抗Erk 1/2 (Phospho Thr202/Tyr20
4)单抗 | Anti-Erk 1/2 (Phospho Thr202/Tyr204) Monoclonal Antibody- 询价
- Nebulabio
- 中国
- NBAB-191482
- 2026年01月05日
- ELISA, IP, IF, IHC, WB
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant Human Erk 1/2 (Phospho Thr202/Tyr204) protein
- 形态：
liquid
- 克隆性：
单克隆
- 保质期：
见COA
- 库存：
大量
- 供应商：
北京辰辉创聚生物技术有限公司
- 应用范围：
ELISA, IP, IF, IHC, WB
- 靶点：
Erk 1/2 (Phospho Thr202/Tyr204)
- 抗体英文名：
Anti-Erk 1/2 (Phospho Thr202/Tyr204) Monoclonal Antibody
- 抗体名：
抗Erk 1/2 (Phospho Thr202/Tyr204)单抗
- 规格：
100ul; 500ul; 1ml
| 产品信息
品名：NebuSelect™ Anti-Erk 1/2 (Phospho Thr202/Tyr204) Monoclonal Antibody
别名：MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p
货号：NBAB-191482
品牌：Nebulabio
规格：100ul; 500ul; 1ml
| 产品描述
NebuSelect™ Anti-Erk 1/2 (Phospho Thr202/Tyr204) Monoclonal Antibody (Cat# NBAB-191482) was produced in rabbits immunized with recombinant Human Erk 1/2 (Phospho Thr202/Tyr204) protein, and it reacts with Human, Mouse, Rat. This antibody is validated for ELISA, IP, IF, IHC, WB.
| 产品属性
Product Category：Rabbit MAb
Reactivity：Human, Mouse, Rat
Specificity：Human Erk 1/2 (Phospho Thr202/Tyr204)
Immunogen：Recombinant Human Erk 1/2 (Phospho Thr202/Tyr204) protein
Isotype：Monoclonal Rabbit IgG
Purification：Protein A & Protein G Affinity Purification
Buffer：0.2 μm filtered solution in PBS, pH7.4, containing 0.09% Sodium azide, 50% glycerol.
Conjugate：Unconjugated
Applications：ELISA, IP, IF, IHC, WB
Dilution：ELISA 1:2000～1:10000; IP:1:50～1:500 IF 1:50～1:500; IHC-P 1:50～1:500; WB: 1:1000～1:5000
Storage：This liquid antibody can be stored at 2℃-8℃ for at least two weeks. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or antibody diluent, the antibody is stable for at least two weeks at 2-4℃.
For Research Use Only!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
, which may be used to specifically detect Ser, Thr, Ser/Thr, or Tyr residues phosphorylated by different protein kinase families. These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase?induced substrate
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
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