Interleukin-12 subunit beta, I

重组小鼠白介素-12 p40，His-标签（无动物源）

产品概述

重组小鼠Interleukin-12 subunit beta (IL-12 p40)蛋白，货号PRP1173，是一种通过大肠杆菌表达系统重组表达的高纯度细胞因子。该蛋白为冻干粉剂型，不含动物源成分，适用于多种细胞培养及免疫学研究场景。

关键特性

• 高纯度：经SDS-PAGE验证，纯度超过98%，确保实验的一致性与可靠性。
• 低内毒素：LAL法检测内毒素含量＜0.1 EU/µg，极大降低对细胞实验的干扰。
• 无动物源表达：采用无动物源重组技术生产，减少外源因子污染风险。
• 生物活性验证：经功能试验验证，可有效诱导富含T细胞的PBMC增殖，ED₅₀为0.3 ng/mL。

产品信息

应用方向

本品可广泛用于T细胞增殖、NK细胞功能研究、免疫调节机制探索以及相关药物筛选等体外实验。

使用建议

• 复溶前请先离心，建议使用配套复溶缓冲液，浓度不低于100 µg/mL。
• 可根据实验需求进一步稀释，推荐在含稳定剂（如0.1% BSA）的缓冲体系中进行。
• 避免反复冻融，建议分装保存。

保存与运输

• 保存条件：冻干粉-20°C可保存1年；复溶后于2-8°C可保存1周；稀释分装后-20°C或-80°C可保存3-6个月。
• 运输方式：冰袋运输（蓝冰），保障产品稳定性。

背景介绍

白细胞介素12（IL-12）是一种重要的异源二聚体促炎细胞因子，由p35和p40两个亚基构成。其中IL-12 p40不仅在IL-12的形成中起关键作用，还能以单体或同源二聚体形式存在，参与多种免疫反应的调节过程。

规格与价格

注意：本品仅供科研使用，不用于人体或临床诊断。使用者应自行负责确保其应用不侵犯专利或许可证限制。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interleukin-12 p40, His‑tag (Animal‑Free)

Product Overview

Recombinant mouse Interleukin‑12 subunit beta (IL‑12 p40) protein, catalog number PRP1173, is a high‑purity cytokine expressed via an Escherichia coli expression system. The protein is supplied as a lyophilized powder, free of animal‑derived components, and is suitable for a variety of cell‑culture and immunological research applications.

Key Features

• High Purity: Verified by SDS‑PAGE with purity >98%, ensuring experimental consistency and reliability.
• Low Endotoxin: LAL assay detects endotoxin levels <0.1 EU/µg, greatly minimizing interference with cell‑based assays.
• Animal‑Free Expression: Produced using animal‑free recombinant technology, reducing the risk of exogenous factor contamination.
• Biological Activity Verified: Functional assays demonstrate effective induction of T‑cell‑rich PBMC proliferation, with an ED₅₀ of 0.3 ng/mL.

Product Information

Applications

This product can be widely used for T‑cell proliferation, NK cell functional studies, exploration of immune‑regulatory mechanisms, and related drug screening in vitro.

Usage Recommendations

• Prior to reconstitution, centrifuge the lyophilized powder; it is recommended to use the provided reconstitution buffer, achieving a concentration of ≥100 µg/mL.
• Further dilute as needed; recommended to perform dilutions in a buffer containing stabilizers (e.g., 0.1 % BSA).
• Avoid repeated freeze‑thaw cycles; aliquot for storage.

Storage and Shipping

• Storage Conditions: Lyophilized powder stable at –20 °C for 1 year; post‑reconstitution stable at 2‑8 °C for 1 week; diluted aliquots stable at –20 °C or –80 °C for 3‑6 months.
• Shipping Method: Shipped on ice packs (blue ice) to ensure product stability.

Background

Interleukin‑12 (IL‑12) is a key heterodimeric pro‑inflammatory cytokine composed of p35 and p40 subunits. IL‑12 p40 not only plays a critical role in IL‑12 assembly but also exists as a monomer or homodimer, participating in the regulation of various immune responses.

Specifications and Pricing

Note: This product is intended for research use only and is not for human or clinical diagnostic applications. Users are responsible for ensuring that their use does not infringe any patents or licensing restrictions.

Note: The above information may change due to product optimization or upgrades; please contact Yakeyin Company for the most current details.