.00	Future Goods (Ships within 5 days)
Kit Size	96 T	2,558.00	Future Goods (Ships within 5 days)

5. Key Experimental Procedure (Recommended)

1. Allow all reagents to equilibrate to room temperature (30 minutes) as per the instructions.
2. Use disposable pipette tips for sample addition; it is recommended to set up duplicate or triplicate wells for each sample.
3. Thoroughly wash after each incubation step using the 20× Wash Buffer to remove non-specific binding.
4. After color development, add the Stop Solution and read the absorbance at 450 nm (reference wavelength 570‑630 nm).
5. Calculate sample concentrations using the standard curve.

6. Precautions

• Do not mix reagents from different lot numbers or expired reagents.
• Use low-speed shaking or manually shake every 10 minutes during the procedure to ensure uniform reaction.
• Seal and store unused wells at 4 ℃, protected from light.
• Clean up promptly after the experiment to prevent cross-contamination.

7. Storage and Shipping

Unopened Kit: Store protected from light at 2‑8 ℃; shelf life is 24 months.

Opened Components: Please refer to the specific storage requirements for each component in the instructions.

Shipping Method: Shipped via cold chain using blue ice (ice packs) to ensure temperature control throughout the process.

8. Compliance Statement

This product is for research use only and is not intended for human or clinical diagnostic use. Any operations related to patents or licenses conducted using this kit require self-verification of legality; the manufacturer is not liable for patent infringement or other legal responsibilities arising from the use of this product.

9. Product Background Summary

Interleukin-10 (IL‑10) is a homodimer formed by two 178-amino acid chains, primarily produced by monocytes, regulatory T cells, etc., exerting immunosuppressive and anti-inflammatory effects. IL‑10 can downregulate Th1 cytokines in macrophages, inhibit NF‑κB activity, and regulate cell function through the JAK-STAT pathway. Due to its immunomodulatory properties, IL‑10 holds significant value in research on transplant rejection, inflammation models, and immunotherapy.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.