白介素2受体酶联免疫试剂盒(人) sIL-2Rα ELISA

EliKine™ 人白介素2受体α(sIL-2Rα/CD25) ELISA试剂盒

货号: KTE3046-3 | 产品分类: 核心产品 | 反应性: 人

产品概述

本试剂盒采用双抗体夹心ELISA原理，专门用于定量检测人细胞培养上清、血清或血浆样本中的可溶性白介素2受体α(sIL-2Rα/CD25)。作为细胞分析领域的核心工具，该试剂盒为免疫学研究、疾病机制探索及生物标志物分析提供了可靠的技术支持。

关键特性

• 高灵敏度: 最低检测限达5.76 pg/mL，可精准检测低浓度样本
• 宽检测范围: 78.13-5,000 pg/mL的线性范围，满足不同浓度样本需求
• 优异特异性: EliKine™技术确保对目标蛋白的高特异性识别，无明显交叉反应
• 标准化操作: 优化的缓冲体系和完善的组分配置，确保实验重复性

试剂盒组分

组分名称	规格	状态
Human sIL-2Rα预包被微孔板	48T/96T	即用型
Human sIL-2Rα标准品(冻干)	2支	使用前重建
标准品稀释液	15mL/30mL	即用型
检测抗体(100×浓缩液)	0.1mL/0.2mL	使用前稀释
链霉亲和素-HRP(100×)	0.1mL/0.2mL	使用前稀释
TMB显色底物	12mL/24mL	避光保存
终止液	12mL/24mL	即用型
洗涤缓冲液(20×浓缩液)	25mL/50mL	使用前稀释
板盖	2个/4个	即用型

技术参数

• 样本类型: 细胞培养上清、血清、血浆
• 检测方法: 夹心法ELISA
• 孵育时间: 标准流程约3.5小时
• 检测波长: 450nm主波长，620nm参考波长

背景说明

可溶性IL-2受体α(sIL-2Rα)是T细胞和B细胞活化的重要标志物。其水平变化与多种免疫相关疾病密切相关，包括类风湿性关节炎、系统性红斑狼疮、白血病和淋巴瘤等。监测sIL-2Rα浓度可为疾病进程评估和治疗效果监测提供有价值的参考数据。

保存与运输

• 保存条件: 未开封试剂盒避光保存于2-8℃，有效期24个月
• 运输条件: 全程蓝冰冰袋运输，确保试剂稳定性

订购信息

规格	价格(元)	货期
48T	1598.0	期货(5个工作日内发货)
96T	2558.0	期货(5个工作日内发货)

重要提示: 本产品仅用于科学研究，不适用于临床诊断。实验操作前请仔细阅读说明书，严格按照操作规范进行。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

EliKine™ Human Interleukin-2 Receptor α (sIL-2Rα/CD25) ELISA Kit

Product No.: KTE3046-3 | Product Category: Core Product | Species Reactivity: Human

Product Overview

This kit utilizes a sandwich ELISA format with capture and detection antibodies, specifically designed for quantitative measurement of soluble interleukin‑2 receptor α (sIL‑2Rα/CD25) in human cell culture supernatants, serum, or plasma samples. As a core tool in the cell analysis field, the kit provides reliable technical support for immunology research, disease‑mechanism investigations, and biomarker analyses.

Key Features

• High Sensitivity: Lower limit of detection of 5.76 pg/mL, enabling accurate detection of low‑abundance samples
• Broad Dynamic Range: Linear range of 78.13–5,000 pg/mL, suitable for a variety of sample concentrations
• Excellent Specificity: EliKine™ technology ensures high specificity for the target protein with negligible cross‑reactivity
• Standardized Workflow: Optimized buffer system and well‑defined component configuration ensure assay reproducibility

Kit Components

Component	Specification	Status
Human sIL‑2Rα Pre‑coated Microplate	48T/96T	Ready‑to‑use
Human sIL‑2Rα Standard (lyophilized)	2 vials	Reconstitute before use
Standard Diluent	15 mL/30 mL	Ready‑to‑use
Detection Antibody (100× concentrate)	0.1 mL/0.2 mL	Dilute before use
Streptavidin‑HRP (100×)	0.1 mL/0.2 mL	Dilute before use
TMB Substrate	12 mL/24 mL	Protect from light
Stop Solution	12 mL/24 mL	Ready‑to‑use
Wash Buffer (20× concentrate)	25 mL/50 mL	Dilute before use
Plate Seal	2 pcs/4 pcs	Ready‑to‑use

Technical Specifications

• Sample Types: Cell culture supernatants, serum, plasma
• Assay Method: Sandwich ELISA
• Incubation Time: Approximately 3.5 h following the standard protocol
• Reading Wavelength: 450 nm primary, 620 nm reference

Background

Soluble IL‑2 receptor α (sIL‑2Rα) is an important marker of T‑cell and B‑cell activation. Alterations in its level are closely associated with various immune‑mediated diseases, including rheumatoid arthritis, systemic lupus erythematosus, leukemia, and lymphoma. Monitoring sIL‑2Rα concentrations can provide valuable information for disease progression assessment and therapeutic response evaluation.

Storage and Shipping

• Storage Conditions: Unopened kits should be stored protected from light at 2–8 °C; shelf life 24 months
• Shipping Conditions: Transported on dry ice packs to maintain reagent stability

Ordering Information

Specification	Price (CNY)	Lead Time
48T	1598.0	Future stock (dispatch within 5 working days)
96T	2558.0	Future stock (dispatch within 5 working days)

Important Notice: This product is intended for scientific research only and is not for clinical diagnostics. Please read the manual carefully and follow the protocol strictly before conducting any experiments.

Note: The above information may be subject to change due to product optimization or upgrades; please contact Yakein® for the latest details.