人载脂蛋白D ELISA定量试剂盒(Apolipoprote

Human Apolipoprotein D (Apo D) ELISA Kit – KTE3121‑1

产品中文名：人载脂蛋白D ELISA定量试剂盒 (Apolipoprotein D/Apo D)
英文名称：Human Apolipoprotein D/Apo D ELISA Kit
货号：KTE3121-1
产品分类：核心产品 » 细胞分析 » ELISA

适用领域

• 蛋白类研究，特别是神经退行性疾病、代谢紊乱及内分泌相关的机制探索。
• ELISA 应用中的第一线检测平台。
• 样本类型：细胞培养上清、血清、血浆。

技术概述

本套件基于双抗体夹心法，采用特异性抗人 Apo D 抗体进行捕获和检测，实现对人载脂蛋白 D 的高灵敏度定量。检测流程简化为：

1. 抗体预包被于酶标板孔中。
2. 加入待测样本或标准品，Apo D 与固相抗体特异结合。
3. 加入生物素化检测抗体形成二抗复合物。
4. 链霉亲和素‑HRP 结合生物素，实现酶标信号放大。
5. 加入 TMB 底物显色，终止后在 450 nm 读取 OD 值。

关键性能指标

检测范围	0.63 ng/mL – 40 ng/mL
分析灵敏度	0.63 ng/mL
特异性	未检测到显著交叉反应或背景干扰

试剂盒组成

• Human Apo D Microplate
• Human Apo D Standard (lyophilized)
• Assay Buffer (10×)
• Human Apo D Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

使用注意事项

• 不同批号或过期的试剂请勿混用。
• 使用前让所有试剂在室温平衡 30 分钟。
• 实验全程使用一次性吸头，避免交叉污染。
• 未使用的板条请及时放回原包装，密封后于 4 ℃保存。
• 建议使用低速振荡器或每 10 分钟手动轻摇，确保充分混匀。
• 样品与标准建议设置双孔或三孔（复孔）以提升数据可靠性。

保存与运输

保存：未开封试剂盒请避光保存于 2‑8 ℃，有效期 24 个月；启用后请按照说明书分别保存各组分。

运输：采用蓝冰（冷链）包装，确保全程低温。

警示声明

本产品仅供科研使用，不适用于人类或临床诊断。用户须自行确保不侵犯任何专利或许可证，因不当使用导致的法律责任由使用者自行承担。

产品背景简介

Apo D 是约 33 kDa 的糖蛋白，由 APOD 基因编码，主要在大脑和睾丸表达。与 HDL 结合，参与脂质转运及细胞膜的调节。研究表明，Apo D 在神经退行性疾病（如阿尔茨海默病、精神分裂症）、中风模型及雄激素不敏感综合征（AIS）中呈现升高，是重要的生物标志物。本套件为研究人员提供了定量评估该蛋白水平的可靠平台。

规格与报价

规格	单价（人民币）	交货周期
48 T	1,598.0	请咨询
96 T	2,558.0	期货（5 天内发货）

订购方式

如需进一步技术支持或批量采购，请联系销售代表或通过官方渠道提交订单。我们将根据贵司的项目需求提供完整的技术文档与使用指导。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human Apolipoprotein D (Apo D) ELISA Kit – KTE3121‑1

Product Chinese Name: Human Apolipoprotein D ELISA Quantitative Kit (Apolipoprotein D/Apo D)
English Name: Human Apolipoprotein D/Apo D ELISA Kit
Catalog No.: KTE3121-1
Product Category: Core Products » Cell Analysis » ELISA

Applicable Fields

• Protein research, especially investigations into mechanisms of neurodegenerative diseases, metabolic disorders, and endocrine‑related pathways.
• First‑line detection platform for ELISA applications.
• Sample types: cell culture supernatants, serum, plasma.

Technical Overview

This kit is based on a sandwich immunoassay, employing a specific anti‑human Apo D antibody for capture and detection, enabling high‑sensitivity quantification of human Apolipoprotein D. The assay workflow is simplified to:

1. Antibody pre‑coated onto microplate wells.
2. Add samples or standards; Apo D specifically binds to the solid‑phase antibody.
3. Add biotinylated detection antibody to form the secondary antibody complex.
4. Streptavidin‑HRP binds to biotin, enabling signal amplification.
5. Add TMB substrate for color development; after stopping, read OD at 450 nm.

Key Performance Characteristics

Detection Range	0.63 ng/mL – 40 ng/mL
Analytical Sensitivity	0.63 ng/mL
Specificity	No significant cross‑reactivity or background interference detected

Kit Components

• Human Apo D Microplate
• Human Apo D Standard (lyophilized)
• Assay Buffer (10×)
• Human Apo D Detect Antibody (100×)
• Streptavidin‑HRP (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Precautions

• Do not mix reagents from different batches or past expiration.
• Allow all reagents to equilibrate to room temperature for 30 minutes before use.
• Use disposable pipette tips throughout the experiment to avoid cross‑contamination.
• Return unused plate strips to original packaging promptly and store sealed at 4 °C.
• Recommend using a low‑speed shaker or manually invert gently every 10 minutes to ensure thorough mixing.
• Set samples and standards in duplicate or triplicate (replicates) to enhance data reliability.

Storage and Shipping

Storage: Unopened kits should be stored protected from light at 2‑8 °C, shelf life 24 months; after opening, store each component as specified in the manual.

Shipping: Packaged on dry ice (cold chain) to ensure temperature control throughout transit.

Warning Statement

This product is for research use only and is not intended for human or clinical diagnostic applications. Users are responsible for ensuring that no patents or licenses are infringed, and any legal liability arising from misuse is the sole responsibility of the user.

Product Background

Apo D is a ~33 kDa glycoprotein encoded by the APOD gene, predominantly expressed in the brain and testes. It associates with HDL and participates in lipid transport and membrane regulation. Studies have shown that Apo D levels are elevated in neurodegenerative disorders (e.g., Alzheimer’s disease, schizophrenia), stroke models, and androgen insensitivity syndrome (AIS), making it a valuable biomarker. This kit provides researchers with a reliable platform for quantitative assessment of this protein.

Specifications and Pricing

Specification	Unit Price (CNY)	Lead Time
48 T	1,598.0	Please inquire
96 T	2,558.0	Futures (shipment within 5 days)

Ordering Information

For additional technical support or bulk purchasing, please contact a sales representative or submit an order through official channels. We will provide comprehensive technical documentation and usage guidance based on your project requirements.

Note: The above information may change due to product optimizations or upgrades; please consult Yakeyin Company for the latest details.