Galectin-3 ELISA KIT 半乳糖凝集素-3E

Human Galectin‑3 ELISA Kit (Galectin‑3 ELISA KIT 半乳糖凝集素‑3 ELISA试剂盒)

货号：KTE3063‑2   |   检测类型：ELISA（夹心法）   |   物种：人

一、产品概述

本套装基于EliKine™ 双抗体夹心技术，可用于细胞培养上清、血清及血浆中 Galectin‑3 蛋白的定量分析。采用高灵敏度、特异性强的抗体组合，能够在 62.5 pg/mL–4,000 pg/mL 范围内实现线性检测。

二、核心技术与检测原理

1. 预包被抗 Galectin‑3 抗体的微孔板捕获样品中目标蛋白。
2. 添加生物素化的检测抗体形成二抗-抗原-捕获抗体三元复合物。
3. 加入链霉亲和素‑HRP 连接体，通过生物素‑链霉亲和素特异性相互作用实现信号放大。
4. 最后加入 TMB 底物，在 HRP 催化下产生蓝色显色，终止液将颜色转为黄色，在 450 nm 读取 OD 值，依据标准曲线计算样本浓度。

三、主要优势

• 检测灵敏度：62.5 pg/mL（最小可检出浓度）。
• 宽阔定量范围：62.5 pg/mL–4,000 pg/mL。
• 针对人源 Galectin‑3 的高特异性，已在交叉反应实验中未检测到显著背景信号。
• 适用于细胞培养上清、血清和血浆，可在单孔或复孔（双/三孔）设置中重复测定，提高结果可靠性。

四、试剂盒组成（每套）

• Human Galectin‑3 Microplate（96孔）
• Human Galectin‑3 Standard（冻干）
• Standard Diluent
• Assay Buffer (10×)
• Human Galectin‑3 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

五、使用注意事项

• 不同批号的试剂或超过有效期的试剂请勿混用。
• 使用前需在室温下平衡 30 分钟。
• 实验过程建议使用一次性吸头，防止交叉污染。
• 未用完的板条应立即放回原包装，密封后置于 4 ℃保存。
• 每 10 分钟轻摇或使用低速振荡器充分混匀，以确保反应均一。
• 建议对样品和标准品做双孔或三孔重复（复孔），以提升数据的统计可靠性。

六、储存与运输

储存：未开封试剂盒请避光保存于 2–8 ℃，保质期 24 个月；启用后请参照说明书中各组分的储存要求。

运输：采用蓝色冰袋冷链运输，确保全程温控。

七、背景信息

半乳糖凝集素‑3（Galectin‑3）属于凝集素家族，广泛分布于细胞核、胞质、线粒体、细胞表面及细胞外环境。它具备 β‑半乳糖苷酶亲和力，参与细胞黏附、活化、趋化、增殖、分化、细胞周期调控及凋亡等多项生物学过程。临床与基础研究表明，Galectin‑3 与肿瘤转移、纤维化（如肝、肾、特发性肺纤维化）、心血管疾病及中风等病理状态密切相关，因而成为重要的生物标志物。

八、规格与采购信息

规格	单价（人民币）	货期
48 T	1,598.00	请咨询
96 T	2,558.00	期货（5 天内发货）

九、法规声明

本产品仅供科研使用，不适用于人类或临床诊断。用户应自行确保使用过程不侵犯任何专利或许可证，因使用本产品可能产生的专利侵权或其他违规行为，本公司不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Galectin‑3 ELISA Kit (Galectin‑3 ELISA KIT)

Catalog Number: KTE3063‑2   |   Assay Type: ELISA (Sandwich)   |   Species: Human

1. Product Overview

This kit utilizes the EliKine™ double antibody sandwich technology for quantitative analysis of Galectin‑3 protein in cell culture supernatants, serum, and plasma. Employing a highly sensitive and specific antibody pair, it achieves linear detection within the range of 62.5 pg/mL–4,000 pg/mL.

2. Core Technology and Detection Principle

1. The microwell plate pre-coated with anti-Galectin‑3 antibody captures the target protein from the sample.
2. A biotinylated detection antibody is added to form a detection antibody-antigen-capture antibody ternary complex.
3. Streptavidin-HRP conjugate is added, achieving signal amplification via specific biotin-streptavidin interaction.
4. TMB substrate is finally added, producing a blue color catalyzed by HRP, which turns yellow upon addition of stop solution. The OD value is measured at 450 nm, and the sample concentration is calculated based on the standard curve.

3. Key Advantages

• Sensitivity: 62.5 pg/mL (minimum detectable concentration).
• Wide Quantification Range: 62.5 pg/mL–4,000 pg/mL.
• High specificity for human Galectin‑3, with no significant background signals detected in cross-reactivity studies.
• Suitable for cell culture supernatants, serum, and plasma. Repeatable measurements in single or replicate wells (duplicate/triplicate) enhance result reliability.

4. Kit Components (per kit)

• Human Galectin‑3 Microplate (96 wells)
• Human Galectin‑3 Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human Galectin‑3 Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

5. Precautions for Use

• Do not mix reagents from different lot numbers or expired reagents.
• Equilibrate all components to room temperature for 30 minutes before use.
• Use disposable pipette tips to prevent cross-contamination.
• Unused strips should be immediately returned to the original pouch, sealed, and stored at 4 ℃.
• Gently shake the plate or use a low-speed shaker every 10 minutes to ensure uniform reaction.
• It is recommended to perform duplicate or triplicate wells for samples and standards to improve statistical reliability.

6. Storage and Shipping

Storage: Unopened kits should be stored protected from light at 2–8 ℃; shelf life is 24 months. After opening, please refer to the storage requirements for individual components in the manual.

Shipping: Shipped with blue ice packs under controlled temperature conditions.

7. Background Information

Galectin‑3 belongs to the lectin family and is widely distributed in the nucleus, cytoplasm, mitochondria, cell surface, and extracellular environment. It possesses β-galactoside-binding affinity and is involved in various biological processes such as cell adhesion, activation, chemotaxis, proliferation, differentiation, cell cycle regulation, and apoptosis. Clinical and basic research indicate that Galectin‑3 is closely associated with pathological conditions including cancer metastasis, fibrosis (e.g., liver, kidney, idiopathic pulmonary fibrosis), cardiovascular diseases, and stroke, making it an important biomarker.

8. Specifications and Ordering Information

Specification	Unit Price (RMB)	Availability
48 T	1,598.00	Please inquire
96 T	2,558.00	Stock (Ships within 5 days)

9. Regulatory Statement

This product is for research use only, not intended for human or clinical diagnostic use. Users are responsible for ensuring that their use does not infringe any patents or licenses. The company assumes no liability for any patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please contact Yaken for specific details.