fas ligand, soluble Fas Ligand

重组人Fas配体蛋白，His-SUMO标签（无动物源）

产品概述

本品为高纯度、高活性重组人源FasL蛋白，采用无动物源表达系统生产，严格质量控制，适用于细胞培养及相关生命科学研究。该产品通过大肠杆菌表达系统制备，具有优异的生物活性和稳定性。

产品详情

• 产品名称: Human FasL Protein, His-SUMO tag (Animal-Free)
• 货号: PRP1212
• 蛋白序列: 人源FasL Protein (Gln130-Leu281) (UniProt ID: P48023-1)
• 标签类型: His-SUMO
• 种属来源: 人
• 表达宿主: 大肠杆菌（无动物源表达系统）
• 分子量: 29.51 kDa
• 蛋白长度: 152个氨基酸

生物活性

经测试，本品能够有效诱导Jurkat细胞凋亡，半数有效浓度（ED₅₀）＜1 ng/mL。重组人FasL蛋白的比活性＞1×10⁶ IU/mg，显示出卓越的生物学功能。

纯度与质量控制

• 纯度: ＞98%（SDS-PAGE检测）
• 内毒素水平: ＜0.1 EU/μg（LAL法测定）

产品形式与复溶建议

本品为冻干粉形式，冻干缓冲液为无菌PBS（pH 8.0）。使用前请离心，推荐使用配套复溶缓冲液复溶至浓度不低于100 µg/mL。可根据实验需求进一步稀释。

保存与运输条件

• 保存条件: 冻干粉可在-20°C保存1年；复溶后于2-8°C可保存1周；稀释后分装保存于-20°C或-80°C可稳定3-6个月。请避免反复冻融。
• 运输条件: 冰袋运输（蓝冰）

背景信息

Fas配体（FasL），也称为sFasL、TNFSF6或CD95L，是一种17.34 kDa的肿瘤坏死因子超家族成员，由152个氨基酸残基组成。FasL主要在淋巴组织中表达，并可分泌至血液中。通过与受体TNFRSF6/FAS结合，可诱导细胞凋亡信号传导，参与细胞毒性T细胞介导的凋亡、自然杀伤细胞介导的凋亡及T细胞发育等关键生物学过程。

产品规格与价格

注意事项

警告：本产品仅供研究使用，不适用于人类或临床诊断。使用前请阅读相关文献并了解所在地区的专利和许可证要求。对于因使用本产品可能引起的专利侵权或其他违规行为，本公司不承担任何责任。

别名：FASL, FasL, fas ligand, soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178

基因ID：356

UniProt链接：P48023

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Fas Ligand Protein, His-SUMO Tag (Animal-Free)

Product Overview

This product is a highly pure and highly active recombinant human FasL protein, produced using an animal-free expression system, with strict quality control. It is suitable for cell culture and related life science research. This product is prepared using an E. coli expression system, offering excellent bioactivity and stability.

Product Details

• Product Name: Human FasL Protein, His-SUMO tag (Animal-Free)
• Catalog Number: PRP1212
• Protein Sequence: Human FasL Protein (Gln130-Leu281) (UniProt ID: P48023-1)
• Tag Type: His-SUMO
• Species Source: Human
• Expression Host: E. coli (Animal-Free Expression System)
• Molecular Weight: 29.51 kDa
• Protein Length: 152 amino acids

Biological Activity

Testing has shown that this product can effectively induce apoptosis in Jurkat cells, with a half maximal effective concentration (ED₅₀) <1 ng/mL. The specific activity of the recombinant human FasL protein is >1×10⁶ IU/mg, demonstrating exceptional biological function.

Purity and Quality Control

• Purity: >98% (as determined by SDS-PAGE)
• Endotoxin Level: <0.1 EU/μg (determined by LAL method)

Product Form and Reconstitution Recommendation

This product is supplied as a lyophilized powder, lyophilized from sterile PBS (pH 8.0). Centrifuge the vial before use. It is recommended to reconstitute it to a concentration not less than 100 µg/mL using the provided reconstitution buffer. It can be further diluted according to experimental needs.

Storage and Shipping Conditions

• Storage Conditions: The lyophilized powder can be stored at -20°C for 1 year; after reconstitution, it can be stored at 2-8°C for 1 week; after dilution and aliquoting, it can be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze-thaw cycles.
• Shipping Conditions: Shipped on ice packs (blue ice)

Background Information

Fas ligand (FasL), also known as sFasL, TNFSF6, or CD95L, is a 17.34 kDa member of the tumor necrosis factor superfamily, composed of 152 amino acid residues. FasL is primarily expressed in lymphoid tissues and can be secreted into the blood. By binding to its receptor TNFRSF6/FAS, it can induce apoptotic signaling cascades, participating in key biological processes such as cytotoxic T cell-mediated apoptosis, natural killer cell-mediated apoptosis, and T-cell development.

Specifications and Pricing

Precautions

Warning: This product is for research use only, not intended for human or clinical diagnostic use. Please read relevant literature and understand the patent and licensing requirements in your region before use. Our company assumes no responsibility for any patent infringement or other violations that may arise from the use of this product.

Aliases: FASL, FasL, fas ligand, soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178

Gene ID: 356

UniProt Link:P48023

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.