P600, 重组小鼠白介素-13, His-标签（无动物源）

Mouse IL-13 Protein, His tag (Animal-Free) - PRP1159

重组小鼠白介素-13（IL-13）是一种由大肠杆菌表达系统重组制备的高纯度细胞因子蛋白，C端带有6×His标签，全程无动物源成分参与，适用于各类细胞培养与免疫学研究。

产品详情

• 产品名称: P600, 重组小鼠白介素-13, His-标签（无动物源）
• 英文名称: Mouse IL-13 Protein, His tag (Animal-Free)
• 货号: PRP1159
• 种属来源: 小鼠
• 表达宿主: 大肠杆菌
• 蛋白长度: 约 13.06 kDa（SDS-PAGE 显示约 11 kDa，还原条件）
• 纯度: > 95%（SDS-PAGE 验证）
• 内毒素水平: < 0.1 EU/µg（LAL法测定）

生物学活性

本品通过诱导 TF-1 细胞增殖实验验证其活性，ED₅₀ <4 ng/mL，显示高生物活性与稳定性。

产品形态与复溶建议

本品为冻干粉形式，经 0.2 µm 过滤并冻干于 1X PBS（pH 7.4）缓冲体系中。推荐使用配套复溶缓冲液（Reconstitution Buffer）复溶至浓度不低于 100 µg/mL，后续可根据实验需要进行稀释。

保存与运输

• 保存条件: 冻干粉可于 -20°C 保存1年；复溶后于 2°C–8°C 可稳定保存1周；稀释后可分装存于 -20°C 或 -80°C，避免反复冻融。
• 运输方式: 冰袋运输（蓝冰）

应用领域

IL-13 主要由 Th2 细胞、肥大细胞、嗜碱性粒细胞等分泌，在调节B细胞功能、炎症反应及免疫应答中发挥重要作用。本品适用于：

• 细胞增殖与分化研究
• 免疫信号通路分析
• 体外细胞模型构建
• 蛋白互作与受体研究

注意事项

使用前请离心。本品为研究用途，不适用于人体或临床诊断。使用前请查阅相关文献与法规，确保符合实验伦理与知识产权要求。

规格与价格

更多产品信息或定制需求，欢迎联系我们获取专业支持。

基因编号: 不适用
UniProt 编号: P20109 (Q6EBC2)

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse IL-13 Protein, His tag (Animal-Free) - PRP1159

Recombinant mouse interleukin‑13 (IL‑13) is a high‑purity cytokine protein produced by a recombinant Escherichia coli expression system, featuring a C‑terminal 6×His tag. The entire production process is free of animal‑derived components, making it suitable for a wide range of cell culture and immunological studies.

Product Details

• Product Name: P600, Recombinant Mouse Interleukin‑13, His‑tag (Animal‑Free)
• English Name: Mouse IL‑13 Protein, His tag (Animal‑Free)
• Catalog No.: PRP1159
• Species Source: Mouse
• Expression Host: Escherichia coli
• Protein Length: Approximately 13.06 kDa (appears ~11 kDa on SDS‑PAGE under reducing conditions)
• Purity: > 95% (verified by SDS‑PAGE)
• Endotoxin Level: < 0.1 EU/µg (LAL assay)

Biological Activity

The product’s activity was confirmed by inducing proliferation of TF‑1 cells, with an ED₅₀ <4 ng/mL, demonstrating high bioactivity and stability.

Product Form and Reconstitution Recommendations

The product is supplied as a lyophilized powder, lyophilized from a 0.2 µm‑filtered 1X PBS (pH 7.4) solution. It is recommended to reconstitute with the provided Reconstitution Buffer to a concentration of at least 100 µg/mL, after which it can be diluted as needed for experiments.

Storage and Shipping

• Storage Conditions: Lyophilized powder is stable at –20 °C for up to 1 year; after reconstitution, it can be kept at 2 °C–8 °C for up to 1 week; aliquoted, diluted samples should be stored at –20 °C or –80 °C, avoiding repeated freeze‑thaw cycles.
• Shipping Method: Ice‑pack (blue ice) shipment

Application Areas

IL‑13 is primarily secreted by Th2 cells, mast cells, basophils, and other cell types, playing a critical role in regulating B‑cell function, inflammatory responses, and immune signaling. This product is suitable for:

• Cell proliferation and differentiation studies
• Immune signaling pathway analysis
• In vitro cellular model construction
• Protein‑protein interaction and receptor studies

Precautions

Centrifuge before use. This product is for research purposes only and is not intended for human or clinical diagnostic use. Please consult relevant literature and regulations to ensure compliance with experimental ethics and intellectual property requirements.

Specifications and Pricing

For more product information or custom requests, please contact us for professional support.

Gene ID: N/A
UniProt ID: P20109 (Q6EBC2)

Note: The above information may change due to product optimization or other factors; please consult Yakoin Co. for the most current details.