Hepatocyte growth factor, Hepa

重组人肝细胞生长因子 (HGF)，无动物源表达

产品概述

Human HGF Protein, His tag (Animal Free) 是一种由HEK293细胞表达的重组蛋白片段，对应人源HGF (UniProt #P14210) 的Gln32-Ser728氨基酸区域。该蛋白无标签、高纯度（>90%），经严格质控，适用于细胞培养等研究应用。

产品信息

• 产品名称：重组人肝细胞生长因子，His-标签（无动物源）（HGF）
• 英文名称：Human HGF Protein, His tag (Animal Free)
• 货号：PRP1950
• 种属：人源
• 表达宿主：HEK293细胞（无动物源成分）
• 分子量：80.48 kDa
• 蛋白长度：697个氨基酸
• 纯度：>90%（SDS-PAGE检测）
• 内毒素水平：＜1 EU/µg（LAL法测定）

产品特点

• 无动物源表达系统，降低外源因子风险
• 高纯度与低内毒素，适用于敏感细胞实验
• 冻干粉形式提供，稳定性高，便于运输与储存

使用说明

• 复溶建议：推荐使用配套复溶缓冲液，浓度不低于100 µg/mL，后续可根据实验需求进一步稀释
• 保存条件：冻干粉建议保存于-20℃以下干燥环境；复溶后可在4℃保存2-7天，长期保存请置于-20℃以下，建议添加载体蛋白（如0.1% BSA）并分装避免反复冻融
• 注意事项：使用前请离心，本品仅供研究使用

背景信息

肝细胞生长因子（Hepatocyte Growth Factor, HGF）是一种来源于间充质细胞的多功能细胞因子，通过激活c-Met受体酪氨酸激酶信号通路，在血管生成、肿瘤发生及组织再生等过程中发挥核心作用。HGF最初以无活性单链形式分泌，经蛋白酶水解后形成具有生物活性的异二聚体结构。

规格与价格

运输条件：冰袋运输（蓝冰）

警告：本品仅供研究使用，不适用于人体或临床诊断。使用前请查阅相关文献并遵守所在机构的规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Hepatocyte Growth Factor (HGF), Animal-Free Expression

Product Overview

Human HGF Protein, His tag (Animal Free) is a recombinant protein fragment expressed in HEK293 cells, corresponding to the amino acid region Gln32-Ser728 of human HGF (UniProt #P14210). This protein is tag-free, of high purity (>90%), undergoes strict quality control, and is suitable for research applications such as cell culture.

Product Information

• Product Name: Recombinant Human Hepatocyte Growth Factor, His-tag (Animal Free) (HGF)
• English Name: Human HGF Protein, His tag (Animal Free)
• Catalog Number: PRP1950
• Species: Human
• Expression Host: HEK293 cells (Animal-Free Components)
• Molecular Weight: 80.48 kDa
• Protein Length: 697 amino acids
• Purity: >90% (analyzed by SDS-PAGE)
• Endotoxin Level: ＜1 EU/µg (determined by LAL method)

Product Features

• Animal-free expression system, reducing the risk of exogenous factors
• High purity and low endotoxin, suitable for sensitive cell experiments
• Supplied as lyophilized powder, high stability, convenient for transportation and storage

Usage Instructions

• Reconstitution Recommendation: It is recommended to use the accompanying reconstitution buffer, with a concentration not less than 100 µg/mL; further dilution can be performed according to experimental needs.
• Storage Conditions: Lyophilized powder should be stored dry at -20°C or below; after reconstitution, it can be stored at 4°C for 2-7 days. For long-term storage, please store at -20°C or below, and it is recommended to add a carrier protein (e.g., 0.1% BSA) and aliquot to avoid repeated freeze-thaw cycles.
• Precautions: Centrifuge before use. This product is for research use only.

Background Information

Hepatocyte Growth Factor (HGF) is a multifunctional cytokine derived from mesenchymal cells. By activating the c-Met receptor tyrosine kinase signaling pathway, it plays a central role in processes such as angiogenesis, tumorigenesis, and tissue regeneration. HGF is initially secreted as an inactive single-chain form and is proteolytically cleaved to form the biologically active heterodimeric structure.

Specifications & Pricing

Shipping Conditions: Shipped with ice packs (blue ice)

Warning: This product is for research use only, not intended for human use or clinical diagnosis. Please review relevant literature and adhere to your institution's regulations before use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.