FGF-2细胞因子,重组小鼠/大鼠成纤维细胞生长因子-2,重

Mouse/Rat FGF-2 Protein, His Tag (Animal-Free) | PRP1150

本品为高纯度、动物源无添加的重组小鼠/大鼠成纤维细胞生长因子-2（FGF-2），携带His标签，适用于多种细胞培养与发育生物学研究应用。

产品概述

Mouse/Rat FGF-2 Protein（PRP1150）是一种通过大肠杆菌表达系统重组表达的功能性细胞因子。该蛋白来源于小鼠/大鼠FGF-2氨基酸序列（Ala11-Ser154），并在N端融合6×His标签，便于后续纯化与检测。

关键特性

• 高纯度：经SDS-PAGE检测，纯度大于98%
• 低内毒素：LAL法检测，每1μg蛋白质中EU含量小于0.1
• 高生物活性：ED50＜1.5 ng/mL，可显著诱导3T3细胞增殖
• 无动物源成分：全程无动物源组分添加，降低外源因子污染风险

技术参数

• 种属来源：小鼠/大鼠
• 表达宿主：大肠杆菌（E.coli）
• 蛋白长度：144个氨基酸
• 分子量：17.2 kDa（计算值），SDS-PAGE中迁移约为19 kDa
• 标签：N端6×His
• 蛋白形态：冻干粉，含0.01% sarkosyl，pH 8.0 PBS缓冲体系

生物活性验证

通过3T3细胞增殖试验测定，该重组FGF-2蛋白表现出优异的生物学活性，其特异性活性大于1×10⁶ IU/mg。

使用建议

• 复溶方法：建议使用无菌ddH₂O复溶，浓度不低于100 μg/mL，室温静置20分钟以上，避免振荡
• 保存条件：冻干粉-20℃可保存1年；复溶后于2–8℃可保存1周；稀释后可分装存于-20℃或-80℃，避免反复冻融
• 运输条件：冰袋（蓝冰）运输

应用领域

• 干细胞与祖细胞增殖与分化研究
• 细胞培养与类器官构建
• 组织修复与伤口愈合机制研究
• 肿瘤生物学与血管生成研究

背景信息

FGF-2（碱性成纤维细胞生长因子，又称HBGF-2或Prostatropin）是一种多效性细胞因子，在胚胎发育、组织修复、神经生成及肿瘤发生等多种生理和病理过程中发挥重要作用。其在多种细胞类型中均有表达，具有广泛的科研与转化价值。

规格与价格

规格	价格（元）	货期
20 μg	588	现货，次日发货
100 μg	1498	现货，次日发货
500 μg	3998	请咨询
1 mg	请咨询	请咨询

注意：本品仅供科研使用，不适用于人体或临床诊断。用户应自行确保在使用过程中不侵犯任何专利或许可证要求。

如需更多信息或定制要求，请随时与我们联系。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Mouse/Rat FGF-2 Protein, His Tag (Animal-Free) | PRP1150

This product is a high‑purity, animal‑origin‑free recombinant mouse/rat fibroblast growth factor‑2 (FGF‑2) with a His tag, suitable for a variety of cell culture and developmental biology research applications.

Product Overview

Mouse/Rat FGF-2 Protein (PRP1150) is a functional cytokine recombinantly expressed via an Escherichia coli expression system. The protein is derived from the mouse/rat FGF-2 amino‑acid sequence (Ala11‑Ser154) and carries an N‑terminal 6×His tag to facilitate purification and detection.

Key Features

• High purity: >98% purity by SDS‑PAGE analysis
• Low endotoxin: LAL assay shows < 0.1 EU per µg protein
• High bioactivity: ED50 < 1.5 ng/mL, robustly induces 3T3 cell proliferation
• Animal‑origin‑free: No animal‑derived components added throughout production, reducing risk of exogenous contaminant exposure

Technical Specifications

• Species source: mouse/rat
• Expression host: Escherichia coli
• Protein length: 144 amino acids
• Molecular weight: 17.2 kDa (theoretical), migrates at ~19 kDa on SDS‑PAGE
• Tag: N‑terminal 6×His
• Form: lyophilized powder containing 0.01% sarkosyl in pH 8.0 PBS buffer

Bioactivity Validation

Determined by a 3T3 cell proliferation assay, the recombinant FGF‑2 exhibits excellent biological activity, with a specific activity exceeding 1×10⁶ IU/mg.

Recommended Usage

• Reconstitution: dissolve in sterile ddH₂O to a concentration ≥100 µg/mL, let stand at room temperature ≥20 min, avoid vortexing
• Storage: lyophilized powder stable at –20 °C for 1 year; after reconstitution, store at 2–8 °C for up to 1 week; aliquoted dilutions can be stored at –20 °C or –80 °C, avoid repeated freeze‑thaw cycles
• Shipping: transport on ice packs (blue ice)

Applications

• Stem and progenitor cell proliferation and differentiation studies
• Cell culture and organoid construction
• Tissue repair and wound‑healing mechanism investigations
• Tumor biology and angiogenesis research

Background

FGF‑2 (basic fibroblast growth factor, also known as HBGF‑2 or Prostatropin) is a pleiotropic cytokine that plays crucial roles in embryonic development, tissue repair, neurogenesis, and tumorigenesis. It is expressed in a wide range of cell types and holds extensive research and translational value.

Specifications and Pricing

Quantity	Price (CNY)	Lead Time
20 μg	588	In stock, ship next day
100 μg	1498	In stock, ship next day
500 μg	3998	Please inquire
1 mg	Please inquire	Please inquire

Note: This product is for research use only and is not intended for human or clinical diagnostic applications. Users are responsible for ensuring that its use does not infringe any patents or licensing requirements.

For additional information or custom requirements, please contact us at any time. Note: The above information may change due to product optimization or other factors; please consult Yakoin Co. for the latest details.