MMP-9 ELISA KIT 基质金属蛋白酶-9ELISA

使用本试剂盒时请遵守所在机构的生物安全与知识产权规定；如因专利或许可证问题导致的侵权风险，责任由使用者自行承担。

背景信息（MMP‑9）

基质金属蛋白酶‑9（MMP‑9，Gene ID: 4318，Uniprot P14780），亦称92 kDa IV型胶原酶、明胶酶B，属于锌‑金属蛋白酶家族。该酶介导细胞外基质降解，参与胚胎发育、血管生成、伤口愈合、细胞迁移等生理过程，同时在关节炎、脑出血及肿瘤转移等病理状态中发挥关键作用。

检索关键词

基质金属蛋白酶-9 ELISA试剂盒、MMP‑9、Human MMP‑9 ELISA Kit、KTE6027、Matrix Metalloproteinase‑9、Gelatinase B、ELISA 核心产品

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human MMP‑9 ELISA Kit (KTE6027‑2)

Product Overview

Product Name: MMP‑9 ELISA KIT, Matrix Metalloproteinase‑9 ELISA Kit (Human)
Catalog Number: KTE6027‑2
Assay Type: ELISA (Sandwich)
Suitable Samples: Cell culture supernatants, serum, plasma, and other biological liquids

Applications

• Quantitative analysis of Matrix Metalloproteinase‑9 (MMP‑9) at the cellular level
• Core ELISA product supporting the development and quality control of cell analysis platforms
• Suitable for basic research, drug mechanism studies, and biomarker screening

Assay Principle

This kit is based on the sandwich ELISA technique: Microplates pre-coated with a human MMP‑9 capture antibody capture MMP‑9 from samples or standards. Subsequently, a human detection antibody, EliKine™ Streptavidin‑HRP, and HRP substrate are added. Quantification is achieved by measuring the absorbance at 450 nm via a colorimetric reaction.

Key Performance Indicators

• Detection Range: 31.25 pg/mL – 2,000 pg/mL
• Sensitivity (Lower Limit of Detection): 16 pg/mL
• High Specificity: No significant cross-reactivity or background interference detected
• Conforms to standard ELISA laboratory operating procedures

Kit Components

• Microplate pre-coated with Human MMP‑9 Antibody
• Human MMP‑9 Protein Standard (gradient concentrations)
• Human MMP‑9 Detection Antibody
• EliKine™ Streptavidin‑HRP
• Standard Diluent
• Assay Buffer (for washing, dilution, blocking)
• HRP Substrate
• Stop Solution
• Wash Buffer
• Plate Sealer

Usage Guide (Key Points)

1. Equilibrate all reagents to room temperature for 30 minutes before use.
2. Use disposable pipette tips to prevent cross-contamination.
3. Immediately return unused strips to the packaging, seal, and store at 4 ℃.
4. Critical mixing step: Use a low-speed shaker or manually shake gently every 10 minutes.
5. It is recommended to assay samples and standards in duplicate or triplicate to improve data reliability.
6. Do not mix reagents from different lots or expired reagents.

Storage and Shipping

• Unopened Kit: Store at 2 °C – 8 °C.
• After opening, store components separately according to the instructions.
• Shipping Method: Shipped with ice packs (blue ice) under cold chain conditions to ensure temperature control throughout transit.

Specifications and Ordering

Regulatory and Safety Notes

This product is intended for Research Use Only (RUO) and is not intended for human clinical diagnosis or treatment.

Please adhere to your institution's biosafety and intellectual property regulations when using this kit; users assume full responsibility for any infringement risks arising from patent or licensing issues.

Background Information (MMP‑9)

Matrix Metalloproteinase‑9 (MMP‑9, Gene ID: 4318, Uniprot P14780), also known as 92 kDa type IV collagenase or Gelatinase B, belongs to the zinc-metalloproteinase family. This enzyme mediates extracellular matrix degradation and is involved in physiological processes such as embryonic development, angiogenesis, wound healing, and cell migration. It also plays a key role in pathological conditions such as arthritis, cerebral hemorrhage, and tumor metastasis.

Search Keywords

Matrix Metalloproteinase-9 ELISA Kit, MMP‑9, Human MMP‑9 ELISA Kit, KTE6027, Matrix Metalloproteinase‑9, Gelatinase B, Core ELISA Product

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.