Cortisol ELISA KIT 皮质醇竞争法ELISA

产品概述

EliKine™ Cortisol竞争法ELISA试剂盒（货号：KTE3059-2）是一款专为精准定量人、小鼠、大鼠样本中皮质醇含量而设计的高性能检测工具。本试剂盒属于核心产品线，严格遵循ELISA标准流程，适用于细胞培养上清、血清及血浆等多种样本类型。

核心特性与优势

• 高灵敏度与特异性：最低检测浓度达0.117 ng/mL，检测范围为0.117-15 ng/mL。经严格验证，对目标蛋白类似物无明显交叉反应或背景干扰。
• 广谱反应性：支持人、小鼠、大鼠三种物种的皮质醇检测，满足多物种研究需求。
• 稳定可靠的组分：试剂盒包含预包被微孔板、特异性抗体、标准品、缓冲液等完整组分，确保实验流程的连贯性与结果的一致性。
• 竞争法检测原理：通过HRP标记皮质醇与样本中皮质醇竞争结合有限抗体位点，实现精准定量，颜色强度与皮质醇浓度成反比。

技术参数

产品名称	Cortisol竞争法ELISA试剂盒（人）
检测方法	竞争性ELISA
样本类型	细胞培养上清、血清、血浆
检测范围	0.117 ng/mL - 15 ng/mL
灵敏度	0.117 ng/mL
保存条件	未开封试剂盒避光保存于2-8℃，保质期24个月

试剂盒组分

• Cortisol Microplate
• Cortisol Antibody (100×)
• Cortisol Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Dissociation Reagent
• HRP Conjugated Cortisol (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

应用背景

皮质醇（Cortisol）是一种由肾上腺皮质分泌的糖皮质激素类固醇激素，在应激反应、血糖调节和免疫抑制中起关键作用。其分泌呈现昼夜节律性，晨间达到峰值。异常皮质醇水平与库欣综合征、抑郁症、前列腺癌等疾病密切相关。本试剂盒通过竞争性ELISA技术，为研究皮质醇在代谢、免疫及疾病机制中的功能提供可靠工具。

规格与订购信息

规格	价格（元）	货期
48 T	1598.0	期货（5天内发货）
96 T	2558.0	期货（5天内发货）

使用注意事项

• 请勿混合不同批号或过期试剂使用。
• 使用前需室温平衡30分钟，避免交叉污染。
• 推荐使用复孔检测（双孔或三孔）以提高结果可靠性。
• 未使用的板条需立即密封并于4℃保存。
• 实验过程中需充分混匀样本与试剂。

运输条件：冰袋运输（蓝冰）

警告：本产品仅供研究使用，不适用于人类或临床诊断。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

EliKine™ Cortisol Competitive ELISA Kit (Catalog No.: KTE3059-2) is a high‑performance assay designed for the precise quantitation of cortisol in human, mouse, and rat samples. This kit is part of the core product line, follows standard ELISA protocols, and is compatible with cell culture supernatants, serum, and plasma.

Key Features and Advantages

• High Sensitivity and Specificity: Minimum detection limit of 0.117 ng/mL, with a quantitative range of 0.117–15 ng/mL. Validation confirms negligible cross‑reactivity or background interference with related proteins.
• Broad Species Reactivity: Supports cortisol measurement in human, mouse, and rat samples, addressing multi‑species research needs.
• Stable and Reliable Components: The kit includes pre‑coated microplates, specific antibodies, standards, buffers, and other reagents, ensuring workflow continuity and result consistency.
• Competitive Assay Principle: HRP‑conjugated cortisol competes with sample cortisol for a limited antibody binding site, enabling precise quantitation; color development is inversely proportional to cortisol concentration.

Technical Specifications

Product Name	Cortisol Competitive ELISA Kit (Human)
Assay Method	Competitive ELISA
Sample Types	Cell culture supernatant, serum, plasma
Detection Range	0.117 ng/mL – 15 ng/mL
Sensitivity	0.117 ng/mL
Storage Conditions	Unopened kits should be protected from light and stored at 2–8 °C; shelf life 24 months.

Kit Components

• Cortisol Microplate
• Cortisol Antibody (100×)
• Cortisol Standard (lyophilized)
• Standard Diluent
• Assay Buffer (10×)
• Dissociation Reagent
• HRP Conjugated Cortisol (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Application Background

Cortisol is a glucocorticoid steroid hormone secreted by the adrenal cortex, playing essential roles in stress response, glucose homeostasis, and immunosuppression. Its secretion follows a circadian rhythm, peaking in the early morning. Aberrant cortisol levels are closely associated with Cushing’s syndrome, depression, prostate cancer, and other disorders. This kit utilizes competitive ELISA technology to provide a reliable tool for investigating cortisol’s functions in metabolism, immunity, and disease mechanisms.

Specifications and Ordering Information

Specification	Price (CNY)	Lead Time
48‑test	1,598.0	In stock (ship within 5 days)
96‑test	2,558.0	In stock (ship within 5 days)

Precautions

• Do not mix reagents from different lot numbers or use expired reagents.
• Allow all components to equilibrate to room temperature for 30 minutes before use; avoid cross‑contamination.
• It is recommended to perform replicate wells (duplicate or triplicate) to improve data reliability.
• Seal any unused plate strips immediately and store at 4 °C.
• Ensure thorough mixing of samples and reagents during the assay.

Shipping Conditions: Ice‑pack shipment (blue ice)

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications.

Note: The above information may change with product improvements or upgrades; please contact EliKine™ for the latest details.