IL4, IL-4, IL-4B,BCGF1, BCGF-1

重组人白介素-4 (IL-4) 蛋白

产品编号: PRP2017

英文名称: Human IL-4 protein

产品类型: 无标签重组细胞因子

产品概述

本品为一种高纯度重组人白介素-4（Interleukin-4, IL-4）蛋白，通过人胚胎肾细胞（HEK293）表达系统制备，氨基酸序列涵盖Met 1至Ser153（UniProt ID: P05112）。该产品经严格质控，纯度≥95%，以其高生物活性及低内毒素水平（<1.0 EU/μg）为细胞培养与免疫学研究提供可靠支持。

技术参数

• 种属来源: 人源 (Human)
• 表达宿主: 人胚胎肾细胞 (HEK293 cells)
• 蛋白长度: 153个氨基酸
• 预测分子量: 15.4 kDa
• SDS-PAGE表观分子量: ≈18 kDa (还原条件)
• 纯度: ≥95% (SDS-PAGE分析)
• 内毒素: <1.0 EU/μg (LAL法检测)
• 产品形式: 冻干粉 (冻干缓冲液: 无菌PBS, pH 7.4)
• 标签: 无标签

产品特点与优势

• 采用HEK293真核表达系统，有助于维持蛋白天然构象与功能。
• 无标签设计，尽可能减少对下游实验的潜在干扰。
• 极低的内毒素水平，适用于对内毒素敏感的细胞实验。
• 提供多种规格选择，满足不同实验规模的需求。

应用方向 (研究用途)

• B淋巴细胞与T淋巴细胞的体外增殖与分化研究
• Th2型免疫应答及相关细胞因子网络的机制探索
• 过敏性炎症（如哮喘）及肿瘤微环境的体外模型建立
• IgE类别转换的分子机制研究

使用建议

复溶: 建议使用提供的复溶缓冲液（Reconstitution Buffer）进行复溶，浓度不低于100 µg/mL。复溶前请先离心产品管。

保存: 冻干粉应置于-20℃以下干燥环境保存。复溶后可在4℃稳定保存2-7天；长期保存请分装并于-20℃以下冻存，并建议添加载体蛋白（如0.1% HSA或BSA）以避免反复冻融带来的活性损失。

运输: 常温运输。

背景信息

白介素-4（IL-4）是一种多效性的抗炎细胞因子，在免疫调节中扮演着核心角色。它最初被鉴定为一种T细胞来源的B细胞生长因子（BCGF-1）。IL-4通过驱动初始CD4+ T细胞向Th2表型分化、诱导B细胞发生IgE类别转换、上调VCAM-1表达以及促进嗜酸性粒细胞迁移等机制，在哮喘、肿瘤和HIV感染等多种疾病的病理过程中发挥着重要作用。其基因多态性也与哮喘易感性相关。

规格与价格

警告: 本产品仅限于科研用途，不适用于临床诊断、治疗或其他人体用途。

基因ID: 3565 | UniProt ID: P05112

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-4 (IL-4) Protein

Catalog Number: PRP2017

Product Name: Human IL-4 protein

Product Type: Tag-free Recombinant Cytokine

Product Overview

This product is a highly pure recombinant Human Interleukin-4 (IL-4) protein, produced using a human embryonic kidney cell (HEK293) expression system. The amino acid sequence spans Met 1 to Ser153 (UniProt ID: P05112). Rigorously quality-controlled, this product boasts a purity of ≥95%, high biological activity, and low endotoxin levels (<1.0 EU/μg), providing reliable support for cell culture and immunology research.

Technical Specifications

• Species: Human
• Expression Host: Human Embryonic Kidney Cells (HEK293 cells)
• Protein Length: 153 amino acids
• Predicted Molecular Weight: 15.4 kDa
• Apparent Molecular Weight (SDS-PAGE): ≈18 kDa (under reducing conditions)
• Purity: ≥95% (analyzed by SDS-PAGE)
• Endotoxin Level: <1.0 EU/μg (determined by LAL assay)
• Formulation: Lyophilized powder (Lyophilized from a sterile PBS buffer, pH 7.4)
• Tag: Tag-free

Features & Advantages

• Produced using a HEK293 eukaryotic expression system, facilitating the maintenance of the protein's native conformation and function.
• Tag-free design minimizes potential interference in downstream experiments.
• Extremely low endotoxin levels, suitable for cell-based assays sensitive to endotoxin.
• Available in various sizes to meet different experimental scale requirements.

Applications (Research Use Only)

• In vitro proliferation and differentiation studies of B lymphocytes and T lymphocytes
• Investigation of Th2 immune responses and related cytokine networks
• Establishment of in vitro models for allergic inflammation (e.g., asthma) and tumor microenvironment studies
• Studies on the molecular mechanisms of IgE class switching

Usage Instructions

Reconstitution: It is recommended to reconstitute the lyophilized powder using the provided Reconstitution Buffer to a concentration not less than 100 µg/mL. Centrifuge the vial briefly before reconstitution.

Storage: The lyophilized powder should be stored dry at or below -20°C. Upon reconstitution, the product can be stored at 4°C for 2-7 days. For long-term storage, aliquot and freeze at or below -20°C. Adding a carrier protein (e.g., 0.1% HSA or BSA) is recommended to prevent activity loss due to repeated freeze-thaw cycles.

Shipping: Shipped at ambient temperature.

Background

Interleukin-4 (IL-4) is a pleiotropic anti-inflammatory cytokine that plays a central role in immune regulation. It was initially identified as a T cell-derived B-cell growth factor (BCGF-1). IL-4 is critically involved in the pathophysiology of various diseases such as asthma, cancer, and HIV infection by driving the differentiation of naive CD4+ T cells into a Th2 phenotype, inducing IgE class switching in B cells, upregulating VCAM-1 expression, and promoting eosinophil migration, among other mechanisms. Genetic polymorphisms in the IL-4 gene are also associated with susceptibility to asthma.

Specifications & Pricing

Warning: This product is for research use only. It is not intended for diagnostic, therapeutic, or any other human use.

Gene ID: 3565 | UniProt ID: P05112

Note: The information provided above is subject to change due to factors such as product optimization and upgrades. Please contact Yakeyin Company for specific details.