Epithelial Neutrophil Activati

产品概述

产品名称：重组小鼠趋化因子配体5（CXCL5），His‑标签（无动物源）
商品编号：PRP1190
别名：ENA‑78、AMCF、CXCL6、GCP‑2、LIX、Scyb5 等

本品为来源于小鼠（Mus musculus）的趋化因子 CXCL5（氨基酸序列 Val45‑Ala118），通过大肠杆菌表达体系获得的重组蛋白，适用于细胞因子及蛋白类研发平台。

核心优势

• 高纯度：采用 SDS‑PAGE 鉴定，纯度 ≥98%。
• 低内毒素：通过 LAL 法测定，每 µg 蛋白内毒素含量 < 0.1 EU，适合对内毒素敏感的细胞实验。
• 活性评估：对转染人 CXCR2 的 BaF3 细胞具有化学吸引活性，ED₅₀ < 100 ng/mL。
• 动物源‑Free：表达宿主为大肠杆菌，避免动物来源成分的潜在干扰。

技术规格

推荐使用浓度与复溶指南

• 使用配套的 Reconstitution Buffer（无菌 PBS, pH 7.4）进行复溶，建议浓度不低于 100 µg/mL。
• 复溶后如需稀释，请根据实验需求使用 0.1 % BSA、10 % FBS 或 5 % HSA 等载体蛋白/稳定剂，以提升蛋白稳定性。
• 使用前请先离心除去可能的沉淀，避免对细胞实验造成影响。

典型应用

本蛋白可用于以下细胞培养相关研究：

• 趋化活性及 CXCR2 受体信号通路的功能验证。
• 炎症模型中中性粒细胞的招募研究。
• 血管生成相关的体外实验。
• IL‑1、TNFα 或 IFNγ 诱导的炎症反应机制探索。

规格与报价

存储与安全注意事项

• 冻干粉请储存于‑20 °C，避免光照。
• 复溶后严禁反复冻融；分装后建议储存于‑20 °C 或‑80 °C。
• 使用前务必离心去除可能的颗粒。
• 本品为研究试剂，仅限实验室科研使用，不适用于临床诊断或人用。

法律声明

本产品仅供科研用途。使用本品进行的任何实验不应违反现行专利、许可证或其他法律规定。公司不承担因专利侵权或其他违规行为产生的任何责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Recombinant Mouse Chemokine (C-X-C motif) Ligand 5 (CXCL5), His‑Tag (Animal-Free)
Catalog Number: PRP1190
Synonyms: ENA‑78, AMCF, CXCL6, GCP‑2, LIX, Scyb5, etc.

This product is a recombinant protein derived from mouse (Mus musculus) chemokine CXCL5 (amino acid sequence Val45‑Ala118), produced in an E. coli expression system. It is suitable for cytokine and protein research platforms.

Key Features

• High Purity: Determined by SDS‑PAGE analysis to be ≥98%.
• Low Endotoxin: Less than 0.1 EU per µg of protein as measured by LAL assay, suitable for cell culture experiments sensitive to endotoxin.
• Activity Assay: Exhibits chemotactic activity towards BaF3 cells transfected with human CXCR2, with an ED₅₀ < 100 ng/mL.
• Animal-Free: Expressed in an E. coli host, avoiding potential interference from animal-derived components.

Technical Specifications

Recommended Usage Concentration & Reconstitution Guide

• Reconstitute using the provided Reconstitution Buffer (sterile PBS, pH 7.4). A final concentration of at least 100 µg/mL is recommended.
• For further dilution after reconstitution, use carrier proteins/stabilizers such as 0.1 % BSA, 10 % FBS, or 5 % HSA based on experimental requirements to enhance protein stability.
• Centrifuge briefly before use to remove any potential precipitates and avoid interference in cell-based assays.

Typical Applications

This protein can be used for the following cell culture-related studies:

• Functional validation of chemotactic activity and CXCR2 receptor signaling pathways.
• Research on neutrophil recruitment in inflammation models.
• In vitro experiments related to angiogenesis.
• Investigation of inflammatory response mechanisms induced by IL‑1, TNFα, or IFNγ.

Specifications & Pricing

Storage & Safety Notes

• Store the lyophilized powder at -20 °C, protected from light.
• Avoid repeated freeze-thaw cycles after reconstitution; aliquot and store at -20 °C or -80 °C.
• Always centrifuge before use to remove any potential particulates.
• This product is a research reagent intended for laboratory research use only. Not for use in diagnostic procedures or human use.

Legal Disclaimer

This product is intended for research purposes only. Any experimentation using this product shall not violate existing patents, licenses, or other legal regulations. The company assumes no liability for patent infringement or other violations arising from the use of this product.

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.