MMP-13 ELISA KIT 基质金属蛋白酶13ELIS

Human MMP-13 ELISA Kit (人基质金属蛋白酶13 ELISA试剂盒)

货号: KTE3111-2 | 产品类型: EliKine™ ELISA定量试剂盒

产品概述

本试剂盒采用双抗体夹心法原理，专门用于定量检测人血清、血浆或细胞培养上清样本中的基质金属蛋白酶13 (MMP-13) 含量。MMP-13（胶原酶3）在骨骼发育、组织重塑及多种病理过程中（如关节炎、肿瘤转移）扮演关键角色。该试剂盒设计精密，确保了检测的高特异性和可靠性，适用于基础研究与药物开发领域的蛋白表达分析。

核心优势

• 高灵敏度：最低检测浓度达31.25 pg/mL，可精准捕获低丰度目标蛋白信号。
• 优异特异性：经严格验证，与类似蛋白无明显交叉反应，背景干扰低。
• 宽线性范围：检测范围为31.25 pg/mL - 2,000 pg/mL，满足多样本浓度需求。
• 操作便捷：预包被微孔板与即用型组分设计，简化实验流程，提升效率。

试剂盒组分

• 预包被Human MMP13微孔板
• Human MMP13标准品（冻干）
• 标准品稀释液
• 10×检测缓冲液
• 100×生物素化检测抗体
• 100×链霉亲和素-HRP偶联物
• TMB底物溶液
• 终止液
• 20×洗涤缓冲液
• 板盖

技术参数

检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆（人源）
检测范围	31.25 - 2,000 pg/mL
灵敏度	31.25 pg/mL
反应性	人
保存条件	未开封试剂盒避光保存于2-8℃，有效期24个月；启用后请按说明书保存各组分
运输条件	冰袋（蓝冰）运输

实验注意事项

• 请勿混用不同批号或过期试剂。
• 使用前所有组分需室温平衡30分钟。
• 推荐使用一次性吸头，避免交叉污染。
• 未使用的板条需立即密封，4℃保存。
• 实验过程中需充分混匀，建议使用低速振荡器或定期手动摇匀。
• 为保障结果准确性，样品与标准品建议进行复孔检测。

规格与价格

规格	价格（元）	货期
48 T	1598.0	请咨询
96 T	2558.0	请咨询

重要声明：本产品仅供科研使用，不适用于人类或临床诊断。使用前请确保符合相关专利或许可规定，厂家不对因使用本品导致的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human MMP-13 ELISA Kit

Catalog Number: KTE3111-2 | Product Type: EliKine™ ELISA Quantitative Kit

Product Overview

This kit employs a sandwich enzyme immunoassay technique for the quantitative detection of Matrix Metalloproteinase-13 (MMP-13) in human serum, plasma, or cell culture supernatants. MMP-13 (Collagenase 3) plays a critical role in bone development, tissue remodeling, and various pathological processes such as arthritis and tumor metastasis. The kit is meticulously designed to ensure high specificity and reliability, making it suitable for protein expression analysis in basic research and drug development.

Key Advantages

• High Sensitivity: Minimum detectable concentration is 31.25 pg/mL, enabling precise detection of low-abundance target proteins.
• Excellent Specificity: Rigorously validated to show no significant cross-reactivity with similar proteins, resulting in low background interference.
• Wide Dynamic Range: Detection range of 31.25 pg/mL - 2,000 pg/mL accommodates diverse sample concentrations.
• User-Friendly Protocol: Pre-coated microplate and ready-to-use components simplify the experimental procedure and enhance efficiency.

Kit Components

• Pre-coated Human MMP13 Microplate
• Human MMP13 Standard (Lyophilized)
• Standard Diluent
• 10× Assay Buffer
• 100× Biotinylated Detection Antibody
• 100× Streptavidin-HRP Conjugate
• TMB Substrate Solution
• Stop Solution
• 20× Wash Buffer
• Plate Sealer

Technical Parameters

Assay Type	Sandwich ELISA
Sample Type	Cell culture supernatant, serum, plasma (Human)
Detection Range	31.25 - 2,000 pg/mL
Sensitivity	31.25 pg/mL
Reactivity	Human
Storage Conditions	Store the unopened kit at 2-8°C protected from light; stable for 24 months. After first use, store components as directed in the manual.
Shipping Conditions	Shipped with ice packs (blue ice).

Experimental Notes

• Do not mix reagents from different lots or use expired reagents.
• Equilibrate all components to room temperature for 30 minutes before use.
• Use disposable pipette tips to avoid cross-contamination.
• Immediately reseal unused strips and store at 4°C.
• Ensure thorough mixing during the assay; use a low-speed shaker or periodic manual agitation is recommended.
• For accurate results, it is recommended to assay samples and standards in duplicate.

Specifications & Pricing

Size	Price (CNY)	Availability
48 T	1598.0	Please Inquire
96 T	2558.0	Please Inquire

Important Notice: This product is for research use only and is not intended for human or clinical diagnostic use. Ensure compliance with relevant patent or licensing regulations prior to use. The manufacturer is not liable for patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to product optimization and updates. For specific details, please contact Yakeyin Company.