人白介素-1α ELISA定量试剂盒(IL-1α)

产品概述

EliKine™ 人白介素-1α (IL-1α) ELISA定量试剂盒是一款专门设计用于精准定量人源样本中IL-1α蛋白含量的核心工具。本试剂盒采用经典的夹心法ELISA原理，具备优异的性能参数，旨在为生命科学研究领域的细胞因子分析提供可靠、重复性高的数据支持。

核心特性与优势

• 高灵敏度与特异性：最低检测浓度可达4 pg/mL，确保对低丰度样本的精准捕获。经过严格验证，与目标蛋白的类似物无明显交叉反应，有效降低背景干扰。
• 宽广的检测范围：检测范围为7.8 pg/mL - 500 pg/mL，适用于多种浓度水平的样本检测，无需频繁稀释，提升实验效率。
• 即用型优化组分：试剂盒提供预包被微孔板、高纯度标准品及优化的检测抗体等完整组分，开盒即用，简化实验流程，节省方法建立时间。
• 出色的批次稳定性：严格的生产质控确保不同批次间试剂的高一致性，为长期研究项目的稳定性提供保障。

产品详细信息

产品名称	人白介素-1α ELISA定量试剂盒 (Human IL-1α ELISA Kit)
产品货号	KTE6012-1
反应种属	人 (Human)
检测方法	夹心法ELISA
样本类型	细胞培养上清、血清、血浆及其它生物液体
规格与定价	48 T： 1598.0元 (现货，次日发货) 96 T： 2558.0元 (现货，次日发货)

试剂盒组分

试剂盒包含进行完整ELISA检测所需的所有核心组分：

• 人源 IL-1α 抗体预包被微孔板
• 人源 IL-1α 蛋白标准品
• 人源 IL-1α 检测抗体
• EliKine™ Streptavidin-HRP
• 标准品稀释液、实验缓冲液、HRP底物、反应终止液、洗涤缓冲液
• 封板膜

背景信息

白细胞介素-1α (IL-1α) 是由IL1A基因编码的一种重要的多效性促炎细胞因子，在免疫调节、炎症反应及细胞增殖等生理和病理过程中扮演核心角色。其主要由巨噬细胞、上皮细胞等产生，与TNF等细胞因子具有显著的协同效应。对该细胞因子的精确量化是免疫学和相关疾病研究的关键。

基因ID: 3552 | 蛋白质ID: P01583 (UniProt链接)

重要操作提示

• 请严格按照说明书进行操作，不同批号试剂请勿混用。
• 使用前请将所有试剂室温平衡30分钟，并确保充分混匀。
• 为获得更可靠的结果，建议对样本和标准品进行复孔检测。
• 未使用的板条需立即密封并置于4℃保存。

保存与运输

保存：未开封试剂盒请保存于2-8℃；启用后，请参照说明书分别保存各组分。
运输：全程采用冰袋（蓝冰）低温运输，确保试剂活性。

重要声明：本产品仅限于科研用途，不适用于临床诊断或人体使用。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

The EliKine™ Human Interleukin-1α (IL-1α) ELISA Quantitation Kit is a core tool specifically designed for the accurate quantitation of IL-1α protein levels in human samples. This kit utilizes the classic sandwich ELISA principle and features excellent performance parameters, aiming to provide reliable and highly reproducible data support for cytokine analysis in life science research.

Core Features and Advantages

• High Sensitivity and Specificity: The minimum detectable concentration is as low as 4 pg/mL, ensuring precise capture of low-abundance samples. Rigorously validated to show no significant cross-reactivity with analogs of the target protein, effectively reducing background interference.
• Broad Detection Range: The detection range is 7.8 pg/mL - 500 pg/mL, suitable for samples with various concentration levels, reducing the need for frequent dilutions and improving experimental efficiency.
• Ready-to-Use Optimized Components: The kit provides complete components including pre-coated microplates, high-purity standards, and optimized detection antibodies, ready for immediate use, simplifying the experimental procedure and saving method establishment time.
• Excellent Lot-to-Lot Consistency: Strict production quality control ensures high consistency between different reagent lots, providing reliability for the stability of long-term research projects.

Product Details

Product Name	Human Interleukin-1α ELISA Quantitation Kit (Human IL-1α ELISA Kit)
Catalog Number	KTE6012-1
Reactivity	Human
Detection Method	Sandwich ELISA
Sample Type	Cell culture supernatant, serum, plasma, and other biological fluids
Size & Pricing	48 T: 1598.0 RMB (In stock, ships next day) 96 T: 2558.0 RMB (In stock, ships next day)

Kit Components

The kit contains all core components required for a complete ELISA assay:

• Human IL-1α Antibody Pre-coated Microplate
• Human IL-1α Protein Standard
• Human IL-1α Detection Antibody
• EliKine™ Streptavidin-HRP
• Standard Diluent, Assay Buffer, HRP Substrate, Stop Solution, Wash Buffer
• Plate Sealer

Background

Interleukin-1α (IL-1α) is an important pleiotropic pro-inflammatory cytokine encoded by the IL1A gene, playing a central role in physiological and pathological processes such as immune regulation, inflammatory responses, and cell proliferation. It is primarily produced by macrophages, epithelial cells, etc., and exhibits significant synergistic effects with cytokines like TNF. Precise quantification of this cytokine is crucial for immunology and related disease research.

Gene ID: 3552 | Protein ID: P01583 (UniProt Link)

Important Operational Notes

• Please strictly follow the instructions for use. Do not mix reagents from different lots.
• Before use, equilibrate all reagents to room temperature for 30 minutes and ensure thorough mixing.
• For more reliable results, it is recommended to perform duplicate assays for both samples and standards.
• Unused strips should be sealed immediately and stored at 4°C.

Storage and Shipping

Storage: Unopened kits should be stored at 2-8°C; after opening, please store individual components according to the instructions.
Shipping: Shipped with ice packs (blue ice) to maintain low temperature throughout transit, ensuring reagent activity.

Important Statement: This product is intended for research use only, not for clinical diagnosis or human use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.