NGFB/HSAN5/Beta-NGF/NGFB/Beta-

EliKine™ 大鼠神经生长因子 (NGF) ELISA 试剂盒 (一步法)

货号：KTE4032-2

本试剂盒采用优化的双抗体夹心一步法ELISA原理，专为精准定量检测大鼠血清、血浆、组织匀浆及细胞培养上清液等样本中的神经生长因子（NGF）水平而设计。作为细胞分析领域的核心产品，其出色的性能为神经科学、药理学及毒理学研究提供了可靠的数据支持。

产品概述

神经生长因子（NGF）是神经营养因子家族的重要成员，在神经元发育、存活、分化及损伤修复过程中扮演关键角色。本试剂盒利用高特异性的抗大鼠NGF抗体，确保检测结果的高度准确性和可靠性，有效避免与类似物的交叉反应，为您的科研数据保驾护航。

主要特性与优势

• 高灵敏度：最低检测浓度可达 3 pg/mL，能够有效检测样本中微量的NGF表达。
• 宽检测范围：检测范围为 15 pg/mL - 1200 pg/mL，满足不同浓度样本的检测需求。
• 优异特异性：经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰。
• 操作便捷：一步法操作流程简化了实验步骤，节省宝贵的研究时间。
• 即用型组分：试剂盒提供预包被微孔板及即用型标准品、缓冲液等，开盒即用，减少准备时间。

检测原理

采用双抗体夹心ELISA法。预包被于酶标板上的特异性抗体捕获样本中的大鼠NGF，随后依次与生物素化的检测抗体和链霉亲和素-HRP结合，形成免疫复合物。加入TMB底物显色后，颜色的深浅与样本中大鼠NGF的浓度成正比。

试剂盒组分

• 预包被大鼠NGF微孔板 (Rat NGF Microplate)
• 大鼠NGF标准品 (2,400 pg/mL) (Rat NGF Standard)
• 标准品稀释液 (Standard Diluent)
• 大鼠NGF检测抗体 (Rat NGF Detect Antibody)
• 链霉亲和素-HRP (Streptavidin-HRP)
• HRP底物A液 (HRP Substrate A)
• HRP底物B液 (HRP Substrate B)
• 终止液 (Stop Solution)
• 洗涤缓冲液 (Wash Buffer)
• 板盖 (Plate Covers)

规格与价格

保存与运输

保存：未启封的试剂盒请避光保存于2-8℃，有效期6个月。启用后，请参照说明书妥善保存各组分。

运输：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 实验过程中请使用一次性耗材，避免交叉污染。
• 未使用的板条需立即放回铝箔袋并密封，于4℃保存。
• 充分混匀试剂对实验结果至关重要，建议使用微量振荡器或定期手动摇匀。
• 为获得更精确的结果，建议对样品和标准品进行复孔（双孔或三孔）检测。

警告：本品仅供科研使用，不适用于人类或临床诊断。用户应确保其使用方式不违反任何专利或许可规定。

别名参考： NGFB, HSAN5, Beta-NGF, NGF-B, Beta-Nerve Growth Factor

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Rat Nerve Growth Factor (NGF) ELISA Kit (One-Step)

Catalog Number: KTE4032-2

This kit employs an optimized one-step ELISA principle based on the double-antibody sandwich method. It is specifically designed for the accurate quantitative detection of Nerve Growth Factor (NGF) levels in samples such as rat serum, plasma, tissue homogenates, and cell culture supernatants. As a core product in cell analysis, its excellent performance provides reliable data support for neuroscience, pharmacology, and toxicology research.

Product Overview

Nerve Growth Factor (NGF) is a key member of the neurotrophin family and plays a critical role in neuronal development, survival, differentiation, and damage repair. This kit utilizes highly specific anti-rat NGF antibodies to ensure highly accurate and reliable detection results, effectively avoiding cross-reactivity with analogs and safeguarding your research data.

Key Features & Advantages

• High Sensitivity: The minimum detectable concentration can reach 3 pg/mL, enabling effective detection of low-level NGF expression in samples.
• Wide Detection Range: The detection range is 15 pg/mL - 1200 pg/mL, meeting the detection needs for samples of varying concentrations.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity or background interference with target protein analogs.
• Convenient Operation: The one-step procedure simplifies the experimental process, saving valuable research time.
• Ready-to-Use Components: The kit provides pre-coated microplates and ready-to-use standards, buffers, etc., allowing for immediate use and reduced preparation time.

Detection Principle

Utilizes the double-antibody sandwich ELISA method. Specific antibodies pre-coated on the microplate capture rat NGF from the sample. This is followed by sequential binding with a biotinylated detection antibody and Streptavidin-HRP, forming an immune complex. After adding TMB substrate for color development, the intensity of the color is proportional to the concentration of rat NGF in the sample.

Kit Components

• Pre-coated Rat NGF Microplate
• Rat NGF Standard (2,400 pg/mL)
• Standard Diluent
• Rat NGF Detect Antibody
• Streptavidin-HRP
• HRP Substrate A
• HRP Substrate B
• Stop Solution
• Wash Buffer
• Plate Covers

Specifications & Pricing

Storage & Shipping

Storage: Store the unopened kit protected from light at 2-8°C; it is stable for 6 months. After opening, please store the components properly according to the instructions.

Shipping: The entire shipment is conducted at low temperatures using ice packs (blue ice) to ensure reagent activity.

Important Notes

• Do not mix reagents from different lot numbers or use expired reagents.
• Allow all reagents to equilibrate to room temperature for approximately 30 minutes before use.
• Use disposable consumables during the experiment to avoid cross-contamination.
• Immediately return unused strips to the foil pouch and seal it for storage at 4°C.
• Thorough mixing of reagents is crucial for experimental results; using a microplate shaker or regular manual shaking is recommended.
• For more accurate results, it is recommended to test samples and standards in duplicate or triplicate.

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. Users should ensure their use does not violate any patent or licensing regulations.

Alias Reference: NGFB, HSAN5, Beta-NGF, NGF-B, Beta-Nerve Growth Factor

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.