IFNB1, Type I Interferon, 重组蛋白

重组人干扰素-β1a (IFN-β1a), His标签 (无动物源)

货号：PRP1053 | 产品类型：高纯度细胞因子重组蛋白

产品概述

本产品为采用大肠杆菌表达系统重组表达的人源干扰素-β1a (IFN-β1a) 蛋白，其氨基酸序列对应于UniProt登录号P01574所记录的Met30-Asn187片段。该蛋白在C末端融合有6×His标签，便于纯化与检测。生产过程采用无动物源工艺，并通过严格的质控流程，确保产品的高纯度与高生物活性，适用于对动物源成分敏感的细胞培养及功能研究。

关键特性

• 高纯度与一致性：经SDS-PAGE验证，纯度超过98%，在还原条件下迁移至约15 kDa，与理论分子量20.84 kDa相符。
• 卓越的生物活性：经功能验证，其诱导HeLa细胞凋亡的ED₅₀ < 15 ng/mL；在TF-1细胞中诱导细胞毒性的ED₅₀ < 0.1 ng/mL。比活性测定结果>1 × 10⁷ IU/毫克。
• 低内毒素水平：采用LAL法检测，每1 μg蛋白质中内毒素含量小于0.1 EU，满足高灵敏度细胞实验需求。
• 无动物源生产：全程无动物源成分，降低外源因子引入风险，提升实验安全性。

产品规格与保存

本品为经0.2 µm过滤后冻干的白色粉末，冻干缓冲液为1X PBS (pH 8.0)。

复溶建议：开盖前请短暂离心。推荐使用配套复溶缓冲液将冻干粉溶解至浓度不低于100 µg/mL，后续可根据实验体系进行稀释。为保持长期稳定性，建议在含有稳定剂（如0.1% BSA、10% FBS或5% HSA）的缓冲液中进一步稀释并分装保存。

保存条件：冻干粉可在-20°C下稳定保存1年。复溶后于2-8°C可保存1周；分装后于-20°C或-80°C可保存3-6个月，请避免反复冻融。

生物学背景

干扰素-β1a (IFN-β1a) 是I型干扰素家族的重要成员，是一种由166个氨基酸组成的细胞因子。它通过结合细胞表面的I型干扰素受体，激活JAK-STAT信号通路，进而调控免疫细胞的增殖、分化与活化，在先天性与适应性免疫应答中发挥核心作用。该蛋白在病毒感染应答、细胞生长调控及免疫调节研究中具有广泛应用。

订购信息

运输条件

所有订单均采用冰袋（蓝冰）保温运输，以确保产品在运输过程中的稳定性。

使用注意：本产品仅限用于科学研究，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或法规许可。

参考文献序列： UniProt: P01574 (IFNB1)

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Interferon-β1a (IFN-β1a), His-tag (Animal‑Free)

Product No.: PRP1053 | Product Type: High‑purity cytokine recombinant protein

Product Overview

This product is a recombinant human interferon‑β1a (IFN‑β1a) expressed using an Escherichia coli expression system. The amino‑acid sequence corresponds to the Met30‑Asn187 fragment recorded under UniProt accession P01574. The protein carries a C‑terminal 6×His tag to facilitate purification and detection. The manufacturing process is animal‑origin‑free and follows stringent quality‑control procedures to ensure high purity and high biological activity, suitable for cell culture and functional studies that are sensitive to animal‑derived components.

Key Characteristics

• High purity and consistency: SDS‑PAGE analysis confirms purity >98%; under reducing conditions the protein migrates at ~15 kDa, consistent with the theoretical molecular weight of 20.84 kDa.
• Excellent biological activity: Functional assays show an ED₅₀ for inducing apoptosis in HeLa cells < 15 ng/mL; cytotoxicity in TF‑1 cells with an ED₅₀ < 0.1 ng/mL. Specific activity >1 × 10⁷ IU/mg.
• Low endotoxin level: LAL testing indicates endotoxin < 0.1 EU per µg protein, meeting the requirements of highly sensitive cellular assays.
• Animal‑origin‑free production: No animal‑derived reagents are used throughout, reducing the risk of exogenous factor introduction and enhancing experimental safety.

Specification and Storage

The product is a white lyophilized powder filtered through a 0.2 µm membrane, lyophilized in 1× PBS (pH 8.0).

Reconstitution recommendation: Briefly centrifuge before opening. Dissolve the lyophilized powder in the supplied reconstitution buffer to a concentration of ≥100 µg/mL; subsequent dilutions can be made according to experimental requirements. For long‑term stability, further dilute in a buffer containing stabilizers (e.g., 0.1 % BSA, 10 % FBS, or 5 % HSA) and aliquot.

Storage conditions: Lyophilized powder is stable at –20 °C for up to 1 year. After reconstitution, store at 2–8 °C for up to 1 week; aliquoted samples can be stored at –20 °C or –80 °C for 3–6 months. Avoid repeated freeze‑thaw cycles.

Biological Background

Interferon‑β1a (IFN‑β1a) is a key member of the type I interferon family, a cytokine composed of 166 amino acids. It binds the type I interferon receptor on the cell surface, activates the JAK‑STAT signaling pathway, and thereby regulates proliferation, differentiation, and activation of immune cells. IFN‑β1a plays a central role in innate and adaptive immune responses and is widely used in studies of viral infection, cell growth control, and immune modulation.

Ordering Information

Shipping Conditions

All orders are shipped with a blue ice pack to maintain temperature stability during transport.

Usage notice: This product is intended for scientific research only and is not for human or clinical diagnostic use. Users must ensure that its application does not infringe any relevant patents or regulatory restrictions.

Reference sequence: UniProt: P01574 (IFNB1)

Note: Information is subject to change due to product optimization or updates; please contact Yakeyin Co. for the latest details.