血管内皮生长因子酶联免疫试剂盒(人) VEGF ELISA

Human VEGF ELISA Kit (人血管内皮生长因子酶联免疫试剂盒)

货号: KTE6033-3 | 产品类型: 夹心法ELISA核心试剂盒

产品概述

本试剂盒采用双抗体夹心ELISA原理，专为精准定量检测人血清、血浆、细胞培养上清及其他生物液体样本中的血管内皮生长因子(VEGF)蛋白水平而设计。基于EliKine™技术平台，该试剂盒在细胞分析及蛋白功能研究中展现出卓越的可靠性和重复性，是肿瘤生物学、血管生成研究及代谢疾病研究领域的理想工具。

核心特性与优势

• 高灵敏度：最低检测浓度达16 pg/mL，可准确识别低丰度样本
• 宽线性范围：检测范围覆盖31.25 pg/mL至2000 pg/mL，满足不同浓度样本需求
• 优异特异性：经严格验证，与人VEGF-A蛋白特异性结合，无明显交叉反应
• 即用型设计：预包被微孔板与优化试剂配方，简化操作流程
• 批次稳定性：严格质控确保不同批次间结果一致性

试剂盒组分

• 人VEGF-A抗体预包被微孔板（可拆卸）
• 重组人VEGF-A蛋白标准品（浓度梯度预稀释）
• 生物素标记检测抗体
• EliKine™链霉亲和素-HRP结合物
• 标准品稀释液、实验缓冲液、洗涤缓冲液
• TMB底物溶液 & 反应终止液
• 封板膜 & 详细说明书

实验参数

样本类型	细胞培养上清、血清、血浆、其他生物液体
反应性	人（Homo sapiens）
检测时间	约3.5小时（含孵育与显色步骤）
推荐复孔	双孔或三孔检测以确保数据准确性

背景信息

血管内皮生长因子(VEGF)是调控血管生成的关键因子，通过与其受体结合促进内皮细胞增殖、迁移及血管通透性增加。其表达水平与肿瘤转移、糖尿病视网膜病变、慢性炎症等多种病理过程密切相关。人VEGF存在VEGF₁₂₁、VEGF₁₆₅等多种亚型，其中VEGF₁₆₅为最常见活性形式。

保存与运输

• 保存条件：未开封试剂盒2-8℃保存；开封后请按组分要求分别储存
• 运输方式：蓝冰低温运输，确保试剂活性
• 稳定性：有效期见标签，避免反复冻融

订购信息

规格	价格（人民币）	货期
48 T（可检测42个样本）	1,598.0	现货，次日发货
96 T（可检测90个样本）	2,558.0	现货，次日发货

备注：本产品仅供科研使用，不适用于临床诊断。操作前请详细阅读说明书并遵循实验室生物安全规范。

更多技术细节请参考UniProt数据库编号：P15692

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human VEGF ELISA Kit

Catalog Number: KTE6033-3 | Assay Type: Sandwich ELISA Core Kit

Product Overview

This kit employs a double-antibody sandwich ELISA principle and is specifically designed for the precise quantitative detection of Vascular Endothelial Growth Factor (VEGF) protein levels in human serum, plasma, cell culture supernatants, and other biological fluid samples. Based on the EliKine™ technology platform, this kit demonstrates exceptional reliability and reproducibility in cell analysis and protein function studies, making it an ideal tool for tumor biology, angiogenesis research, and metabolic disease research.

Key Features & Advantages

• High Sensitivity: Minimum detectable concentration reaches 16 pg/mL, enabling accurate identification of low-abundance samples.
• Wide Linear Range: Detection range covers 31.25 pg/mL to 2000 pg/mL, meeting the needs of samples with varying concentrations.
• Excellent Specificity: Rigorously validated for specific binding to human VEGF-A protein with no significant cross-reactivity.
• Ready-to-Use Design: Pre-coated microplate and optimized reagent formulations simplify the operation procedure.
• Lot Consistency: Strict quality control ensures consistent results across different lots.

Kit Components

• Pre-coated Microplate (removable) with Human VEGF-A Antibody
• Recombinant Human VEGF-A Protein Standard (pre-diluted in concentration gradients)
• Biotin-labeled Detection Antibody
• EliKine™ Streptavidin-HRP Conjugate
• Standard Diluent, Assay Buffer, Wash Buffer
• TMB Substrate Solution & Stop Solution
• Plate Sealer & Detailed Instruction Manual

Assay Parameters

Sample Type	Cell culture supernatant, serum, plasma, other biological fluids
Reactivity	Human (Homo sapiens)
Assay Time	Approximately 3.5 hours (including incubation and color development steps)
Recommended Replicates	Duplicate or triplicate wells to ensure data accuracy

Background Information

Vascular Endothelial Growth Factor (VEGF) is a key regulator of angiogenesis, promoting endothelial cell proliferation, migration, and increased vascular permeability through binding to its receptors. Its expression levels are closely associated with various pathological processes such as tumor metastasis, diabetic retinopathy, and chronic inflammation. Human VEGF exists in multiple isoforms including VEGF₁₂₁ and VEGF₁₆₅, with VEGF₁₆₅ being the most common active form.

Storage & Shipping

• Storage Conditions: Unopened kit should be stored at 2-8°C; after opening, store components separately as required.
• Shipping Method: Shipped on blue ice to ensure reagent activity.
• Stability: Refer to the expiration date on the label; avoid repeated freeze-thaw cycles.

Ordering Information

Size	Price (RMB)	Lead Time
48 T (for 42 samples)	1,598.0	In stock, ships next day
96 T (for 90 samples)	2,558.0	In stock, ships next day

Note: This product is for research use only, not for clinical diagnosis. Please read the manual carefully and follow laboratory biosafety regulations before operation.

For more technical details, please refer to UniProt database accession number: P15692

Note: The above information is subject to change due to product optimization and upgrades. Please contact Yaken Company for specific details.