Immunoglobulin G/IgG/ ELISA KI

Rat IgG ELISA Kit (One Step Method) – KTE4043-2

产品中文名称：Immunoglobulin G/IgG/ ELISA KIT 免疫球蛋白G ELISA 试剂盒（大鼠）
产品英文名称：Rat IgG ELISA Kit (One Step Method)
适用领域：细胞分析 → 蛋白类 → ELISA 应用（核心产品）

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一、产品概述

本套件为EliKine™系列的双抗体夹心法ELISA定量试剂盒，专为大鼠血清、血浆、组织匀浆、细胞上清液及其他相关液体样本中免疫球蛋白G（IgG）进行高灵敏度测定而设计。针对科研实验室的高通量需求，提供48孔和96孔两种包装规格。

二、检测原理

采用标准的“双抗体夹心法”。酶标板预包被特异性抗大鼠IgG抗体，加入样本或标准后形成抗原-捕获抗体复合物。随后加入生物素化检测试剂抗体和链霉亲和素‑HRP，形成抗体‑抗原‑抗体‑酶复合物。经洗涤后加入TMB底物，HRP催化颜色反应，终止液将蓝色转为黄色。使用酶标仪在450 nm读取OD值，OD与IgG浓度呈线性关系。

三、核心优势

• 高灵敏度：检测下限 0.15 µg/mL。
• 宽检测范围：0.75 µg/mL ~ 60 µg/mL，适用于不同浓度样本。
• 特异性强：对大鼠IgG的同类/异类蛋白交叉反应极低，背景噪声低。
• 一步法设计：简化操作流程，适合中等至高通量实验。
• 兼容性：适用于市面上常规酶标仪（450 nm）和标准实验室设备。

四、试剂盒组成

• Rat IgG Microplate
• Rat IgG Standard (120 µg/mL)
• Standard Diluent
• Rat IgG Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

五、适用样本类型

• 血清
• 血浆
• 组织匀浆
• 细胞培养上清
• 其他相关液体样本

六、实验操作要点

• 所有试剂须在使用前于室温（20‑25 ℃）平衡30 分钟。
• 采用一次性吸头，避免交叉污染。
• 未使用的板条请立即放回原包装，密封后于4 ℃保存。
• 建议使用低速振荡器或每10 分钟手动轻摇，以确保充分混匀。
• 样品和标准建议设置双孔或三孔复孔，以提升数据可靠性。
• 请勿将不同批号或超过有效期的试剂混用。

七、存储与运输

• 未开封试剂盒：避光保存于2‑8 ℃，保质期6个月。
• 启用后：请参照说明书分别保存各组分。
• 运输方式：冷链（蓝冰）包装。

八、规格与报价

包装规格	单价（CNY）	交货期
48 T	1998.00	期货（3 天内发货）
96 T	3198.00	期货（3 天内发货）

九、法规与使用声明

本产品仅限于科研用途，不适用于人类或临床诊断。使用时请遵守相关专利及许可证规定，因使用本产品可能产生的专利侵权或其他违规行为，我司不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Rat IgG ELISA Kit (One Step Method) – KTE4043-2

Product Chinese Name: Immunoglobulin G/IgG ELISA KIT (Rat)
Product English Name: Rat IgG ELISA Kit (One Step Method)
Application Area: Cell Analysis → Protein → ELISA Application (Core Product)

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1. Product Overview

This kit is part of the EliKine™ series of sandwich ELISA quantitative assays, specifically designed for high‑sensitivity detection of immunoglobulin G (IgG) in rat serum, plasma, tissue homogenates, cell culture supernatants, and other related liquid samples. To meet the high‑throughput demands of research laboratories, the kit is available in 48‑well and 96‑well plate formats.

2. Detection Principle

The assay employs the standard “sandwich” format. Microplates are precoated with a rat IgG‑specific capture antibody; upon addition of sample or standard, antigen‑capture antibody complexes form. A biotinylated detection antibody and streptavidin‑HRP are then added, creating an antibody‑antigen‑antibody‑enzyme complex. After washing, TMB substrate is introduced; HRP catalyzes a color reaction that is stopped to convert the blue color to yellow. Optical density is read at 450 nm, and the OD value is linearly proportional to IgG concentration.

3. Key Advantages

• High Sensitivity: Lower limit of detection 0.15 µg/mL.
• Broad Dynamic Range: 0.75 µg/mL ~ 60 µg/mL, suitable for samples of varying concentrations.
• Strong Specificity: Minimal cross‑reactivity with rat IgG homologous/heterologous proteins, low background noise.
• One‑Step Design: Simplified workflow, ideal for medium to high throughput experiments.
• Compatibility: Works with standard laboratory microplate readers (450 nm) and common lab equipment.

4. Kit Components

• Rat IgG Microplate
• Rat IgG Standard (120 µg/mL)
• Standard Diluent
• Rat IgG Detect Antibody
• Streptavidin‑HRP
• HRP Substrate A & B (TMB)
• Stop Solution
• Wash Buffer
• Plate Covers

5. Applicable Sample Types

• Serum
• Plasma
• Tissue Homogenates
• Cell Culture Supernatants
• Other related liquid samples

6. Critical Experimental Steps

• All reagents should be equilibrated to room temperature (20‑25 °C) for 30 minutes before use.
• Use disposable pipette tips to avoid cross‑contamination.
• Unutilized plate strips should be returned to their original packaging, sealed, and stored at 4 °C.
• Gentle agitation (low‑speed shaker) or manual rocking every 10 minutes is recommended to ensure thorough mixing.
• Run samples and standards in duplicate or triplicate wells to improve data reliability.
• Do not mix reagents from different lots or those past their expiration date.

7. Storage and Shipping

• Unopened kit: Store protected from light at 2‑8 °C; shelf life 6 months.
• After opening: Refer to the user manual for individual component storage conditions.
• Shipping method: Cold‑chain (dry ice) packaging.

8. Specifications and Pricing

Package Specification	Unit Price (CNY)	Delivery Time
48 T	1998.00	In stock (ship within 3 days)
96 T	3198.00	In stock (ship within 3 days)

9. Regulatory and Usage Statement

This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Users must comply with applicable patents and licensing agreements; the company assumes no liability for any patent infringement or regulatory violations that may arise from product use.

Note: The information above is subject to change due to product upgrades or other factors. For the most current details, please contact Yakeen.