PDGF-BB ELISA KIT 血小板衍生生长因子BBE

Human/Rat PDGF‑BB ELISA Kit (KTE3060‑2)

中文名称：PDGF‑BB ELISA KIT 血小板衍生生长因子 BB ELISA试剂盒（人/大鼠）
英文名称：Human/Rat PDGF‑BB ELISA Kit
货号：KTE3060‑2

核心应用领域

• 产品分类：核心产品
• 产品中心：细胞分析
• 一级应用：ELISA检测
• 适用蛋白：PDGF‑BB（血小板衍生生长因子 BB）
• 检测类型：夹心（双抗体）ELISA
• 样本类型：细胞培养上清、血清、血浆
• 支持物种：人 / 大鼠

产品概述

EliKine™ Human/Rat PDGF‑BB ELISA Kit 采用双抗体夹心法，对样品中的 PDGF‑BB 进行定量分析。试剂盒中所有抗体均经过严格筛选，确保对人源和大鼠源 PDGF‑BB 均具备高特异性，显著降低同源蛋白及基质干扰。

关键技术特点 & 优势

• 高灵敏度：检测下限 31.25 pg/mL，覆盖 31.25 pg/mL ~ 2,000 pg/mL 的宽广定量范围。
• 特异性强：针对 PDGF‑BB 的专属性抗体未出现明显交叉反应。
• 稳定的信号输出：采用 TMB + HRP 体系，终止后产生黄色可在 450 nm 读取，信号与浓度呈线性关系。
• 操作简便：配套的标准稀释液、洗涤缓冲及板盖均为一步式使用，适合高通量筛选（48 或 96孔板）。
• 兼容多种样本基质：血清、血浆以及细胞培养上清均可直接检测，减少前处理步骤。

试剂盒完整组分

• Human/Rat PDGF‑BB Microplate（预包被）
• Human/Rat PDGF‑BB Standard（冻干）
• Standard Diluent
• Assay Buffer (10×)
• Human/Rat PDGF‑BB Detect Antibody (100×，生物素化)
• Streptavidin‑HRP (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers（防尘）

典型实验流程（概览）

1. 将试剂盒在使用前于室温平衡 30 分钟。
2. 加入 100 µL 样本或标准至预包被孔中，37 ℃孵育 1 h（或按需延长至 2 h）。
3. 洗板（使用 20× Wash Buffer），重复 3–5 次以去除未结合组分。
4. 加入 100 µL Detect Antibody，37 ℃孵育 30 min。
5. 洗板后加入 100 µL Streptavidin‑HRP，37 ℃孵育 20 min。
6. 洗板后加入 100 µL TMB 底物，避光反应 10–20 min至颜色发展。
7. 加入 50 µL Stop Solution，终止反应并在 450 nm 读取 OD。
8. 使用标准曲线（4‑parameter logistic）计算样本中 PDGF‑BB 浓度。

为保证数据可靠，推荐对每个样本进行双孔或三孔复孔检测。

性能参数

检测范围	31.25 pg/mL ~ 2,000 pg/mL
灵敏度（LOD）	31.25 pg/mL
样本体积	≤ 100 µL（根据孔板规格）
测定时间	约 2.5 h（含洗涤）

存储 & 运输

• 未开封试剂盒：避光保存于 2‑8 ℃，有效期 24 个月。
• 开封后：请参照说明书分别存放各组分（如抗体、酶标底物等），并在使用完毕后将板条密闭置于 4 ℃。
• 运输方式：蓝冰（冰袋）冷链运输，确保全程温度控制。

使用注意事项

• 不同批号或已过期的试剂不得混合使用。
• 实验中请使用一次性吸头，防止交叉污染。
• 未使用的板条需及时放回包装并密封保存。
• 充分混匀是获得可靠信号的关键，建议使用低速振荡器或每 10 min 手动轻摇。
• 样本及标准建议采用双孔或三孔复检，以降低偶然误差。

合规声明

本产品仅供科研使用，不适用于临床诊断或治疗。使用时请遵守相关专利与许可证规定，本公司对因不当使用导致的任何专利侵权或其他违规行为不承担责任。

订购信息

规格	单位	单价 (CNY)	交货期
48 T	套	1,598.00	请咨询
96 T	套	2,558.00	请咨询

产品背景（科研价值）

PDGF‑BB 是调控细胞增殖、血管生成及组织修复的关键因子。它以二聚体（AA、BB或AB）形式存在，在肿瘤血管生成、骨重建及伤口愈合等生理病理过程中发挥重要作用。通过本套件精准测定人源或大鼠源 PDGF‑BB，可帮助您在以下研究方向获得定量数据：

• 血管生成与抗血管生成药物的作用机理研究。
• 骨代谢、成骨细胞活性以及骨相关疾病模型的评价。
• 炎症或创伤模型中巨噬细胞、成纤维细胞的活化状态监测。
• 肿瘤微环境中 PDGF‑BB 的动态变化及其与其他生长因子的相互作用。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human/Rat PDGF‑BB ELISA Kit (KTE3060‑2)

Chinese Name: PDGF‑BB ELISA KIT 血小板衍生生长因子 BB ELISA试剂盒（人/大鼠）
English Name: Human/Rat PDGF‑BB ELISA Kit
Catalog Number: KTE3060‑2

Core Application Areas

• Product Category: Core Product
• Product Center: Cell Analysis
• Primary Application: ELISA Detection
• Target Protein: PDGF‑BB (Platelet-Derived Growth Factor BB)
• Assay Type: Sandwich (Two-Antibody) ELISA
• Sample Types: Cell Culture Supernatant, Serum, Plasma
• Supported Species: Human / Rat

Product Overview

The EliKine™ Human/Rat PDGF‑BB ELISA Kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) principle for the quantitative measurement of PDGF‑BB in samples. All antibodies included in the kit have been rigorously screened to ensure high specificity for both human and rat PDGF‑BB, significantly reducing interference from homologous proteins and sample matrices.

Key Technical Features & Advantages

• High Sensitivity: Low detection limit of 31.25 pg/mL, with a broad quantitative range from 31.25 pg/mL to 2,000 pg/mL.
• Strong Specificity: Specific antibodies against PDGF‑BB show no significant cross-reactivity.
• Stable Signal Output: Utilizes TMB + HRP system; after stopping, the developed yellow color can be read at 450 nm, with signal intensity linearly related to concentration.
• User-Friendly Operation: Includes ready-to-use standard diluent, wash buffer, and plate covers, suitable for high-throughput screening (48 or 96-well plates).
• Compatibility with Various Sample Matrices: Serum, plasma, and cell culture supernatant can be directly assayed, minimizing pre-treatment steps.

Complete Kit Components

• Human/Rat PDGF‑BB Microplate (Pre-coated)
• Human/Rat PDGF‑BB Standard (Lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Human/Rat PDGF‑BB Detect Antibody (100×, Biotinylated)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers (Dust-proof)

Typical Experimental Procedure (Overview)

1. Equilibrate the kit at room temperature for 30 minutes before use.
2. Add 100 µL of sample or standard to the pre-coated wells. Incubate at 37 °C for 1 h (or extend to 2 h if needed).
3. Wash the plate (using 20× Wash Buffer) 3–5 times to remove unbound components.
4. Add 100 µL of Detect Antibody. Incubate at 37 °C for 30 min.
5. After washing, add 100 µL of Streptavidin‑HRP. Incubate at 37 °C for 20 min.
6. After washing, add 100 µL of TMB substrate. Incubate protected from light for 10–20 min for color development.
7. Add 50 µL of Stop Solution to terminate the reaction. Read the Optical Density (OD) at 450 nm.
8. Calculate the concentration of PDGF‑BB in samples using the standard curve (4-parameter logistic fit).

For reliable data, it is recommended to perform duplicate or triplicate measurements for each sample.

Performance Parameters

Detection Range	31.25 pg/mL to 2,000 pg/mL
Sensitivity (LOD)	31.25 pg/mL
Sample Volume	≤ 100 µL (depending on plate format)
Assay Time	Approximately 2.5 h (including washing steps)

Storage & Shipping

• Unopened Kit: Store protected from light at 2‑8 °C. Valid for 24 months.
• After Opening: Store individual components (e.g., antibodies, enzyme substrate) as per instructions. After use, reseal the unused strips and store at 4 °C.
• Shipping Method: Shipped with blue ice packs under controlled temperature conditions.

Usage Precautions

• Do not mix reagents from different lot numbers or expired kits.
• Use disposable pipette tips to prevent cross-contamination.
• Return unused strips to their pouch and seal promptly.
• Thorough mixing is crucial for reliable signals; use a low-speed shaker or gentle manual shaking every 10 min.
• Perform duplicate or triplicate measurements for samples and standards to minimize random errors.

Compliance Statement

This product is intended for research use only. Not for use in clinical diagnosis or treatment. Please comply with relevant patent and licensing regulations. The company assumes no responsibility for any patent infringement or other violations resulting from improper use.

Ordering Information

Size	Unit	Unit Price (CNY)	Delivery Time
48 T	Set	1,598.00	Please Inquire
96 T	Set	2,558.00	Please Inquire

Product Background (Scientific Value)

PDGF‑BB is a key factor regulating cell proliferation, angiogenesis, and tissue repair. It exists as a dimer (AA, BB, or AB) and plays significant roles in physiological and pathological processes such as tumor angiogenesis, bone remodeling, and wound healing. Precise quantification of human or rat PDGF‑BB using this kit provides quantitative data for research areas including:

• Mechanism studies of angiogenesis and anti-angiogenic drugs.
• Evaluation of bone metabolism, osteoblast activity, and bone-related disease models.
• Monitoring activation status of macrophages and fibroblasts in inflammation or wound models.
• Investigating dynamic changes of PDGF‑BB in the tumor microenvironment and its interactions with other growth factors.

Note: The above information is subject to change due to product optimization and updates. For specific details, please consult Yakeyin Company.