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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 检测范围:
15.63 pg/mL-1,000 pg/mL
- 检测方法:
夹心法
- 应用:
ELISA检测
- 适应物种:
人
- 标记物:
DPPIV/CD26
- 样本:
细胞培养上清#血清#血浆
- 灵敏度:
15.63 pg/mL
- 规格:
48 T/96 T
| 规格: | 48 T | 产品价格: | ¥1598.0 |
|---|---|---|---|
| 规格: | 96 T | 产品价格: | ¥2558.0 |
Human DPPIV/CD26 ELISA Kit (人DPPIV/CD26 ELISA试剂盒)
货号: KTE3085-2
产品概述
本试剂盒为EliKine™系列核心产品,专门用于定量检测人样本(包括细胞培养上清、血清、血浆)中的二肽基多肽酶IV (DPPIV/CD26) 含量。采用高灵敏度的双抗体夹心ELISA原理,确保检测结果精准可靠。
产品特点与优势
- 高灵敏度:最低检测浓度可达15.63 pg/mL。
- 优异特异性:经过严格验证,对目标蛋白类似物无明显交叉反应或背景干扰。
- 宽检测范围:15.63 pg/mL - 1,000 pg/mL,满足不同浓度样本的检测需求。
- 操作便捷:试剂盒提供完整组分,优化实验流程,节省准备时间。
试剂盒组分
- Human DPPIV Microplate
- Human DPPIV Standard (冻干)
- Assay Buffer (10×)
- Human DPPIV Detect Antibody (100×)
- Streptavidin-HRP (100×)
- HRP Substrate (TMB)
- Stop Solution
- Wash Buffer (20×)
- Plate Covers
背景信息
二肽基多肽酶IV (DPPIV/CD26) 是一种广泛表达于细胞表面的抗原酶,参与免疫调节、信号转导和细胞凋亡等关键生物过程。作为丝氨酸外肽酶,DPPIV在葡萄糖代谢中发挥重要作用,并涉及肠促胰岛素(如GLP-1)的降解。近年研究发现,DPPIV与肿瘤发展、器官纤维化及病毒感染密切相关,是多种疾病研究中的重要生物标志物。
技术参数
| 检测方法 | 夹心法ELISA |
|---|---|
| 反应性 | 人 |
| 样本类型 | 细胞培养上清、血清、血浆 |
| 检测范围 | 15.63 - 1,000 pg/mL |
| 灵敏度 | 15.63 pg/mL |
保存与运输
未开封试剂盒请避光保存于2-8℃,有效期24个月。开封后请参照说明书保存各组分。产品采用冰袋(蓝冰)运输,以确保试剂稳定性。
注意事项
- 请勿混合使用不同批号或过期试剂。
- 使用前所有试剂需室温平衡30分钟。
- 实验过程中请使用一次性吸头,避免交叉污染。
- 未使用的板条应立即密封,4℃保存。
- 推荐使用低速振荡器或定期手动摇匀,确保充分混匀。
- 为提高准确性,建议对样品和标准品进行双孔或三孔检测(复孔)。
产品规格与价格
| 规格 | 价格 (元) | 货期 |
|---|---|---|
| 48 T | 1598.0 | 请咨询 |
| 96 T | 2558.0 | 请咨询 |
警告:本产品仅供研究使用,不适用于人类或临床诊断。使用前请确保符合相关法规及许可要求。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Human DPPIV/CD26 ELISA Kit
Catalog Number: KTE3085-2
Product Overview
This kit is a core product of the EliKine™ series, specifically designed for the quantitative detection of Dipeptidyl Peptidase IV (DPPIV/CD26) levels in human samples, including cell culture supernatants, serum, and plasma. Utilizing the highly sensitive sandwich ELISA principle, it ensures accurate and reliable results.
Features & Advantages
- High Sensitivity: Minimum detectable concentration reaches 15.63 pg/mL.
- Excellent Specificity: Rigorously validated, showing no significant cross-reactivity or background interference with analogous proteins.
- Wide Detection Range: 15.63 pg/mL - 1,000 pg/mL, suitable for samples with varying concentrations.
- Convenient Operation: The kit provides complete components with an optimized protocol, saving preparation time.
Kit Components
- Human DPPIV Microplate
- Human DPPIV Standard (Lyophilized)
- Assay Buffer (10×)
- Human DPPIV Detect Antibody (100×)
- Streptavidin-HRP (100×)
- HRP Substrate (TMB)
- Stop Solution
- Wash Buffer (20×)
- Plate Covers
Background Information
Dipeptidyl Peptidase IV (DPPIV/CD26) is a widely expressed cell surface antigenic enzyme involved in key biological processes such as immune regulation, signal transduction, and apoptosis. As a serine exopeptidase, DPPIV plays a significant role in glucose metabolism and is involved in the degradation of incretins (e.g., GLP-1). Recent studies indicate that DPPIV is closely associated with tumor development, organ fibrosis, and viral infections (such as MERS-CoV), making it an important biomarker in research for various diseases.
Technical Parameters
| Assay Method | Sandwich ELISA |
|---|---|
| Reactivity | Human |
| Sample Type | Cell Culture Supernatant, Serum, Plasma |
| Detection Range | 15.63 - 1,000 pg/mL |
| Sensitivity | 15.63 pg/mL |
Storage and Shipping
The unopened kit should be stored protected from light at 2-8°C and is stable for 24 months. After opening, please refer to the instructions for storage of individual components. The product is shipped with ice packs (blue ice) to ensure reagent stability.
Precautions
- Do not mix reagents from different lots or use expired reagents.
- All reagents should be equilibrated to room temperature for 30 minutes before use.
- Use disposable pipette tips throughout the procedure to avoid cross-contamination.
- Unused strips should be resealed immediately and stored at 4°C.
- Use a low-speed shaker or periodic manual shaking to ensure thorough mixing.
- For improved accuracy, it is recommended to assay samples and standards in duplicate or triplicate.
Specifications & Pricing
| Specification | Price (CNY) | Availability |
|---|---|---|
| 48 T | 1598.0 | Please inquire |
| 96 T | 2558.0 | Please inquire |
Warning: This product is for research use only. Not for use in humans or clinical diagnosis. Ensure compliance with relevant regulations and licensing requirements before use.
Note: The above information is subject to change due to product optimization and upgrades. Please consult Ya Keyin Company for specific details.
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文献和实验a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
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