TWEAK ELISA KIT 相关弱凋亡诱导因子ELISA

Human TWEAK ELISA Kit (人TWEAK ELISA试剂盒)

货号: KTE3139-2

产品类型: EliKine™ 双抗体夹心法ELISA定量试剂盒

反应性: 人

样本类型: 细胞培养上清、血清、血浆

产品概述

本试剂盒采用高灵敏度的双抗体夹心ELISA原理，专门用于定量检测人样本中的TWEAK（肿瘤坏死因子样凋亡微弱诱导剂）蛋白水平。适用于细胞功能研究、炎症机制探索及血管生成相关领域的科研应用。

主要特点与优势

• 高灵敏度：最低检测浓度达15.63 pg/mL，可精准检测低丰度样本
• 优异特异性：经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰
• 宽线性范围：15.63 pg/mL - 1,000 pg/mL，满足不同浓度样本检测需求
• 即用型组分：预包被微孔板、冻干标准品及优化缓冲液体系，操作简便

试剂盒组分

• Human TWEAK预包被微孔板
• Human TWEAK冻干标准品
• 标准品稀释液
• 10×检测缓冲液
• 100×生物素化检测抗体
• 100×链霉亲和素-HRP结合物
• TMB显色底物
• 终止液
• 20×洗涤缓冲液
• 板盖

技术参数

检测方法	双抗体夹心ELISA
检测范围	15.63 - 1,000 pg/mL
灵敏度	15.63 pg/mL
样本体积	建议100 μL/孔（具体参考说明书）
孵育时间	总时长约3-4小时（含样本孵育、洗板及显色步骤）

背景信息

TWEAK（肿瘤坏死因子样凋亡微弱诱导剂）是TNF超家族成员之一，主要由单核细胞、树突状细胞和NK细胞分泌。该因子通过调控细胞凋亡、炎症反应和血管生成过程，在组织修复、慢性炎症及纤维化疾病中发挥关键作用。

保存与运输

保存条件: 未开封试剂盒避光保存于2-8℃，保质期24个月；启用后各组分按说明书要求保存

运输条件: 冰袋（蓝冰）低温运输

注意事项

• 请勿混合不同批号试剂或使用过期组分
• 使用前所有试剂需室温平衡30分钟
• 推荐使用一次性吸头，避免交叉污染
• 未使用的板条需密封后4℃保存
• 建议样本和标准品进行复孔检测以提高准确性
• 显色过程需避光操作

规格与价格

规格	价格（元）	货期
48 T	1598.0	请咨询
96 T	2558.0	请咨询

重要声明: 本产品仅限科研使用，不适用于临床诊断或其他人类用途。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human TWEAK ELISA Kit (Human TWEAK ELISA Reagent Kit)

Part No.: KTE3139-2

Product Type: EliKine™ Double‑Antibody Sandwich ELISA Quantitative Kit

Reactivity: Human

Sample Types: Cell culture supernatants, serum, plasma

Product Overview

This kit utilizes a high‑sensitivity double‑antibody sandwich ELISA format for the quantitative determination of human TWEAK (TNF‑like weak inducer of apoptosis) protein levels. It is suitable for research applications in cell‑function studies, inflammation‑mechanism investigation, and angiogenesis‑related fields.

Key Features and Advantages

• High Sensitivity: Minimum detectable concentration of 15.63 pg/mL, enabling precise quantification of low‑abundance samples
• Excellent Specificity: Rigorously validated with no significant cross‑reactivity or background interference from related proteins
• Wide Linear Range: 15.63 pg/mL – 1,000 pg/mL, covering a broad spectrum of sample concentrations
• Ready‑to‑Use Components: Pre‑coated microplates, lyophilized standards, and optimized buffer system for convenient handling

Kit Components

• Human TWEAK Pre‑Coated Microplate
• Human TWEAK Lyophilized Standard
• Standard Diluent
• 10× Assay Buffer
• 100× Biotinylated Detection Antibody
• 100× Streptavidin‑HRP Conjugate
• TMB Chromogenic Substrate
• Stop Solution
• 20× Wash Buffer
• Plate Seal

Technical Specifications

Assay Method	Double‑Antibody Sandwich ELISA
Measurement Range	15.63 – 1,000 pg/mL
Sensitivity	15.63 pg/mL
Sample Volume	Recommended 100 μL per well (see manual for details)
Incubation Time	Approximately 3–4 hours total (including sample incubation, washing, and development)

Background Information

TWEAK (TNF‑like weak inducer of apoptosis) is a member of the TNF superfamily, predominantly secreted by monocytes, dendritic cells, and NK cells. It modulates apoptosis, inflammatory responses, and angiogenesis, playing pivotal roles in tissue repair, chronic inflammation, and fibrotic diseases.

Storage and Transportation

Storage Conditions: Store unopened kits protected from light at 2–8 °C for up to 24 months; after opening, each component should be stored as instructed in the manual.

Shipping Conditions: Ship on ice packs (blue ice) under refrigerated conditions.

Precautions

• Do not mix reagents from different lots or use expired components
• Equilibrate all reagents to room temperature for 30 min prior to use
• Use disposable pipette tips to avoid cross‑contamination
• Seal and store unused plates at 4 °C
• Perform duplicate wells for samples and standards to improve accuracy
• Conduct the color development step protected from light

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T	1598.0	Please inquire
96 T	2558.0	Please inquire

Important Statement: This product is for research use only and is not intended for clinical diagnosis or any other human application.

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakein Company for the most current details.