Osteoprotegerin/OPG ELISA KIT

Human Osteoprotegerin/OPG ELISA Kit (人骨保护素ELISA试剂盒)

货号: KTE3055-2 | 产品分类: 核心产品 | 产品中心: 细胞分析

产品概述

本试剂盒是一款基于双抗体夹心法原理的高灵敏度定量检测试剂盒，专门用于精确检测人血清、血浆或细胞培养上清等样本中的骨保护素（Osteoprotegerin, OPG）浓度。作为EliKine™品牌下的核心产品，该试剂盒在设计上充分考虑了科研实验对数据准确性和重复性的高要求，适用于骨骼代谢、心血管疾病关联性等前沿领域的深入研究。

核心特性与优势

• 高灵敏度与特异性：最低检测限低至62.5 pg/mL，确保对低丰度样本的精准检测。经严格验证，与目标蛋白的类似物无明显交叉反应，有效降低背景干扰。
• 宽广的检测范围：检测范围为62.5 pg/mL - 4,000 pg/mL，能够覆盖绝大多数生理及病理状态下样本中的OPG浓度。
• 优异的批间一致性：精密的生产工艺确保了不同批次产品间的高度稳定性，为长期研究项目提供可靠的数据支持。
• 操作流程优化：预包被的微孔板及即用型或简易稀释的试剂组分，简化了实验步骤，有助于提升实验效率。

检测原理简介

采用经典的双抗体夹心ELISA法。首先，样本中的OPG与包被于微孔板上的特异性抗体结合。洗涤后，加入生物素标记的检测抗体与之形成夹心复合物。随后加入链霉亲和素-HRP偶联物，并通过TMB底物显色。颜色的深浅与样本中的OPG浓度成正比，可在450 nm波长下进行定量测定。

试剂盒组分

• 预包被Human OPG微孔板
• Human OPG标准品（冻干）
• 标准品稀释液
• 检测缓冲液（10×）
• Human OPG检测抗体（100×）
• 链霉亲和素-HRP（100×）
• HRP底物（TMB）
• 终止液
• 洗涤缓冲液（20×）
• 板盖

注：详细组分含量及复溶/稀释方法请参阅产品说明书。

样本类型

适用于人源样本，包括细胞培养上清、血清、血浆。

保存与运输

保存条件：未启封试剂盒请避光保存于2-8℃，有效期24个月。启封后各组分请按说明书建议条件保存。

运输条件：全程采用冰袋（蓝冰）低温运输，以确保试剂活性。

重要注意事项

• 请勿混合使用不同批号的试剂或已过有效期的试剂。
• 使用前，所有试剂应在室温平衡约30分钟。
• 为避免交叉污染，实验过程中请使用一次性吸头。
• 未使用的板条需立即放回原包装袋并密封，于4℃保存。
• 充分的混匀对实验结果至关重要，建议使用低速振荡器或定期手动摇匀。
• 为获得更可靠的数据，建议对标准品和样品进行复孔（双孔或三孔）检测。

产品规格与价格

规格	价格 (元)	货期
48 T	1598.0	期货（预计5个工作日内发货）
96 T	2558.0	期货（预计5个工作日内发货）

背景信息

骨保护素（OPG），也称为破骨细胞抑制因子或TNFRSF11B，是肿瘤坏死因子受体超家族成员。它通过抑制破骨细胞前体的分化来调节骨吸收，在维持骨密度方面发挥关键作用。近年研究表明，OPG水平与心血管疾病、严重精神障碍及糖尿病患者的血管风险存在潜在关联，使其成为连接骨骼与心血管系统研究的重要分子靶点。

警告： 本产品仅限于科研用途，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定，生产商对此类行为不承担任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Osteoprotegerin/OPG ELISA Kit (Human Osteoprotegerin/OPG ELISA Kit)

Catalog Number: KTE3055-2 | Product Classification: Core Products | Product Center: Cell Analysis

Product Overview

This kit is a highly sensitive quantitative detection kit based on the double antibody sandwich principle, specifically designed for accurately measuring the concentration of Osteoprotegerin (OPG) in samples such as human serum, plasma, or cell culture supernatants. As a core product under the EliKine™ brand, the kit is designed with a focus on the high demands of scientific research for data accuracy and reproducibility, making it suitable for in-depth studies in frontier areas such as bone metabolism and the association with cardiovascular diseases.

Core Features and Advantages

• High Sensitivity and Specificity: The minimum detection limit is as low as 62.5 pg/mL, ensuring accurate detection of low-abundance samples. Rigorous validation shows no cross-reactivity with analogs of the target protein, effectively reducing background interference.
• Broad Detection Range: The detection range is from 62.5 pg/mL to 4,000 pg/mL, covering the OPG concentration in the vast majority of samples under physiological and pathological conditions.
• Excellent Inter-assay Consistency: Precision manufacturing processes ensure high stability between different batches of the product, providing reliable data support for long-term research projects.
• Optimized Operational Workflow: Pre-coated microplates and ready-to-use or easily diluted reagent components simplify experimental steps, helping to improve experimental efficiency.

Brief Detection Principle

Utilizes the classic double antibody sandwich ELISA method. First, OPG in the sample binds to the specific antibody coated on the microplate. After washing, a biotin-labeled detection antibody is added to form a sandwich complex. Streptavidin-HRP conjugate is then added, followed by color development with the TMB substrate. The intensity of the color is proportional to the OPG concentration in the sample and can be quantitatively measured at a wavelength of 450 nm.

Kit Components

• Pre-coated Human OPG Microplate
• Human OPG Standard (lyophilized)
• Standard Diluent
• Detection Buffer (10×)
• Human OPG Detection Antibody (100×)
• Streptavidin-HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Sealers

Note: Please refer to the product instructions for detailed component contents and reconstitution/dilution methods.

Sample Types

Suitable for human-derived samples, including cell culture supernatants, serum, and plasma.

Storage and Shipping

Storage Conditions: Unopened kits should be stored protected from light at 2-8°C and are valid for 24 months. After opening, components should be stored according to the conditions recommended in the instructions.

Shipping Conditions: Shipment is conducted with ice packs (blue ice) to maintain low temperatures throughout, ensuring reagent activity.

Important Precautions

• Do not mix reagents from different lot numbers or reagents that have expired.
• Before use, all reagents should be equilibrated to room temperature for approximately 30 minutes.
• To avoid cross-contamination, use disposable pipette tips during the experiment.
• Unused strips should be immediately returned to their original packaging bag, sealed, and stored at 4°C.
• Adequate mixing is crucial for experimental results; it is recommended to use a low-speed shaker or perform regular manual shaking.
• For more reliable data, it is advised to perform duplicate (double or triple) measurements for both standards and samples.

Product Specifications and Pricing

Specification	Price (CNY)	Availability
48 T	1598.0	Futures (Estimated shipment within 5 business days)
96 T	2558.0	Futures (Estimated shipment within 5 business days)

Background Information

Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor or TNFRSF11B, is a member of the tumor necrosis factor receptor superfamily. It regulates bone resorption by inhibiting the differentiation of osteoclast precursors and plays a key role in maintaining bone density. Recent studies suggest that OPG levels may be potentially linked to cardiovascular disease risk, severe mental disorders, and vascular risk in diabetic patients, making it an important molecular target for research connecting the skeletal and cardiovascular systems.

Warning: This product is intended for research use only and is not suitable for human or clinical diagnosis. Users should ensure that their application does not violate any patent or license regulations; the manufacturer assumes no responsibility for such actions.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific information, please consult Yakeen Company.