VEGF R1/Flt-1 ELISA KIT 血管内皮生长

Human VEGF R1/Flt-1 ELISA Kit (人血管内皮生长因子受体1 ELISA试剂盒)

货号：KTE3117-2

产品概述

本试剂盒是一款专门用于定量检测人血清、血浆及细胞培养上清样本中血管内皮生长因子受体1 (VEGF R1/Flt-1) 的高性能ELISA试剂盒。作为EliKine™系列核心产品，采用经典的夹心法ELISA原理，确保对目标蛋白具有极高的检测灵敏度和特异性，为血管生成、肿瘤研究及相关疾病机制探索提供可靠的分析工具。

产品特点与优势

• 高灵敏度：最低检测浓度达0.16 ng/mL，可精准检测低丰度样本。
• 优异特异性：经严格验证，与类似物无明显交叉反应或背景干扰。
• 宽检测范围：0.16 ng/mL - 10 ng/mL，适用于多种样本浓度的定量分析。
• 操作便捷：试剂盒组分齐全，包含标准品、检测抗体及显色底物，流程标准化。
• 重复性好：推荐复孔检测，确保实验结果的稳定与可靠。

检测原理

本试剂盒采用双抗体夹心ELISA法。预先包被在微孔板上的特异性抗体捕获样本中的VEGF R1，洗涤后加入生物素化检测抗体，再通过链霉亲和素-HRP偶联物与底物TMB进行显色反应。在450 nm波长下测定吸光度，VEGF R1浓度与OD值呈正相关，实现精准定量。

试剂盒组分

• Human VEGF R1 预包被微孔板
• Human VEGF R1 标准品（冻干）
• 标准品稀释液
• 检测抗体（100×）
• 链霉亲和素-HRP（100×）
• HRP底物（TMB）
• 终止液
• 洗涤缓冲液（20×）
• 板盖

样本类型

细胞培养上清、人血清、血浆（请注意避免反复冻融与细菌污染）

技术参数

检测范围	0.16 ng/mL - 10 ng/mL
灵敏度	0.16 ng/mL
反应性	人
检测方法	夹心法ELISA

产品规格与价格

规格	价格（元）	货期
48 T	1598.0	期货（约5个工作日内发货）
96 T	2558.0	期货（约5个工作日内发货）

保存与运输

未开封试剂盒避光保存于2-8℃，有效期24个月。开启后请参照说明书保存各组分。本品采用冰袋（蓝冰）运输，以确保试剂稳定性。

注意事项

• 请勿混用不同批号或过期试剂。
• 使用前请将试剂室温平衡30分钟。
• 实验过程中建议使用一次性吸头，避免交叉污染。
• 未使用的板条需立即密封，4℃保存。
• 为保证反应均匀，推荐使用低速振荡器或定期手动混匀。
• 为提高准确性，建议对样品与标准品进行复孔检测。

背景信息

血管内皮生长因子受体1 (VEGF R1/Flt-1) 是VEGF家族的关键受体之一，参与调控血管生成、细胞增殖等生理与病理过程。其可溶形式（sVEGF R1）在多种疾病状态（如肿瘤、心血管疾病、妊娠高血压）中具有潜在诊断价值。本试剂盒为研究VEGF R1在相关领域的表达与功能提供了专用工具。

警告：本产品仅用于科学研究，不适用于临床诊断或其他人类用途。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human VEGF R1/Flt-1 ELISA Kit

Catalog Number: KTE3117-2

Product Overview

This kit is a high-performance ELISA kit specifically designed for the quantitative detection of Human Vascular Endothelial Growth Factor Receptor 1 (VEGF R1/Flt-1) in human serum, plasma, and cell culture supernatant samples. As a core product of the EliKine™ series, it employs the classic sandwich ELISA principle, ensuring extremely high detection sensitivity and specificity for the target protein, providing a reliable analytical tool for angiogenesis, tumor research, and related disease mechanism exploration.

Features and Advantages

• High Sensitivity: Minimum detection concentration reaches 0.16 ng/mL, enabling precise detection of low-abundance samples.
• Excellent Specificity: Rigorously validated, with no significant cross-reactivity or background interference with similar substances.
• Broad Detection Range: 0.16 ng/mL - 10 ng/mL, suitable for quantitative analysis of various sample concentrations.
• Convenient Operation: The kit includes all necessary components, including standards, detection antibodies, and chromogenic substrates, with a standardized workflow.
• Good Reproducibility: Duplicate well testing is recommended to ensure stable and reliable experimental results.

Detection Principle

This kit uses a double-antibody sandwich ELISA method. The specific antibody pre-coated on the microplate captures VEGF R1 from the sample. After washing, a biotinylated detection antibody is added, followed by a streptavidin-HRP conjugate and the substrate TMB for color development. The absorbance is measured at 450 nm, and the VEGF R1 concentration is directly proportional to the OD value, enabling accurate quantification.

Kit Components

• Human VEGF R1 Pre-coated Microplate
• Human VEGF R1 Standard (Lyophilized)
• Standard Diluent
• Detection Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Sealer

Sample Types

Cell culture supernatant, human serum, plasma (Please avoid repeated freeze-thaw cycles and bacterial contamination)

Technical Parameters

Detection Range	0.16 ng/mL - 10 ng/mL
Sensitivity	0.16 ng/mL
Reactivity	Human
Detection Method	Sandwich ELISA

Specifications and Pricing

Specification	Price (USD)	Delivery Time
48 T	1598.0	Stock (Ships within approximately 5 business days)
96 T	2558.0	Stock (Ships within approximately 5 business days)

Storage and Shipping

The unopened kit should be stored protected from light at 2-8°C and is stable for 24 months. After opening, please refer to the instructions for storage of individual components. This product is shipped with ice packs (blue ice) to ensure reagent stability.

Precautions

• Do not mix reagents from different lot numbers or use expired reagents.
• Allow all reagents to reach room temperature (approx. 30 minutes) before use.
• Use disposable pipette tips throughout the assay to avoid cross-contamination.
• Immediately reseal any unused strips and store at 4°C.
• For uniform reactions, use a plate shaker at low speed or agitate manually at regular intervals.
• For improved accuracy, it is recommended to test samples and standards in duplicate.

Background Information

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1/Flt-1) is a key receptor in the VEGF family, involved in regulating physiological and pathological processes such as angiogenesis and cell proliferation. Its soluble form (sVEGF R1) has potential diagnostic value in various disease states (e.g., tumors, cardiovascular diseases, preeclampsia). This kit provides a specialized tool for studying the expression and function of VEGF R1 in related fields.

Warning: This product is for research use only. Not for use in diagnostic procedures or other human applications.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakonin Company for specific details.