MIP-1b: Macrophage Inflammator

重组小鼠 CCL4 蛋白 (MIP-1β, His 标签, 无动物源)

货号: PRP1189

产品概述: 本品为一种高纯度、无动物源表达的重组小鼠巨噬细胞炎症蛋白-1β (MIP-1β/CCL4)，采用大肠杆菌表达系统生产，并带有His标签以便于纯化与检测。专为细胞培养及相关免疫学研究设计，具有卓越的生物活性和批次稳定性。

产品特性

• 高纯度: 经SDS-PAGE验证，纯度超过98%，确保实验结果可靠。
• 低内毒素: 采用LAL法检测，内毒素含量低于0.1 EU/µg，最大限度地减少对细胞实验的非特异性干扰。
• 生物活性验证: 经功能实验验证，在20.0-200.0 ng/mL浓度范围内可有效趋化人PBMC（注：实验结果可能因供体差异而有所不同）。
• 无动物源成分: 在生产过程中不添加任何动物源成分，降低了病毒污染风险，更适合对纯度要求高的研究应用。

产品信息

使用说明

复溶建议: 开盖前请先离心。推荐使用产品配套的复溶缓冲液进行复溶，初始浓度不低于100 µg/mL，后续可根据实验需求进行稀释。

保存条件: 冻干粉可在-20°C下稳定保存1年。复溶后，于2-8°C可保存1周。若在含有载体蛋白（如0.1% BSA）的缓冲液中进一步稀释并分装，于-20°C或-80°C可保存3-6个月，建议避免反复冻融。

背景知识

巨噬细胞炎症蛋白-1β (MIP-1β/CCL4) 是一种由C-C基序趋化因子配体4基因编码的重要细胞因子，分子量约为7.66 kDa。它主要由中性粒细胞、单核细胞、淋巴细胞等多种免疫细胞分泌，在炎症反应和免疫调节中发挥关键作用，例如参与白细胞募集和激活。

规格与价格

运输: 本品采用冰袋（蓝冰）运输，以确保产品在途中的稳定性。

重要提示: 本产品仅用于科学研究，不适用于人类或临床诊断。使用前请查阅相关文献，确保您的应用不违反任何专利或许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse CCL4 Protein (MIP‑1β, His‑tag, Animal‑component free)

Product Number: PRP1189

Product Overview: This is a high‑purity, animal‑component‑free recombinant mouse macrophage inflammatory protein‑1β (MIP‑1β/CCL4) produced in an Escherichia coli expression system and bearing a His‑tag for purification and detection. It is designed for cell‑culture and related immunology studies, offering excellent bioactivity and batch‑to‑batch consistency.

Product Features

• High Purity: Verified by SDS‑PAGE with purity >98%, ensuring reliable experimental results.
• Low Endotoxin: Determined by LAL assay, endotoxin level <0.1 EU/µg, minimizing nonspecific interference in cell assays.
• Bioactivity Confirmed: Functional assays demonstrate effective chemotaxis of human PBMCs in the 20.0–200.0 ng/mL range (note: results may vary with donor).
• Animal‑Component Free: No animal‑derived materials are used during production, reducing viral contamination risk and suited for high‑purity research applications.

Product Information

Usage Instructions

Reconstitution Recommendation: Centrifuge before opening. Reconstitute with the supplied buffer to an initial concentration ≥100 µg/mL; subsequent dilutions can be made according to experimental needs.

Storage Conditions: Lyophilized powder is stable at –20 °C for 1 year. After reconstitution, store at 2–8 °C for up to 1 week. If further diluted and aliquoted in a buffer containing carrier protein (e.g., 0.1 % BSA), store at –20 °C or –80 °C for 3–6 months; avoid repeated freeze‑thaw cycles.

Background

Macrophage inflammatory protein‑1β (MIP‑1β/CCL4) is an important cytokine encoded by the C‑C motif chemokine ligand 4 (CCL4) gene, with a molecular weight of ~7.66 kDa. It is secreted by neutrophils, monocytes, lymphocytes and other immune cells, playing a key role in inflammatory responses and immune regulation, such as leukocyte recruitment and activation.

Specifications and Pricing

Shipping: The product is shipped with ice packs (blue ice) to maintain stability during transit.

Important Notice: This product is for scientific research only and is not intended for human or clinical diagnostic use. Please consult the literature before use to ensure your application does not infringe any patents or licenses. Note: The above information may change due to product optimization or updates; please contact Yakein Co. for the latest details.