C16orf77, IL34, 重组蛋白,细胞因子

重组人白介素-34蛋白 (His标签，无动物源)

货号：PRP1918

产品名称：Human IL-34 Protein, His tag (Animal-Free)

产品概述

本品为采用大肠杆菌表达系统重组表达的无动物源人源白介素-34 (IL-34) 蛋白，携带His标签。其氨基酸序列覆盖人IL-34的Asn21至Pro242区域。该产品经专为细胞培养及生物医学研究设计，具有高纯度与高生物活性，适用于体外细胞功能研究、信号通路分析及受体相互作用探索。

关键特性

• 高纯度：经SDS-PAGE验证，纯度 > 98%
• 低内毒素：LAL法检测，内毒素含量 < 0.1 EU/µg
• 高生物活性：ED₅₀ < 30 pg/mL（基于人外周血单核细胞增殖试验）
• 无动物源成分：生产全程无动物源组分，降低外源因子风险
• 冻干粉形态：提供便捷的储存与运输条件

产品信息

种属来源	人
表达宿主	大肠杆菌
分子量	约 30 kDa
标签	His标签
缓冲体系	冻干前为无菌PBS, pH 8.0

生物活性验证

经功能实验验证，本品可特异性诱导人外周血单核细胞 (PBMC) 增殖，半数有效浓度 (ED₅₀) 低于 30 pg/mL，表明其具有优异的生物学活性，适用于对信号通路灵敏度要求较高的细胞实验。

使用建议

• 复溶：建议使用配套复溶缓冲液 (Reconstitution Buffer) 复溶至浓度不低于 100 µg/mL，后续可根据实验需求进一步稀释。
• 保存：冻干粉可在 -20°C 下稳定保存1年；复溶后于 2-8°C 可保存1周。如需长期保存，建议在含稳定剂（如0.1% BSA）的缓冲液中稀释后分装，并存储于 -20°C 或 -80°C，避免反复冻融。
• 实验操作：使用前请短暂离心，以确保产品完全沉于管底。

背景知识

白细胞介素-34 (IL-34) 是一种重要的细胞因子，是集落刺激因子1受体 (CSF-1R) 的关键配体。它由骨髓细胞、上皮细胞、神经元等多种细胞类型分泌，通过激活下游信号通路参与调控细胞存活、增殖、迁移及炎症因子表达，在单核吞噬系统的发育与稳态维持中扮演核心角色。该蛋白也被称为 C16orf77，其UniProt编号为 Q6ZMJ4。

规格与价格

规格	价格（人民币）	货期
5 µg	588	现货，次日发货
20 µg	1498	现货，次日发货
100 µg	3389	请咨询
1 mg	请咨询	请咨询

运输条件：本品采用蓝冰冰袋运输，以保障产品稳定性。

警告：本品仅限于科研使用，不适用于临床诊断、治疗或其他人体用途。使用者应确保其应用不侵犯现有专利或许可，制造商不对此类侵权或违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interleukin-34 Protein (His‑tag, Animal‑Free)

Catalog No.: PRP1918

Product Name: Human IL‑34 Protein, His tag (Animal‑Free)

Product Overview

This product is a recombinant, animal‑free human interleukin‑34 (IL‑34) protein expressed in an Escherichia coli system and carries an N‑terminal His‑tag. The amino‑acid sequence spans residues Asn21 to Pro242 of human IL‑34. Designed specifically for cell‑culture and biomedical research, the protein exhibits high purity and robust biological activity, making it suitable for in‑vitro cell‑function assays, signaling‑pathway analyses, and receptor‑interaction studies.

Key Features

• High Purity: SDS‑PAGE confirmed purity > 98%
• Low Endotoxin: LAL assay, endotoxin < 0.1 EU/µg
• High Biological Activity: ED₅₀ < 30 pg/mL (based on human peripheral blood mononuclear cell proliferation assay)
• Animal‑Free Composition: Produced without any animal‑derived components, reducing risk of extraneous factors
• Lyophilized Form: Facilitates convenient storage and shipment

Product Information

Species Source	Human
Expression Host	Escherichia coli
Molecular Weight	≈ 30 kDa
Tag	His‑tag
Buffer System	Sterile PBS, pH 8.0 (pre‑lyophilization)

Biological Activity Validation

Functional assays demonstrate that this product specifically induces proliferation of human peripheral blood mononuclear cells (PBMCs) with a half‑maximal effective dose (ED₅₀) below 30 pg/mL, confirming its superior bioactivity and suitability for cell‑based experiments requiring high‑sensitivity signaling read‑outs.

Recommended Usage

• Reconstitution: Use the supplied reconstitution buffer to achieve a concentration of at least 100 µg/mL; further dilutions can be prepared according to experimental needs.
• Storage: Lyophilized powder is stable at –20 °C for up to 1 year. After reconstitution, store at 2–8 °C for up to 1 week. For long‑term storage, aliquot into a stabilizing buffer (e.g., 0.1 % BSA) and keep at –20 °C or –80 °C, avoiding repeated freeze‑thaw cycles.
• Handling: Briefly centrifuge the vial before use to ensure the product settles at the bottom of the tube.

Background

Interleukin‑34 (IL‑34) is a pivotal cytokine and a principal ligand of the colony‑stimulating factor‑1 receptor (CSF‑1R). Secreted by bone‑marrow cells, epithelial cells, neurons, and other cell types, IL‑34 activates downstream signaling cascades that regulate cell survival, proliferation, migration, and inflammatory‑gene expression. It plays a central role in the development and homeostasis of the mononuclear phagocyte system. The protein is also known as C16orf77 and is cataloged in UniProt under accession number Q6ZMJ4.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock, next‑day shipping
20 µg	1498	In stock, next‑day shipping
100 µg	3389	Contact for quotation
1 mg	Contact for quotation	Contact for quotation

Shipping Conditions: The product is shipped with blue ice packs to maintain stability.

Warning: This product is intended for research use only and is not suitable for clinical diagnosis, therapy, or any human applications. Users must ensure that their use does not infringe existing patents or licenses; the manufacturer assumes no liability for any infringement or regulatory violations.

Note: Information is subject to change with product updates or optimizations. Please contact Yakoin Co., Ltd. for the latest details.