大鼠趋化因子1 ELISA定量试剂盒(CXCL1)

产品概述

EliKine™ 大鼠趋化因子1 (CXCL1) ELISA定量试剂盒是一款高灵敏度的双抗体夹心法酶联免疫吸附检测试剂盒，专门设计用于定量检测大鼠血清、血浆或细胞培养上清样本中的CXCL1浓度。该试剂盒是细胞分析领域的核心产品，具有卓越的特异性，可有效避免交叉反应或背景干扰，为科研实验提供可靠数据支持。

核心特性

• 高灵敏度检测：最低检测限可达15.63 pg/mL，确保低浓度样本的精准定量。
• 宽线性范围：检测范围为15.63 pg/mL至1,000 pg/mL，覆盖多种样本浓度需求。
• 优异特异性：经严格验证，对目标蛋白类似物无明显交叉反应，结果可靠。
• 即用型设计：预包被微孔板与优化试剂组分，简化操作流程，提升实验效率。

试剂盒组分

组分名称	规格
预包被Rat CXCL1微孔板	1板
Rat CXCL1标准品（冻干）	1支
标准品稀释液	1瓶
Assay Buffer (10×)	1瓶
Rat CXCL1检测抗体 (100×)	1瓶
链霉亲和素-HRP (100×)	1瓶
HRP底物（TMB）	1瓶
终止液	1瓶
洗涤缓冲液 (20×)	1瓶
板盖	若干

应用背景

趋化因子CXCL1属于CXC趋化因子家族，在血管生长、炎症反应、伤口愈合及肿瘤发生等生理病理过程中发挥关键作用。本品通过特异性结合大鼠CXCL1，助力研究者深入探索其在大鼠模型中的功能机制。

保存与运输

• 保存条件：未开封试剂盒避光保存于2-8℃，有效期24个月；启用后请参照说明书保存各组分。
• 运输方式：冰袋运输（蓝冰），确保试剂活性。

注意事项

• 请勿混用不同批号或过期试剂。
• 使用前室温平衡30分钟，避免交叉污染。
• 推荐使用复孔检测（双孔或三孔），以确保结果准确性。
• 实验过程中需充分混匀反应体系，建议使用振荡器或定期手动摇匀。

重要提示：本产品仅供研究使用，不适用于人类或临床诊断。用户应确保使用过程符合相关法规及知识产权要求。

产品规格与价格

规格	价格（元）	货期
48 T	1598.0	请咨询
96 T	2558.0	请咨询

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

EliKine™ Rat Chemokine (CXCL1) ELISA Quantitation Kit is a high‑sensitivity sandwich ELISA kit specifically designed for the quantitative determination of CXCL1 concentrations in rat serum, plasma, or cell culture supernatants. This core product in cellular analysis offers outstanding specificity, effectively eliminating cross‑reactivity and background interference, thereby providing reliable data for research experiments.

Key Features

• High‑sensitivity detection: Lower limit of detection of 15.63 pg/mL, ensuring accurate quantitation of low‑abundance samples.
• Wide linear range: Quantification range from 15.63 pg/mL to 1,000 pg/mL, accommodating diverse sample concentrations.
• Excellent specificity: Rigorously validated with negligible cross‑reactivity toward related proteins, delivering trustworthy results.
• Ready‑to‑use format: Pre‑coated microplate and optimized reagent components streamline workflow and increase laboratory throughput.

Kit Components

Component	Specification
Pre‑coated Rat CXCL1 Microplate	1 plate
Rat CXCL1 Standard (lyophilized)	1 vial
Standard Diluent	1 bottle
Assay Buffer (10×)	1 bottle
Rat CXCL1 Detection Antibody (100×)	1 bottle
Streptavidin‑HRP (100×)	1 bottle
HRP Substrate (TMB)	1 bottle
Stop Solution	1 bottle
Wash Buffer (20×)	1 bottle
Plate Seal	multiple

Application Background

The chemokine CXCL1 belongs to the CXC family and plays pivotal roles in angiogenesis, inflammation, wound healing, and tumorigenesis. This kit specifically binds rat CXCL1, enabling researchers to dissect its functional mechanisms in rodent models.

Storage and Shipping

• Storage conditions: Store unopened kit protected from light at 2‑8 °C; shelf life 24 months. After opening, follow the instruction manual for component‑specific storage.
• Shipping method: Shipped on ice packs (blue ice) to preserve reagent activity.

Precautions

• Do not mix different lot numbers or expired reagents.
• Allow reagents to equilibrate to room temperature for 30 minutes before use; avoid cross‑contamination.
• Use duplicate or triplicate wells to ensure data reliability.
• Mix reaction mixtures thoroughly during the assay; use a plate shaker or manually invert periodically.

Important Note: This product is intended for research use only and is not suitable for human or clinical diagnostic applications. Users must ensure compliance with applicable regulations and intellectual‑property requirements.

Product Specifications and Pricing

Specification	Price (CNY)	Lead Time
48‑T	1598.0	Contact for details
96‑T	2558.0	Contact for details

Note: The information above is subject to change due to product optimization or other factors; please contact Yakein for the most current details.