SCF细胞因子,重组人干细胞因子,重组蛋白,E.coli,H

重组人干细胞因子 (SCF)，His标签，无动物源

货号：PRP1905

英文名称：Human SCF Protein, His tag (Animal-Free)

产品描述

本产品为重组人干细胞因子（SCF），采用无动物源成分的大肠杆菌表达系统生产，C端带有6×His标签。该蛋白经严格纯化与质量控制，适用于细胞培养等研究应用，尤其在与造血干细胞增殖和分化相关的实验中表现卓越。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 > 98%
• 低内毒素：LAL法检测，内毒素含量 < 0.1 EU/μg
• 高生物活性：比活性 > 5 × 10⁵ IU/mg，ED₅₀ < 5 ng/mL（TF-1细胞增殖试验）
• 无动物源成分：生产过程中未使用任何动物源性原料，降低外源因子风险

技术参数

• 种属来源：人
• 表达系统：大肠杆菌（E.coli）
• 蛋白长度：165个氨基酸（Thr25-Ala189）
• 预测分子量：18.6 kDa（实际观测约19.4 kDa）
• 标签：C端6×His
• 蛋白ID：P21583（UniProt）

应用建议

本品推荐用于体外细胞培养研究，特别是在造血干细胞、祖细胞扩增及协同细胞因子作用等实验中。复溶时建议使用配套复溶缓冲液，浓度不低于100 μg/mL，以避免蛋白聚集。后续可根据实验体系进行稀释。

保存与运输

• 保存条件：冻干粉-20℃可保存1年；复溶后2-8℃可保存1周；稀释后（如加入0.1% BSA）可分装存于-20℃/-80℃，避免反复冻融。
• 运输条件：冰袋（蓝冰）运输

规格与价格

规格	价格（元）	货期
5 μg	588	现货，次日发货
20 μg	1498	现货，次日发货
100 μg	3016	现货，次日发货
1 mg	请咨询	请咨询

注意事项

使用前请离心。本品为研究用途，不用于人体或临床诊断。使用前请查阅相关文献及法规，确保符合实验要求。

背景信息

干细胞因子（SCF）是c-kit受体的配体，可调节多种造血生长因子（如G-CSF、GM-CSF、IL-3），促进造血干细胞的增殖、分化和存活。其在造血、黑色素生成和生殖细胞发育中具有重要作用。

别名：KIT ligand, KL-1, MGF, SF, SLF, DCUA, DFNA69, FPH2, FPHH, SHEP7

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Stem Cell Factor (SCF), His‑tag, Animal‑Free

Product Number: PRP1905

English Name: Human SCF Protein, His tag (Animal‑Free)

Product Description

This product is recombinant human stem cell factor (SCF) produced in an animal‑free Escherichia coli expression system with a C‑terminal 6×His tag. The protein is rigorously purified and quality‑controlled, making it suitable for cell‑culture and related research applications, particularly excelling in assays involving hematopoietic stem cell proliferation and differentiation.

Key Features

• High Purity: SDS‑PAGE analysis shows purity > 98 %
• Low Endotoxin: LAL assay, endotoxin < 0.1 EU/µg
• High Biological Activity: Specific activity > 5 × 10⁵ IU/mg, ED₅₀ < 5 ng/mL (TF‑1 cell proliferation assay)
• Animal‑Free: No animal‑derived raw materials used in production, reducing risk of exogenous factors

Technical Specifications

• Species Origin: Human
• Expression System: Escherichia coli (E. coli)
• Protein Length: 165 amino acids (Thr25‑Ala189)
• Theoretical Molecular Weight: 18.6 kDa (observed ≈ 19.4 kDa)
• Tag: C‑terminal 6×His
• Protein ID: P21583 (UniProt)

Recommended Applications

This product is recommended for in vitro cell‑culture studies, especially experiments involving hematopoietic stem/progenitor cell expansion and synergistic cytokine activities. Reconstitution should be performed with the supplied buffer at a concentration ≥ 100 µg/mL to minimize aggregation; subsequent dilutions can be made according to the specific assay requirements.

Storage and Shipping

• Storage Conditions: Lyophilized powder – –20 ℃ for up to 1 year; reconstituted – 2–8 ℃ for up to 1 week; diluted (e.g., with 0.1 % BSA) can be aliquoted and stored at –20 ℃/‑80 ℃, avoiding repeated freeze‑thaw cycles.
• Shipping Conditions: Ice packs (blue ice) during transport

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock, next‑day shipment
20 µg	1498	In stock, next‑day shipment
100 µg	3016	In stock, next‑day shipment
1 mg	Please inquire	Please inquire

Precautions

Centrifuge before use. This product is for research purposes only and is not intended for human or clinical diagnostic use. Consult relevant literature and regulations to ensure compliance with your experimental requirements.

Background Information

Stem cell factor (SCF) is the ligand for the c‑kit receptor and can modulate various hematopoietic growth factors (e.g., G‑CSF, GM‑CSF, IL‑3), promoting proliferation, differentiation, and survival of hematopoietic stem cells. It plays critical roles in hematopoiesis, melanogenesis, and germ cell development.

Synonyms: KIT ligand, KL‑1, MGF, SF, SLF, DCUA, DFNA69, FPH2, FPHH, SHEP7

Note: The above information may change due to product optimization or upgrades; please contact Yakeyin Co. for the most current details.