T4 ELISA KIT 甲状腺素ELISA试剂盒(兔子)

产品概述

英文名称：Thyroxine (T4) ELISA Kit
中文名称：T4 ELISA KIT 甲状腺素ELISA试剂盒（兔子）
货号：KTE0007-B6-2
产品分类：核心产品 → 细胞分析 → ELISA
适用领域：蛋白类检测，尤其是ELISA平台的甲状腺功能研究。

关键特性 & 优势

• 高灵敏度：检测下限 10 ng/ml，覆盖 20–320 ng/ml 的宽动态范围。
• 高特异性：采用竞争法检测，未发现与其他甲状腺激素或血清蛋白的显著交叉反应。
• 多物种兼容性：适用于人、小鼠、大鼠、山羊、绵羊、狗、兔子、牛、猴子、鸡、猪等多达11种样本来源。
• 标准化批次：每批次均含预包被微孔板、标准品、标记T4等完整组分，确保实验重复性。

试剂盒组成

本套件提供完整的竞争ELISA检测所需试剂：

• T4 抗体预包被微孔板
• T4 标准品（梯度浓度）
• 生物素偶联的T4（竞争底物）
• Avidin‑HRP 复合物
• HRP 底物 A 与 B（显色剂）
• 反应终止液
• 洗涤缓冲液
• 封板膜（防止蒸发）

检测原理

本套件基于竞争法 ELISA：

1. 微孔板已预包被针对甲状腺素（T4）的特异性抗体。
2. 样本中的 T4 与生物素标记的 T4 竞争结合抗体。
3. 加入 Avidin‑HRP 复合物后，结合的生物素标记 T4 被检测。
4. 通过 HRP 底物显色，光密度 (OD) 与样本中 T4 含量呈负相关。
5. 使用标准曲线进行定量计算，得到血清或其他生物液体中 T4 浓度。

检测范围 & 灵敏度

检测范围	20 ng/ml – 320 ng/ml
灵敏度（LOQ）	10 ng/ml

适用样本类型

本试剂盒兼容以下样本基质：

• 血清
• 血浆
• 其他生物液体（根据实验需求可自行验证）

使用注意事项

• 不同批号或已超期的试剂不得混用。
• 使用前请将所有试剂在室温 (20‑25 °C) 平衡 30 分钟。
• 实验过程中务必使用一次性吸头，以防交叉污染。
• 未使用的微孔板应立即放回原包装，密闭保存于 4 °C。
• 充分混匀是获得可靠结果的关键，推荐使用低速振荡器或每 10 分钟手动轻摇一次。
• 样品和标准品建议设置双孔或三孔平行（复孔）以提高数据可靠性。

保存与运输

• 未开封存储：2‑8 °C（冰箱）
• 启用后：请参考随盒说明书中各组分的具体保存要求。
• 运输条件：全程使用蓝色冰袋冷链运输，以确保活性。

规格 & 价格

规格	包装	单价（元）	货期
48 试剂	48 T	1 598.0	期货（4 天内发货）
96 试剂	96 T	2 558.0	期货（4 天内发货）

法规声明 & 免责声明

本产品仅供科研用途，**不适用于人类临床诊断**。使用本套件进行的实验应遵循所在机构的伦理与合规要求。我们不对因使用本产品而产生的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Thyroxine (T4) ELISA Kit
Chinese Name: T4 ELISA KIT Thyroxine ELISA Kit (Rabbit)
Catalog Number: KTE0007-B6-2
Product Classification: Core Products → Cell Analysis → ELISA
Application: Protein detection, particularly for thyroid function research using the ELISA platform.

Key Features & Advantages

• High Sensitivity: Lower limit of detection 10 ng/ml, covering a wide dynamic range of 20–320 ng/ml.
• High Specificity: Utilizes a competitive assay format; no significant cross-reactivity observed with other thyroid hormones or serum proteins.
• Multi-Species Compatibility: Suitable for samples from up to 11 species, including human, mouse, rat, goat, sheep, dog, rabbit, bovine, monkey, chicken, and pig.
• Standardized Lots: Each lot includes complete components such as pre-coated microplate, standards, labeled T4, ensuring experimental reproducibility.

Kit Components

This kit provides all reagents required for a complete competitive ELISA assay:

• T4 Antibody Pre-coated Microplate
• T4 Standards (gradient concentrations)
• Biotin-Conjugated T4 (Competitive Substrate)
• Avidin-HRP Conjugate
• HRP Substrate A & B (Chromogen)
• Stop Solution
• Wash Buffer
• Plate Sealer (to prevent evaporation)

Assay Principle

This kit is based on a Competitive ELISA:

1. The microplate is pre-coated with a specific antibody against Thyroxine (T4).
2. T4 in the sample competes with biotin-labeled T4 for binding to the antibody.
3. After adding the Avidin-HRP conjugate, the bound biotin-labeled T4 is detected.
4. Color development via HRP substrate occurs, where the optical density (OD) is inversely proportional to the T4 concentration in the sample.
5. Quantification is performed using a standard curve to determine the T4 concentration in serum or other biological fluids.

Detection Range & Sensitivity

Detection Range	20 ng/ml – 320 ng/ml
Sensitivity (LOQ)	10 ng/ml

Suitable Sample Types

This kit is compatible with the following sample matrices:

• Serum
• Plasma
• Other biological fluids (validation may be required based on experimental needs)

Usage Precautions

• Do not mix reagents from different lots or expired kits.
• Allow all reagents to equilibrate to room temperature (20‑25 °C) for 30 minutes before use.
• Use disposable pipette tips throughout the procedure to prevent cross-contamination.
• Unused microplate strips should be immediately returned to the original packaging and stored sealed at 4 °C.
• Thorough mixing is crucial for reliable results; use a low-speed shaker or manually shake gently every 10 minutes.
• It is recommended to run samples and standards in duplicate or triplicate to enhance data reliability.

Storage & Shipping

• Unopened Storage: 2‑8 °C (refrigerator)
• After Opening: Please refer to the specific storage requirements for each component in the accompanying instruction manual.
• Shipping Conditions: Shipped with blue ice packs throughout the cold chain to ensure activity.

Specifications & Pricing

Specification	Packaging	Unit Price (CNY)	Lead Time
48 Tests	48 T	1 598.0	Stock (Ships within 4 days)
96 Tests	96 T	2 558.0	Stock (Ships within 4 days)

Regulatory Statement & Disclaimer

This product is intended for research use only, **not for human clinical diagnosis**. Experiments conducted using this kit should comply with the ethical and regulatory requirements of the user's institution. We assume no liability for patent infringement or other violations arising from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.