IL-19细胞因子,重组小鼠白介素-19,重组蛋白,E.co

重组小鼠白介素-19 (IL-19) 蛋白，无动物源，His标签

货号：PRP1194

产品类型：高纯度重组细胞因子

种属来源：小鼠

产品描述

本产品为通过大肠杆菌表达系统重组表达的小鼠白细胞介素-19 (Interleukin-19, IL-19) 蛋白，不含动物源成分，C末端带有His标签。该蛋白序列对应于小鼠IL-19的Leu25-Ala176氨基酸片段，经纯化后以冻干粉形式提供，旨在为免疫学与细胞功能研究提供高纯度的活性工具蛋白。

关键特性

• 高纯度：经SDS-PAGE检测，纯度 >98%
• 低内毒素：LAL法检测，内毒素含量 <0.1 EU/µg
• 生物活性验证：通过在人IL-20Rα/β转染的BaF3细胞中诱导增殖的生物学活性测定，其ED₅₀ <0.6 ng/mL
• 无动物源表达：在生产过程中未使用任何动物源性原料，降低外源因子干扰风险

技术参数

>152个氨基酸（预测）

表达宿主	大肠杆菌 (E.coli)
蛋白长度
分子量	约 17-25 kDa（还原条件下SDS-PAGE）
标签类型	C端His标签
产品形式	冻干粉（冻干缓冲液：无菌PBS, pH 8.0）

生物活性背景

白细胞介素-19（IL-19）属于IL-10细胞因子家族，是一种预测分子量约为35.8 kDa的抗炎细胞因子。它在免疫调节中发挥重要作用，包括：

• 促进Th2型T细胞应答，支持抗炎淋巴细胞表型；
• 抑制Th1型T细胞应答与IFNγ分泌；
• 上调外周血单个核细胞（PBMCs）中IL-10的表达；
• 抑制B细胞产生IgG。

这些特性使IL-19在研究炎症反应、自身免疫及感染性疾病模型中具有重要价值。

使用建议

复溶方法：推荐使用本品配套的复溶缓冲液进行复溶，复溶浓度建议不低于100 µg/mL，后续可根据实验体系需求使用含载体蛋白（如0.1% BSA、10% FBS、5% HSA）的缓冲液进行进一步稀释。

注意事项：使用前请离心。避免反复冻融，复溶后分装保存有助于维持蛋白稳定性。

保存与运输

• 保存条件：冻干粉可在-20°C下稳定保存1年；复溶后于2-8°C可保存1周；稀释并分装后于-20°C或-80°C可保存3-6个月。
• 运输条件：蓝冰冰袋运输，确保蛋白活性不受损。

规格与价格

规格	价格（元）	货期
5 µg	588	现货，次日发货
20 µg	1498	现货，次日发货
100 µg	4789	请咨询
1 mg	请咨询	请咨询

重要说明：本品仅供科研使用，不适用于临床诊断、治疗或其他人体用途。使用者应确保其应用不违反相关专利或许可规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interleukin-19 (IL-19) Protein, Animal-Free, His-Tagged

Catalog Number: PRP1194

Product Type: High Purity Recombinant Cytokine

Species: Mouse

Product Description

This product is a recombinant Mouse Interleukin-19 (IL-19) protein expressed in an E. coli expression system, free of animal-derived components, with a C-terminal His tag. The protein sequence corresponds to the Leu25-Ala176 fragment of mouse IL-19. It is provided as a lyophilized powder after purification and is intended to provide a high-purity, active tool protein for immunology and cellular function research.

Key Features

• High Purity: >98% purity as determined by SDS-PAGE.
• Low Endotoxin: Endotoxin level <0.1 EU/µg as measured by LAL assay.
• Bioactivity Validated: Biological activity determined by inducing proliferation in BaF3 cells transfected with human IL-20Rα/β; ED₅₀ <0.6 ng/mL.
• Animal-Free Expression: No animal-derived materials are used in the production process, reducing the risk of exogenous factor interference.

Technical Parameters

Expression Host	Escherichia coli (E. coli)
Protein Length	>152 amino acids (predicted)
Molecular Weight	Approximately 17-25 kDa (under reducing conditions, SDS-PAGE)
Tag Type	C-terminal His Tag
Form	Lyophilized powder (Lyophilization buffer: sterile PBS, pH 8.0)

Biological Activity Background

Interleukin-19 (IL-19), a member of the IL-10 cytokine family, is an anti-inflammatory cytokine with a predicted molecular weight of approximately 35.8 kDa. It plays important roles in immune regulation, including:

• Promoting Th2-type T cell responses, supporting an anti-inflammatory lymphocyte phenotype;
• Inhibiting Th1-type T cell responses and IFNγ secretion;
• Upregulating IL-10 expression in peripheral blood mononuclear cells (PBMCs);
• Inhibiting IgG production by B cells.

These properties make IL-19 valuable for studying inflammatory responses, autoimmune diseases, and infectious disease models.

Usage Recommendations

Reconstitution: It is recommended to reconstitute the lyophilized powder using the provided reconstitution buffer to a concentration no less than 100 µg/mL. Further dilution can be performed using buffers containing carrier proteins (e.g., 0.1% BSA, 10% FBS, 5% HSA) as required by the experimental system.

Note: Centrifuge the vial briefly before use. Avoid repeated freeze-thaw cycles; aliquot after reconstitution to maintain protein stability.

Storage and Shipping

• Storage: The lyophilized powder is stable for 1 year when stored at -20°C. After reconstitution, it can be stored at 2-8°C for 1 week. Diluted and aliquoted samples can be stored at -20°C or -80°C for 3-6 months.
• Shipping: Shipped on blue ice packs to ensure protein activity is preserved.

Specifications and Pricing

Specification	Price (RMB)	Lead Time
5 µg	588	In stock, ships next day
20 µg	1498	In stock, ships next day
100 µg	4789	Please inquire
1 mg	Please inquire	Please inquire

Important Note: This product is for research use only. It is not intended for diagnostic, therapeutic, or any other human use. Users should ensure their application does not violate any relevant patents or licensing agreements.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.