fT3 ELISA KIT 游离三碘甲状腺原氨酸ELISA试

产品概述

英文名称：Free Triiodothyronine (fT3) ELISA Kit
中文名称：fT3 ELISA KIT 游离三碘甲状腺原氨酸ELISA试剂盒（犬）
货号：KTE0004-B5-2

本套装属细胞分析中心核心产品，专为ELISA平台设计，采用竞争法定量检测血清、血浆及其他生物液体中的游离三碘甲状腺原氨酸（fT3）。适用于犬等多物种的甲状腺功能研究。

关键特性与优势

• 高灵敏度：检测下限 2 pmol/L。
• 宽检测范围：2 pmol/L – 32 pmol/L。
• 特异性强：在已评估的交叉反应中未发现显著干扰。
• 多物种适用：人、小鼠、大鼠、山羊、绵羊、狗、兔子、牛、猴子、鸡、猪。
• 即用型试剂盒：包含预包被微孔板及全套缓冲液，免除二次稀释步骤。

试剂盒组分

• fT3 抗体预包被微孔板
• fT3 标准品（系列浓度）
• 生物素偶联的 fT3（竞争分子）
• Avidin‑HRP
• HRP 底物 A、B（双底物显色体系）
• 反应终止液
• 洗涤缓冲液
• 封板膜

检测原理（竞争法 ELISA）

样本中的游离 fT3 与加入的生物素‑fT3 竞争结合预包被在微孔底部的抗体。结合后加入 Avidin‑HRP，随后加入双底物 A/B 产生颜色反应。颜色强度与样本中 fT3 含量呈负相关，使用随盒提供的标准曲线进行定量计算。

适用样本类型

• 血清
• 血浆
• 其他生物液体（如组织匀浆）

实验操作要点

• 所有试剂仅限本批次使用，避免与其他批号或过期试剂混用。
• 使用前请将试剂在室温（20‑25 ℃）平衡 30 分钟。
• 推荐使用一次性吸头，防止交叉污染。
• 未使用的板条请立即放回原包装，密闭保存于 4 ℃。
• 混匀是关键步骤，建议采用低速振荡器或每 10 分钟手动摇匀一次。
• 样品和标准品建议设置双孔或三孔复孔，以提高结果的可靠性。

存储与运输

• 未开封试剂盒：2 ℃ – 8 ℃冷藏保存。
• 开启后请参照说明书分别保存各组分。
• 运输方式：蓝色冰袋（低温）运输，确保全程冷链。

技术规格

规格	单价（元）	交货周期
48 T	1 598.0	期货（4 天内发货）
96 T	2 558.0	期货（4 天内发货）

应用背景

甲状腺功能的评估常依赖血清中总甲状腺素（T4）和总三碘甲状腺原氨酸（T3）的测定。>99 % 的 T4、T3 与甲状腺素结合球蛋白（TBG）结合，仅 游离 形态（fT4、fT3）具备生物活性，直接反映甲状腺的功能状态。fT3 ELISA 试剂盒可在动物模型或临床前研究中，快速获取该关键指标，为药效评价、疾病模型构建及代谢研究提供可靠数据。

注意事项与声明

• 本产品仅供科研使用，不得用于人类临床诊断。
• 请遵循所在地区的专利及许可证要求，使用本产品不构成对任何第三方权利的侵害。
• 因使用本产品导致的任何专利侵权或其他违规行为，供应商不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

English Name: Free Triiodothyronine (fT3) ELISA Kit
Chinese Name: fT3 ELISA KIT Free Triiodothyronine ELISA Kit (Canine)
Catalog Number: KTE0004-B5-2

This kit is a core product of the Cell Analysis Center, specifically designed for the ELISA platform. It employs a competitive method to quantitatively detect Free Triiodothyronine (fT3) in serum, plasma, and other biological fluids. It is suitable for thyroid function research in multiple species, including canine.

Key Features and Advantages

• High Sensitivity: Lower limit of detection 2 pmol/L.
• Wide Detection Range: 2 pmol/L – 32 pmol/L.
• High Specificity: No significant interference observed in evaluated cross-reactivities.
• Multi-Species Applicability: Human, mouse, rat, goat, sheep, dog, rabbit, bovine, monkey, chicken, porcine.
• Ready-to-Use Kit: Includes pre-coated microplate and a full set of buffers, eliminating the need for secondary dilution steps.

Kit Components

• fT3 Antibody Pre-coated Microplate
• fT3 Standards (serial concentrations)
• Biotin-Conjugated fT3 (Competitor)
• Avidin-HRP
• HRP Substrate A, B (Dual-substrate chromogenic system)
• Stop Solution
• Wash Buffer
• Plate Sealer

Detection Principle (Competitive ELISA)

Free fT3 in the sample competes with the added biotin-fT3 for binding to the antibody pre-coated on the bottom of the microplate wells. After binding, Avidin-HRP is added, followed by the dual substrates A/B to produce a color reaction. The color intensity is inversely proportional to the amount of fT3 in the sample, and quantification is calculated using the standard curve provided with the kit.

Applicable Sample Types

• Serum
• Plasma
• Other Biological Fluids (e.g., tissue homogenates)

Key Experimental Procedures

• All reagents are for use only in this specific batch; avoid mixing with reagents from other batches or expired reagents.
• Equilibrate all reagents to room temperature (20-25 °C) for 30 minutes before use.
• Use disposable pipette tips to prevent cross-contamination.
• Immediately return unused strips to the original packaging and store sealed at 4 °C.
• Thorough mixing is a critical step; use a low-speed shaker or manually shake every 10 minutes.
• It is recommended to set up duplicate or triplicate wells for both samples and standards to enhance result reliability.

Storage and Shipping

• Unopened Kit: Store at 2 °C – 8 °C.
• After Opening: Refer to the instructions for separate storage of each component.
• Shipping Method: Shipped with blue ice packs (low temperature) to ensure a cold chain throughout transit.

Technical Specifications

Specification	Unit Price (CNY)	Delivery Lead Time
48 T	1,598.0	Stock (Ships within 4 days)
96 T	2,558.0	Stock (Ships within 4 days)

Application Background

Evaluation of thyroid function often relies on the measurement of total thyroxine (T4) and total triiodothyronine (T3) in serum. >99% of T4 and T3 are bound to thyroxine-binding globulin (TBG); only the free forms (fT4, fT3) are biologically active and directly reflect the functional status of the thyroid gland. The fT3 ELISA Kit enables rapid acquisition of this key parameter in animal models or preclinical studies, providing reliable data for efficacy evaluation, disease model construction, and metabolic research.

Precautions and Disclaimer

• This product is for research use only and not for use in human clinical diagnosis.
• Please comply with patent and licensing requirements in your region. Use of this product does not constitute infringement of any third-party rights.
• The supplier is not liable for any patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.