interleukin 6, IFN-β2, B-Cell

重组小鼠白介素6 (IL-6) 蛋白

英文名称：Mouse IL-6 Protein
货号：PRP1229
产品类型：高纯度重组细胞因子

产品概述

本品为大肠杆菌表达的重组小鼠白介素6 (IL-6) 蛋白，氨基酸序列源自UniProt登录号P08505 (Phe25-Thr211)。该蛋白是一种重要的多功能细胞因子，在免疫调节、炎症反应及细胞分化中扮演核心角色，尤其对B细胞终末分化为免疫球蛋白分泌细胞具有关键作用。

技术参数

• 种属来源：小鼠
• 表达宿主：大肠杆菌表达系统
• 蛋白长度：187个氨基酸
• 预测分子量：21.9 kDa
• 纯度：经SDS-PAGE分析，大于99%
• 内毒素水平：LAL法检测，每微克蛋白低于0.1 EU
• 产品形式：冻干粉（冻干缓冲液：20 mM Tris, 150 mM NaCl, pH 8.5）
• 标签：无标签（Null）

活性说明

本品生物学活性检测仍在进行中。建议使用者根据具体实验体系验证其功能活性。

使用建议

• 使用前请离心，以确保产品完全收集于管底。
• 推荐使用配套复溶缓冲液进行复溶，浓度不低于100 µg/mL，后续可根据实验需要进行稀释。
• 为保持蛋白稳定性，复溶后可于4℃短期保存（2-7天）；长期保存请置于-20℃以下，并建议添加载体蛋白（如0.1% HSA或BSA），分装保存以避免反复冻融。

保存与运输

• 保存条件：冻干粉建议保存于-20℃以下干燥环境。
• 运输条件：冰袋（蓝冰）运输，以保证产品稳定性。

背景信息

白介素6 (IL-6) 是一种多效性细胞因子，兼具促炎和免疫调节功能。它在机体应对感染、损伤后的反应中起重要作用，并可调节急性期反应。小鼠IL-6可由成纤维细胞、活化T细胞、单核/巨噬细胞及内皮细胞产生，作用于多种细胞类型（如B细胞、T细胞、肝细胞等），参与B细胞分化、浆细胞生长及肝细胞急性期蛋白诱导等过程。

基因ID：16193
UniProt登录号：P08505

规格与价格

警告：本产品仅限于科研用途，不适用于人类或临床诊断。使用者应确保其应用不侵犯任何专利或许可协议，厂家对因使用本品可能造成的专利侵权或其他违规行为不承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interleukin-6 (IL-6) Protein

English Name: Mouse IL-6 Protein
Catalog Number: PRP1229
Product Type: High Purity Recombinant Cytokine

Product Overview

This product is a recombinant mouse Interleukin-6 (IL-6) protein expressed in E. coli, with an amino acid sequence derived from UniProt accession number P08505 (Phe25-Thr211). This protein is an important multifunctional cytokine that plays a central role in immune regulation, inflammatory responses, and cell differentiation, and is particularly critical for the terminal differentiation of B cells into immunoglobulin-secreting cells.

Technical Parameters

• Species Source: Mouse
• Expression Host: E. coli Expression System
• Protein Length: 187 amino acids
• Predicted Molecular Weight: 21.9 kDa
• Purity: >99% as determined by SDS-PAGE analysis
• Endotoxin Level: <0.1 EU per µg protein, determined by LAL method
• Product Form: Lyophilized powder (Lyophilization buffer: 20 mM Tris, 150 mM NaCl, pH 8.5)
• Tag: Tag-free (Null)

Activity Note

The biological activity testing of this product is still ongoing. Users are advised to validate its functional activity within their specific experimental systems.

Usage Recommendations

• Centrifuge the tube briefly before opening to ensure the product is fully collected at the bottom.
• It is recommended to reconstitute the product to a concentration no less than 100 µg/mL using the provided reconstitution buffer, and it can be further diluted according to experimental needs.
• For short-term storage (2-7 days), the reconstituted product can be kept at 4°C. For long-term storage, store below -20°C and consider adding a carrier protein (e.g., 0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Storage and Shipping

• Storage Condition: The lyophilized powder should be stored desiccated below -20°C.
• Shipping Condition: Shipped on blue ice to ensure product stability.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine with both pro-inflammatory and immunoregulatory functions. It plays a significant role in the body's response to infection and injury and can modulate the acute phase response. Mouse IL-6 can be produced by fibroblasts, activated T cells, monocytes/macrophages, and endothelial cells. It acts on various cell types (such as B cells, T cells, hepatocytes, etc.) and is involved in processes including B cell differentiation, plasma cell growth, and the induction of acute phase proteins in hepatocytes.

Gene ID: 16193
UniProt Accession: P08505

Specifications and Pricing

Warning: This product is intended for research use only and is not for human or clinical diagnostic use. The user should ensure that its use does not infringe upon any patents or licensing agreements. The manufacturer assumes no liability for patent infringement or other violations resulting from the use of this product.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult YaKeYin Company for specific details.