人血管紧张素原 ELISA定量试剂盒(Angiotensin

产品概览

产品名称：人血管紧张素原 ELISA定量试剂盒 (Angiotensinogen/AGT/SerpinA8)
英文名称：Human Angiotensinogen/AGT/SerpinA8 ELISA Kit
货号：KTE3077-1
产品分类：核心产品 → 细胞分析 → ELISA

适用范围

• 应用领域：蛋白类研究
• 一级应用：ELISA检测
• 检测类型：夹心法 ELISA
• 样本类型：细胞培养上清、血清、血浆

技术优势

• 采用EliKine™ 双抗体夹心设计，灵敏度可达 0.31 ng/mL。
• 检测范围宽：0.31 ng/mL – 20 ng/mL，满足低至中等浓度的定量需求。
• 高特异性：对血管紧张素原类似物无显著交叉反应，背景信号低。
• 标准化试剂配比（10×、20×、100×），简化实验操作。

试剂盒组成

• Human AGT Microplate（预包被抗体板）
• Human AGT Standard (lyophilized) – 多点标准曲线
• Assay Buffer (10×)
• HRP‑conjugated Human AGT Detect Antibody (100×)
• HRP Substrate（TMB）
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

检测原理

本套件基于双抗体夹心法：

1. 微孔板表面预包被特异性抗人血管紧张素原的捕获抗体。
2. 加入待测样品或标准品后，血管紧张素原与捕获抗体形成固相复合物。
3. 加入HRP 标记的检测抗体，实现双抗体桥接。
4. 洗涤去除未结合组分，随后加入TMB底物，HRP 催化产生蓝色信号。
5. 加入终止液后，颜色转为黄色，在 450 nm 读取 OD 值，OD 与浓度呈线性关系。

检测范围与灵敏度

检测范围	0.31 ng/mL – 20 ng/mL
灵敏度（LOD）	0.31 ng/mL

使用建议

• 每个样品建议设置双孔或三孔（复孔），提高结果可靠性。
• 使用一次性吸头，避免交叉污染。
• 实验前让试剂平衡至室温（约30 分钟）。
• 混匀关键步骤建议使用低速振荡器或每10 分钟手动轻摇一次。
• 未使用的板条应立即放回原包装，密闭保存于4 ℃。

存储与运输

• 未开封试剂盒：避光保存于2 °C – 8 °C，保质期 24 个月。
• 启用后各组分请参照说明书分别保存。
• 运输：须使用蓝色冰袋冷链运输。

注意事项 & 警告

• 不同批号或已超过有效期的试剂不得混用。
• 本产品仅供科研使用，禁止用于临床诊断或人类治疗。
• 使用本套件不应违反任何专利或许可证；若因使用产生侵权或其他违规行为，制造商概不负责。

背景信息

血管紧张素原（Angiotensinogen，AGT，又名 SerpinA8）属于丝氨酸蛋白酶抑制剂家族的α‑2球蛋白，主要由肝脏合成并进入血液循环。AGT 是肾素‑血管紧张素系统的核心组成，对血压调节具有重要作用。其裂解产物（血管紧张素‑1、‑2、‑3）在血浆中以一定浓度存在，并显示血管生成抑制活性。AGT 缺失与原发性高血压及肾小管发育不全相关，亦与多种抗血管生成因子（如抗凝血酶、maspin、kallistatin 等）共享调控机制。定量检测 AGT 有助于阐明其在血压调控、肾功能及血管生成中的生理与病理角色。

订购信息

规格	每套含量	单价（元）	交货期
48 T	48 套	1598.00	期货（5 天内发货）
96 T	96 套	2558.00	期货（5 天内发货）

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Human Angiotensinogen ELISA Quantitative Kit (Angiotensinogen/AGT/SerpinA8)
English Name: Human Angiotensinogen/AGT/SerpinA8 ELISA Kit
Catalog No.: KTE3077-1
Product Category: Core Products → Cell Analysis → ELISA

Application Scope

• Application Field: Protein Research
• Primary Application: ELISA Assay
• Assay Type: Sandwich ELISA
• Sample Types: Cell culture supernatant, serum, plasma

Technical Advantages

• Utilizes EliKine™ dual‑antibody sandwich design, with sensitivity as low as 0.31 ng/mL.
• Wide detection range: 0.31 ng/mL – 20 ng/mL, meeting quantitation needs from low to moderate concentrations.
• High specificity: No significant cross‑reactivity with angiotensinogen homologs, low background signal.
• Standardized reagent concentrations (10×, 20×, 100×) simplify assay workflow.

Kit Components

• Human AGT Microplate (pre‑coated antibody plate)
• Human AGT Standard (lyophilized) – multi‑point standard curve
• Assay Buffer (10×)
• HRP‑conjugated Human AGT Detect Antibody (100×)
• HRP Substrate (TMB)
• Stop Solution
• Wash Buffer (20×)
• Plate Covers

Assay Principle

This kit is based on a dual‑antibody sandwich format:

1. The microplate surface is pre‑coated with a capture antibody specific for human angiotensinogen.
2. After adding samples or standards, angiotensinogen binds to the capture antibody forming a solid‑phase complex.
3. Add HRP‑conjugated detection antibody to create the sandwich.
4. Wash to remove unbound material, then add TMB substrate; HRP catalyzes a blue signal.
5. Add stop solution, turning the color yellow; read absorbance at 450 nm, which is linearly proportional to concentration.

Detection Range and Sensitivity

Detection Range	0.31 ng/mL – 20 ng/mL
Sensitivity (LOD)	0.31 ng/mL

Recommendations

• For each sample, use duplicate or triplicate wells to improve data reliability.
• Use disposable pipette tips to avoid cross‑contamination.
• Equilibrate reagents to room temperature (~30 minutes) before the assay.
• For mixing critical steps, use a low‑speed shaker or gently vortex manually every 10 minutes.
• Unused plate strips should be promptly returned to original packaging and stored sealed at 4 °C.

Storage and Shipping

• Unopened kits: store protected from light at 2 °C–8 °C; shelf life 24 months.
• After opening, store each component as instructed in the manual.
• Shipping: use blue ice packs for cold‑chain transport.

Precautions & Warnings

• Do not mix reagents from different batches or past their expiration date.
• This product is for research use only; prohibited for clinical diagnosis or human therapy.
• Use of this kit must not infringe any patents or licenses; the manufacturer assumes no liability for infringement or other violations arising from use.

Background Information

Angiotensinogen (AGT, also known as SerpinA8) belongs to the serine protease inhibitor family α‑2 globulin, primarily synthesized by the liver and released into the circulation. AGT is the central component of the renin‑angiotensin system and plays a crucial role in blood pressure regulation. Its cleavage products (angiotensin‑1, ‑2, ‑3) are present in plasma at defined concentrations and exhibit anti‑angiogenic activity. AGT deficiency is associated with essential hypertension and renal tubular dysgenesis, and shares regulatory mechanisms with various anti‑angiogenic factors (e.g., antithrombin, maspin, kallistatin). Quantitative measurement of AGT aids in elucidating its physiological and pathological roles in blood pressure control, renal function, and angiogenesis.

Ordering Information

Specification	Quantity per Kit	Unit Price (CNY)	Delivery Time
48 T	48 kits	1598.00	In stock (ship within 5 days)
96 T	96 kits	2558.00	In stock (ship within 5 days)

Note: The above information may change due to product optimization or upgrades; please contact Yakein Co. for the latest details.