牛游离三碘甲状腺原氨酸 ELISA定量试剂盒(fT3)

牛游离三碘甲状腺原氨酸 ELISA定量试剂盒 (fT3) — 核心细胞分析产品

产品概览

英文名称：Free Triiodothyronine (fT3) ELISA Kit
中文名称：牛游离三碘甲状腺原氨酸 ELISA定量试剂盒 (fT3)
货号：KTE0004-B7-1
检测类型：ELISA（竞争法）
适用物种：人、小鼠、大鼠、山羊、绵羊、狗、兔、牛、猴、鸡、猪

关键技术参数

• 检测范围：2 pmol/L – 32 pmol/L
• 灵敏度（检测下限）：2.0 pmol/L
• 样本类型：血清、血浆及其他生物液体
• 检测原理：竞争法——待测 fT3 与生物素偶联 fT3 竞争结合预包被抗体，随后通过 Avidin‑HRP 与底物反应实现定量。

套件组成

每套试剂盒均配备以下材料，确保完整的实验流程：

• fT3 抗体预包被微孔板
• fT3 标准品（梯度浓度）
• 生物素偶联的 fT3
• Avidin‑HRP
• HRP 底物 A 与 B（双底物体系，提升比色信号）
• 反应终止液
• 洗涤缓冲液
• 封板膜（防止污染、保持板面活性）

特点与优势

• 采用 EliKine™ 技术平台，提供极高的灵敏度与特异性。
• 对 fT3 结构类似物的交叉反应经实验验证极低，背景噪声可控。
• 兼容多种动物血清/血浆，使跨物种研究更为便利。
• 双底物设计提升比色呈色均匀性，适用于常规酶标仪读取。

操作提示

• 试剂请勿与不同批号或过期试剂混用。
• 使用前请在室温（≈20‑25℃）平衡 30 分钟。
• 实验过程中建议使用一次性吸头，防止交叉污染。
• 未使用的微孔板请立即放回原包装，密封后置于 4 ℃保存。
• 充分混匀对结果影响显著，推荐低速振荡或每 10 分钟手动轻摇一次。
• 样品与标准品建议设置重复孔（双孔或三孔）以提高数据可靠性。

存储与运输

未开封存储：2 °C – 8 °C
启用后：请参照说明书各组分的具体保存条件。
运输方式：冰袋（蓝冰）冷链运输，确保活性成分稳定。

规格与报价（期货，4 天内发货）

包装规格	数量	单价 (CNY)	交货期
48 T	48 套	1,598.00	期货（4 天内发货）
96 T	96 套	2,558.00	期货（4 天内发货）

使用限制与法律声明

本产品仅供科研使用，**不适用于人类或临床诊断**。请遵守所在地区的专利、许可证及监管要求。因使用本产品而产生的任何专利侵权或违规行为，本公司不承担责任。

技术背景

在甲状腺功能评估中，血清总 T4 与 T3 主要以与甲状腺素结合球蛋白（TBG）结合的形式存在，>99% 的激素被蛋白结合，难以直接反映甲状腺活性。游离甲状腺激素 fT4 与 fT3 为血液中活性形式，不受 TBG 影响，是更直接、敏感的甲状腺功能指标。本套件针对 fT3 的精准定量，为药理、内分泌及动物模型研究提供可靠的数据支撑。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Bovine Free Triiodothyronine (fT3) ELISA Quantitative Kit — Core Cell Analysis Product

Product Overview

English Name: Free Triiodothyronine (fT3) ELISA Kit
Chinese Name: 牛游离三碘甲状腺原氨酸 ELISA定量试剂盒 (fT3)
Catalog No.: KTE0004-B7-1
Assay Type: ELISA (competitive)
Applicable Species: Human, mouse, rat, goat, sheep, dog, rabbit, cattle, monkey, chicken, pig

Key Technical Specifications

• Assay Range: 2 pmol/L – 32 pmol/L
• Sensitivity (LoD): 2.0 pmol/L
• Sample Types: Serum, plasma, and other biological fluids
• Assay Principle: Competitive assay — the target fT3 competes with biotin‑conjugated fT3 for binding to a pre‑coated antibody; subsequent reaction with Avidin‑HRP and substrate yields a quantitative signal.

Kit Components

Each kit includes the following materials to ensure a complete workflow:

• fT3 antibody pre‑coated microplate
• fT3 standards (gradient concentrations)
• Biotin‑conjugated fT3
• Avidin‑HRP
• HRP substrate A and B (dual‑substrate system for enhanced colorimetric signal)
• Reaction stop solution
• Wash buffer
• Plate sealing film (prevents contamination and preserves plate activity)

Features and Advantages

• Utilizes the EliKine™ platform, delivering ultra‑high sensitivity and specificity.
• Cross‑reactivity with fT3 structural analogs experimentally verified to be minimal, with controllable background noise.
• Compatible with a variety of animal sera/plasma, facilitating cross‑species studies.
• Dual‑substrate design improves colorimetric uniformity, suitable for standard microplate readers.

Operating Tips

• Do not mix reagents from different batches or expired reagents.
• Equilibrate at room temperature (≈20‑25 °C) for 30 minutes before use.
• Use disposable tips during the assay to prevent cross‑contamination.
• Return unused microplates to original packaging immediately, seal, and store at 4 °C.
• Thorough mixing significantly impacts results; recommend low‑speed shaking or manual gentle inversion every 10 minutes.
• Set replicate wells (duplicate or triplicate) for samples and standards to enhance data reliability.

Storage and Transportation

Unopened storage: 2 °C – 8 °C
After opening: Refer to the kit manual for specific storage conditions of each component.
Shipping method: Cold‑chain shipping with ice packs (blue ice) to maintain reagent stability.

Specifications and Pricing (Futures, Shipment within 4 Days)

Package Specification	Quantity	Unit Price (CNY)	Delivery Lead Time
48 T	48 sets	1,598.00	Futures (shipped within 4 days)
96 T	96 sets	2,558.00	Futures (shipped within 4 days)

Use Restrictions and Legal Disclaimer

This product is for research use only and **not intended for human or clinical diagnostic applications**. Users must comply with local patents, licenses, and regulatory requirements. The company assumes no liability for any patent infringement or regulatory violations arising from product use.

Technical Background

In thyroid function assessment, total serum T4 and T3 are predominantly bound to thyroxine‑binding globulin (TBG); >99 % of the hormones are protein‑bound, limiting direct reflection of thyroid activity. Free thyroid hormones fT4 and fT3 represent the biologically active fractions in blood, unaffected by TBG, and serve as more direct and sensitive thyroid function markers. This kit provides precise quantification of fT3, delivering reliable data for pharmacology, endocrinology, and animal model studies.

Note: The information above may change due to product optimizations or updates; please contact Yakein for the latest details.