IL-4 ELISA KIT 白介素-4ELISA试剂盒(大

产品概览

英文名称：Rat IL-4 ELISA Kit
中文名称：IL-4 ELISA KIT 白介素-4 ELISA试剂盒（大鼠）
货号：KTE9003-2
检测类型：ELISA（夹心法）
适用样本：细胞培养上清、血清、血浆及其他生物液体

核心应用领域

本试剂盒专为大鼠 IL‑4 蛋白的定量检测设计，广泛用于免疫学、细胞因子研究以及肿瘤免疫调节机制的探索。

一级应用

• ELISA 应用
• 细胞分析中心核心产品

试剂盒组成

• 大鼠源 IL‑4 抗体预包被微孔板
• 大鼠源 IL‑4 蛋白标准品
• 大鼠源 IL‑4 检测抗体
• EliKine™ Streptavidin‑HRP
• 标准品稀释液
• 实验缓冲液
• HRP 底物
• 反应终止液
• 洗涤缓冲液
• 封板膜

性能指标

检测范围	7.5 pg/mL – 100 pg/mL
灵敏度（检测下限）	5 pg/mL
特异性	无显著交叉反应或背景干扰（已在大鼠样本中验证）

使用说明要点

• 同批次试剂方可混用，避免跨批号或过期试剂混合。
• 使用前请于室温平衡 30 分钟。
• 实验操作建议使用一次性吸头，防止交叉污染。
• 未使用的板条请立即放回原包装，密封后 4 ℃保存。
• 充分混匀对结果尤为关键，推荐低速振荡或每 10 分钟手动轻摇。
• 样品和标准品建议设双孔或三孔复孔，以提高数据可靠性。

存储与运输

• 未开封试剂盒请存放于 2–8 ℃（冰箱），避免冻融循环。
• 启用后请参照说明书对各组分进行分类保存。
• 运输采用蓝色冰袋（冷链）方式，确保全程低温。

规格与价格

规格	单价 (CNY)	货期
48 T（48 套）	1,598.0	现货（次日发货）
96 T（96 套）	2,558.0	现货（次日发货）

法规声明

警告：本产品仅用于科研目的，不适用于人类临床诊断。使用本产品进行任何专利或许可证的侵权行为，风险由使用方自行承担。

产品背景

白细胞介素‑4（IL‑4）是由活化的 T 淋巴细胞、肥大细胞和嗜碱性粒细胞分泌的多功能细胞因子。它在调节免疫响应、促进 B 细胞增殖以及介导抗肿瘤活性方面发挥重要作用。针对大鼠模型的 IL‑4 定量检测，可帮助研究者解析免疫调节网络、评估药物干预效果以及开展新型免疫治疗的前期探索。

基因与蛋白信息

• 基因 ID：287287
• 蛋白质 ID：P20096
• 别名：IL4, BCGF‑1, BCGF1, BSF1, MGC79402, B cell growth factor 1, B_cell stimulatory factor 1, lymphocyte stimulatory factor 1
• 蛋白序列链接：UniProt - P20096

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Rat IL-4 ELISA Kit
Chinese Name: IL-4 ELISA KIT Interleukin-4 ELISA Reagent Kit (Rat)
Part Number: KTE9003-2
Assay Type: ELISA (sandwich format)
Applicable Samples: Cell culture supernatants, serum, plasma, and other biological fluids

Key Application Areas

This kit is specifically designed for quantitative detection of rat IL‑4 protein and is widely used in immunology, cytokine research, and the investigation of tumor immune‑regulatory mechanisms.

Primary Applications

• ELISA applications
• Core product of cell analysis core facilities

Kit Components

• Pre‑coated microplate with rat‑derived IL‑4 capture antibody
• Rat IL‑4 protein standard
• Rat IL‑4 detection antibody
• EliKine™ Streptavidin‑HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate sealing film

Performance Specifications

Detection Range	7.5 pg/mL – 100 pg/mL
Sensitivity (Lower Limit of Detection)	5 pg/mL
Specificity	No significant cross‑reaction or background interference (validated in rat samples)

Key Points for Use

• Only reagents from the same batch may be mixed; avoid cross‑batch or expired reagent mixing.
• Allow to equilibrate at room temperature for 30 minutes before use.
• Use disposable pipette tips during assay to prevent cross‑contamination.
• Return unused plate strips to original packaging immediately and store sealed at 4 °C.
• Thorough mixing is critical for results; recommend gentle orbital shaking or manual inversion every 10 minutes.
• Set samples and standards in duplicate or triplicate wells to enhance data reliability.

Storage and Transportation

• Unopened kits should be stored at 2–8 °C (refrigerator) and protected from freeze‑thaw cycles.
• After opening, refer to the instruction manual for proper compartmentalized storage of each component.
• Transport using blue ice packs (cold chain) to ensure continuous low temperature.

Specifications and Pricing

Specification	Unit Price (CNY)	Lead Time
48 T (48 kits)	1,598.0	In stock (next‑day shipping)
96 T (96 kits)	2,558.0	In stock (next‑day shipping)

Regulatory Statement

Warning: This product is for research use only and is not intended for human clinical diagnostics. Any infringement of patents or licenses arising from the use of this product is the sole responsibility of the user.

Product Background

Interleukin‑4 (IL‑4) is a multifunctional cytokine secreted by activated T lymphocytes, mast cells, and basophils. It plays a crucial role in regulating immune responses, promoting B‑cell proliferation, and mediating antitumor activity. Quantitative detection of IL‑4 in rat models assists researchers in deciphering immune‑regulatory networks, evaluating pharmacological interventions, and conducting early‑stage investigations of novel immunotherapies.

Gene and Protein Information

• Gene ID: 287287
• Protein ID: P20096
• Aliases: IL4, BCGF‑1, BCGF1, BSF1, MGC79402, B cell growth factor 1, B_cell stimulatory factor 1, lymphocyte stimulatory factor 1
• Protein Sequence Link: UniProt - P20096

Note: The above information may change due to product optimization or upgrades; please consult YaKefin Co., Ltd. for the most current details.