Adiponectin/Acrp30 ELISA KIT 脂

Human Adiponectin ELISA Kit (KTE3147-2)

产品概述

本试剂盒是一款基于双抗体夹心法原理，专门用于定量检测人血清、血浆及细胞培养上清中脂联素(Adiponectin/Acrp30)水平的核心ELISA试剂盒。作为EliKine™系列产品的一员，其设计旨在为代谢研究领域提供高价值的分析工具。

主要特性

• 高灵敏度：最低检测限可达62.5 pg/mL
• 宽检测范围：62.5 pg/mL - 4,000 pg/mL，适用于不同浓度水平的样本检测。
• 优异特异性：经严格验证，与目标蛋白类似物无明显交叉反应或背景干扰。
• 即用型组分：提供预包被板条及优化的缓冲液体系，简化操作流程。

产品应用

该试剂盒适用于研究脂联素在葡萄糖调节、脂肪酸氧化等代谢过程中的作用，以及对2型糖尿病、肥胖、动脉粥样硬化、非酒精性脂肪肝等疾病的相关机制探索。

试剂盒组分（详细清单）

• 预包被微孔板 (Human Adiponectin Microplate)
• 脂联素标准品 (冻干) (Human Adiponectin Standard, lyophilized)
• 10倍浓缩检测缓冲液 (Assay Buffer, 10×)
• 100倍浓缩生物素化检测抗体 (Human Adiponectin Detect Antibody, 100×)
• 100倍浓缩链霉亲和素-HRP (Streptavidin-HRP, 100×)
• HRP底物 (TMB)
• 终止液 (Stop Solution)
• 20倍浓缩洗涤缓冲液 (Wash Buffer, 20×)
• 板盖 (Plate Covers)

技术参数

反应性	人 (Human)
样本类型	细胞培养上清、血清、血浆
检测方法	夹心法ELISA
规格与价格	48 T: ￥1598.00 96 T: ￥2558.00 货期请咨询。

操作与保存

保存建议：未开封试剂盒请避光保存于2-8℃，保质期24个月。启用后，请参照说明书妥善保存各组分。

运输条件：全程冰袋（蓝冰）运输，确保试剂活性。

重要注意事项

• 请勿混合使用不同批号或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请务必使用一次性吸头。
• 未使用的板条需立即放回原包装并密封，于4℃保存。
• 为保证反应充分，建议在反应过程中使用低速振荡器或定期手动摇匀。
• 为获得更可靠的结果，推荐对样品和标准品进行复孔（双孔或三孔）检测。

背景信息

脂联素（Adiponectin/Acrp30）是一种由脂肪组织特异性分泌的蛋白质激素，在调节葡萄糖稳定和脂肪酸分解等代谢过程中扮演关键角色。其可形成三聚体、六聚体及高阶寡聚体，不同形式的生物活性可能存在差异。研究表明，脂联素具有抗动脉粥样硬化及抗炎潜力，其血清水平变化与多种代谢性疾病密切相关。

警告

本产品仅限用于科学研究，不适用于人类或临床诊断。用户有责任确保其使用方式不违反任何专利或许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Adiponectin ELISA Kit (KTE3147-2)

Product Overview

This kit is a core ELISA kit based on the double-antibody sandwich principle, specifically designed for the quantitative detection of Adiponectin/Acrp30 levels in human serum, plasma, and cell culture supernatants. As a member of the EliKine™ series, it is designed to provide a high-value analytical tool for the field of metabolic research.

Key Features

• High Sensitivity: Minimum detection limit as low as 62.5 pg/mL.
• Broad Detection Range: 62.5 pg/mL - 4,000 pg/mL, suitable for samples with varying concentration levels.
• Excellent Specificity: Rigorously validated, showing no significant cross-reactivity or background interference with target protein analogs.
• Ready-to-Use Components: Includes pre-coated strips and an optimized buffer system to streamline the operation.

Product Applications

This kit is suitable for studying the role of adiponectin in metabolic processes such as glucose regulation and fatty acid oxidation, as well as for exploring mechanisms related to diseases such as type 2 diabetes, obesity, atherosclerosis, and non-alcoholic fatty liver disease.

Kit Components (Detailed List)

• Pre-coated Microplate (Human Adiponectin Microplate)
• Adiponectin Standard (lyophilized) (Human Adiponectin Standard, lyophilized)
• 10X Concentrated Assay Buffer (Assay Buffer, 10×)
• 100X Concentrated Biotinylated Detection Antibody (Human Adiponectin Detect Antibody, 100×)
• 100X Concentrated Streptavidin-HRP (Streptavidin-HRP, 100×)
• HRP Substrate (TMB)
• Stop Solution
• 20X Concentrated Wash Buffer (Wash Buffer, 20×)
• Plate Covers

Technical Parameters

Reactivity	Human
Sample Type	Cell culture supernatant, serum, plasma
Detection Method	Sandwich ELISA
Specifications & Pricing	48 T: ￥1598.00 96 T: ￥2558.00 Please inquire for delivery time.

Operation & Storage

Storage Recommendation: Store unopened kit protected from light at 2-8℃; shelf life is 24 months. After opening, store individual components properly according to the instructions.

Shipping Conditions: Shipped with ice packs (blue ice) throughout to ensure reagent activity.

Important Notes

• Do not mix reagents from different lots or those that have expired.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• To avoid cross-contamination, always use disposable pipette tips during the experiment.
• Unused strips should be immediately returned to the original packaging, sealed, and stored at 4°C.
• To ensure sufficient reaction, it is recommended to use a low-speed shaker or perform periodic manual shaking during the incubation steps.
• For more reliable results, it is recommended to perform replicate (duplicate or triplicate) measurements for both samples and standards.

Background Information

Adiponectin (Adiponectin/Acrp30) is a protein hormone specifically secreted by adipose tissue and plays a key role in regulating metabolic processes such as glucose homeostasis and fatty acid catabolism. It can form trimers, hexamers, and higher-order oligomers, and the biological activity may vary among different forms. Research indicates that adiponectin has anti-atherosclerotic and anti-inflammatory potential, and changes in its serum levels are closely associated with various metabolic diseases.

Warning

This product is for research use only and is not intended for human or clinical diagnostic use. The user is responsible for ensuring that their use does not infringe any patents or license agreements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.