猪游离甲状腺素 ELISA定量试剂盒(fT4)

Free Thyroxine (fT4) ELISA Kit – 猪游离甲状腺素 ELISA定量试剂盒 (KTE0005‑B10‑1)

产品概述

产品类型：ELISA（竞争法）
产品分类：核心产品 / 细胞分析平台
一级应用：ELISA 应用（蛋白类检测）
检测指标：血清/血浆等生物液体中的游离甲状腺素 (fT4)

技术原理

本套件采用竞争型ELISA技术：微孔板预包被特异性 fT4 抗体，加入待测样本或fT4标准品后，与生物素偶联的fT4竞争有限的结合位点。随后加入Avidin‑HRP形成可视化复合物，通过HRP 底物（A/B）显色并在终止液作用下读取吸光度（OD），以标准曲线定量样本中fT4浓度。

适用物种与样本类型

本试剂盒针对以下物种的抗体已验证交叉反应：

• 人、小鼠、大鼠、山羊、绵羊、狗、兔、牛、猴、鸡、猪

可检测的样本基质包括：

• 血清
• 血浆
• 其他生物液体（经验证的体液）

检测范围与灵敏度

检测范围	4 pmol/L ~ 64 pmol/L
检测限（灵敏度）	4 pmol/L

产品优势

• 极高的灵敏度与特异性，适用于低浓度fT4检测。
• 已通过交叉反应筛查，未检测到显著的类似物干扰。
• 竞争法设计降低基质效应，对血清、血浆等不同基质的适用性良好。

试剂盒组成（每套）

• fT4 抗体预包被微孔板
• fT4 标准品（梯度稀释系列）
• 生物素偶联的 fT4
• Avidin‑HRP
• HRP 底物 A、B（显色剂）
• 反应终止液
• 洗涤缓冲液
• 封板膜

使用说明要点

• 不同批号或已过期的试剂不得混用。
• 使用前请在室温（≈20‑25 ℃）平衡30 分钟。
• 实验全程建议使用一次性吸头，避免交叉污染。
• 未使用的板条请及时回置包装，密封后在4 ℃保存。
• 充分混匀对结果影响显著，推荐低速振荡或每10 分钟手动轻摇。
• 样品与标准品建议设置双孔或复孔（3孔）以确保重复性。

保存与运输

未开封试剂盒：2‑8 ℃冷藏保存。
启用后各组分：请参照说明书中对应的储存条件。
运输条件：使用蓝冰（冷链）包装，确保全程低温。

警示声明

本产品仅供科研使用，不得用于人类临床诊断。使用本试剂盒进行的任何专利或许可证相关活动需自行确保合规，本公司不承担因侵权或违规产生的责任。

产品规格与报价

包装规格	单价（元）	交货周期
48 T	1 598.0	期货（4 天内发货）
96 T	2 558.0	期货（4 天内发货）

技术背景

血清总甲状腺素（T4）与总三碘甲腺原氨酸（T3）大部分（>99 %）与甲状腺素结合球蛋白（TBG）结合，仅有游离形式（fT4、fT3）具备生物活性，直接反映甲状腺功能状态。对fT4的精准定量是评估甲状腺功能、监测疾病进展及药物干预的重要手段。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Free Thyroxine (fT4) ELISA Kit – Porcine Free Thyroxine ELISA Quantitative Kit (KTE0005‑B10‑1)

Product Overview

Product Type: ELISA (competitive assay)
Product Category: Core product / Cell analysis platform
Primary Application: ELISA applications (protein detection)
Analyte: Free thyroxine (fT4) in serum/plasma and other biological fluids

Technical Principle

This kit utilizes a competitive ELISA format: microplate pre‑coated with specific fT4 antibody; upon addition of test samples or fT4 standards, they compete with biotinylated fT4 for a limited number of binding sites. Subsequently, Avidin‑HRP is added to form a visualizable complex, and HRP substrate (A/B) is applied for color development. Absorbance (OD) is measured after addition of stop solution, and fT4 concentration in samples is quantified using a standard curve.

Applicable Species and Sample Types

The antibodies in this kit have been validated for cross‑reactivity with the following species:

• Human, mouse, rat, goat, sheep, dog, rabbit, cattle, monkey, chicken, pig

Detectable sample matrices include:

• Serum
• Plasma
• Other biological fluids (validated specimens)

Assay Range and Sensitivity

Assay Range	4 pmol/L ~ 64 pmol/L
Limit of Detection (Sensitivity)	4 pmol/L

Product Advantages

• Exceptionally high sensitivity and specificity, suitable for low‑concentration fT4 detection.
• Cross‑reactivity screening completed; no significant interference from analogues detected.
• Competitive assay format reduces matrix effects, providing robust performance across serum, plasma, and other matrices.

Kit Components (per package)

• fT4 antibody pre‑coated microplate
• fT4 standard (graded dilution series)
• Biotinylated fT4
• Avidin‑HRP
• HRP substrate A and B (chromogenic reagent)
• Stop solution
• Wash buffer
• Plate sealing film

Key Instructions for Use

• Do not mix reagents of different lot numbers or expired reagents.
• Allow reagents to equilibrate at room temperature (≈20‑25 °C) for 30 minutes before use.
• Use disposable pipette tips throughout the assay to avoid cross‑contamination.
• Return unused strips to their original packaging promptly; seal and store at 4 °C.
• Thorough mixing significantly impacts results; recommend low‑speed shaking or gentle manual inversion every 10 minutes.
• Run samples and standards in duplicate wells or triplicate (3 wells) to ensure reproducibility.

Storage and Transportation

Unopened kits: store refrigerated at 2‑8 °C.
After opening, each component: refer to the user manual for specific storage conditions.
Transportation: ship on dry ice (cold chain) to maintain low temperature throughout.

Warning Statement

This product is for research use only and must not be used for clinical diagnosis in humans. Users are responsible for ensuring compliance with any patents or licenses associated with the use of this kit; the company assumes no liability for infringement or regulatory violations.

Product Specifications and Pricing

Package Size	Unit Price (CNY)	Delivery Lead Time
48 T	1 598.0	Spot (shipment within 4 days)
96 T	2 558.0	Spot (shipment within 4 days)

Technical Background

The majority (>99 %) of total serum thyroxine (T4) and total triiodothyronine (T3) are bound to thyroid‑binding globulin (TBG); only the free forms (fT4, fT3) are biologically active and directly reflect thyroid function. Accurate quantification of fT4 is essential for assessing thyroid status, monitoring disease progression, and evaluating pharmacological interventions.

Note: The above information may change due to product optimizations or upgrades; please contact Yakein Company for the latest details.