IL-1RA细胞因子,重组人白细胞介素-1受体拮抗剂,重组蛋

IL-1RA 细胞因子（重组人白细胞介素‑1受体拮抗剂）

产品概述

本产品为重组人IL‑1RA蛋白（His标签），采用 E.coli 表达系统生产，采用动物来源‑free工艺，提供高纯度冻干粉形式，适用于细胞培养及细胞因子相关的基础研究。

关键特性 & 优势

• 高纯度：使用 SDS‑PAGE 检测，蛋白纯度 > 98%。
• 低内毒素水平：LAL 检测每 µg 蛋白的内毒素含量 < 0.1 EU，满足细胞实验对内毒素的严格要求。
• 功能活性：在 D10.G4.1 细胞系中对 IL‑1α‑依赖性增殖的抑制活性 ED₅₀ < 50 ng/mL。
• His标签便于后续的亲和纯化与检测。
• 提供专用无菌 PBS（pH 7.4）复溶缓冲液，保证复溶过程的完整性。

技术规格

可选规格 & 价格

推荐应用

本产品适用于以下细胞层面的研究：

• IL‑1 受体拮抗剂活性测定（如 D10.G4.1 细胞增殖抑制实验）。
• 炎症信号通路的调控研究，包括 NF‑κB、MAPK 等下游效应。
• 细胞因子网络分析与体外细胞因子抑制模型。
• 共培养体系中对单核/巨噬细胞活化的负向调节实验。

使用说明 & 注意事项

1. 使用前请先离心去除可能的颗粒。
2. 采用本公司提供的无菌 PBS 缓冲液复溶，确保浓度不低于 100 µg/mL。
3. 如需进一步稀释，请依据实验需求使用含载体蛋白或稳定剂的缓冲体系（如 0.1 % BSA、10 % FBS、5 % HSA），并在稀释后分装保存。
4. 避免反复冻融循环，以免影响活性。

保存与运输

冻干蛋白：-20 °C 保存，保质期 1 年。
复溶后：2 °C‑8 °C 保存，最长可存 1 周。若在含载体蛋白的缓冲液中二次分装，建议在 -20 °C 或 -80 °C 保存 3‑6 个月。
运输方式：使用蓝冰（冰袋）冷链运输，确保全程低温。

合规与免责声明

本品仅供 研究使用，不适用于人类治疗或临床诊断。使用本产品进行的任何专利或许可证相关行为，均由用户自行承担风险，供应方不承担相应责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

IL-1RA Cytokine (Recombinant Human Interleukin‑1 Receptor Antagonist)

Product Overview

This product is Recombinant Human IL‑1RA Protein (His Tag), produced using an E. coli expression system with an animal‑free process. It is provided as a high-purity lyophilized powder, suitable for cell culture and basic research related to cytokines.

Key Features & Advantages

• High Purity: Protein purity > 98% as determined by SDS‑PAGE.
• Low Endotoxin Level: Endotoxin content < 0.1 EU per µg of protein as measured by LAL assay, meeting the stringent requirements for cell-based experiments.
• Functional Activity: ED₅₀ < 50 ng/mL for the inhibition of IL‑1α‑dependent proliferation in the D10.G4.1 cell line.
• His Tag facilitates downstream affinity purification and detection.
• Supplied with dedicated sterile PBS (pH 7.4) reconstitution buffer to ensure the integrity of the reconstitution process.

Specifications

Available Sizes & Pricing

Recommended Applications

This product is suitable for the following cell-based studies:

• IL‑1 receptor antagonist activity assays (e.g., D10.G4.1 cell proliferation inhibition assay).
• Regulation studies of inflammatory signaling pathways, including downstream effects such as NF‑κB and MAPK.
• Cytokine network analysis and in vitro cytokine inhibition models.
• Negative regulation experiments on monocyte/macrophage activation in co-culture systems.

Usage Instructions & Notes

1. Briefly centrifuge the vial prior to use to remove any potential particulates.
2. Reconstitute using the provided sterile PBS buffer to a concentration not less than 100 µg/mL.
3. For further dilution, use a buffer system containing a carrier protein or stabilizer (e.g., 0.1 % BSA, 10 % FBS, 5 % HSA) according to experimental needs, and aliquot after dilution.
4. Avoid repeated freeze-thaw cycles to preserve activity.

Storage & Shipping

Lyophilized Protein: Store at -20 °C. Stable for 1 year.
After Reconstitution: Store at 2 °C‑8 °C for up to 1 week. If aliquoted in a buffer containing a carrier protein, store at -20 °C or -80 °C for 3‑6 months.
Shipping Condition: Shipped with blue ice (ice pack) to ensure a cold chain throughout transit.

Compliance & Disclaimer

This product is intended for Research Use Only, not for human therapeutic use or clinical diagnosis. Any actions related to patents or licenses undertaken using this product are the sole responsibility of the user; the supplier assumes no liability.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.