IL-17A 重组小鼠白介素17A 小鼠重组蛋白 细胞因子

重组小鼠白介素17A (IL-17A) 蛋白

英文名称: Mouse IL-17A Protein
货号: PRP1300

产品概述

本品为高纯度、无标签的重组小鼠白介素17A (IL-17A) 蛋白，专为细胞培养及相关生物医学研究设计。该蛋白由大肠杆菌表达系统制备，保证了其结构的准确性和批次间的高一致性。作为核心细胞因子产品，其在炎症机制、自身免疫性疾病及骨与软骨代谢研究领域具有重要应用价值。

产品特性

• 来源与序列: 氨基酸序列来源于小鼠源IL-17A (Thr22-Ala158) (UniProt ID: Q62386)。
• 种属: 小鼠
• 蛋白长度与分子量: 由137个氨基酸组成，预测分子量为15.4 KDa。
• 表达宿主: 大肠杆菌
• 纯度: > 98 % (SDS-PAGE检测)
• 产品形式: 冻干粉，冻干缓冲液为无菌的20 mM Tris，50 mM NaCl，pH 8.0。
• 内毒素水平: 通过LAL法测定，每微克蛋白内毒素含量 < 0.1 EU，有效降低由内毒素引起的非特异性实验干扰。
• 生物活性: 测试中 (Testing in Progress)。建议用户根据具体实验体系进行功能性验证。

产品优势

• 高纯度与低内毒素: 严格的质控流程确保了产品的高纯度与极低的内毒素水平，为细胞实验提供可靠保障。
• 无标签设计: 蛋白不含任何附加标签，尽可能减少了标签对蛋白天然功能及下游应用可能产生的影响。
• 即用型规格: 提供多种规格选择，满足从初步筛选到大规模实验的不同需求，且常规规格均为现货，发货迅速。

使用说明

复溶建议: 开盖前请先离心。推荐使用产品配套的复溶缓冲液进行复溶，初始复溶浓度建议不低于104 µg/mL。用户可根据后续实验需求进行进一步稀释。

保存条件: 冻干粉应保存于-20℃以下的干燥环境中。复溶后的蛋白可在4℃下短期保存（2-7天），长期保存请置于-20℃以下。为保持长期稳定性，建议添加载体蛋白（如0.1% HSA或BSA）并分装保存，避免反复冻融。

运输条件: 冰袋运输（蓝冰）。

背景信息

白介素17 (IL-17) 是一种由活化记忆T细胞产生的强效促炎细胞因子。成熟的小鼠IL-17A与人和大鼠的IL-17A分别具有61%和89%的氨基酸序列同源性。IL-17具有与IL-1和TNF-α相似的特性，可能诱导关节炎症以及骨和软骨的破坏。该细胞因子在类风湿关节炎患者的滑液中被发现，并参与增加IL-6产生、诱导胶原降解、抑制胶原和蛋白聚糖合成等过程，在骨破坏与形成调节中扮演关键角色。

规格与价格

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	4398	现货（次日发货）
1 mg	请咨询	请咨询

警告: 本品仅供研究使用，不适用于人类或临床诊断。使用本产品可能受相关专利或许可证限制，用户应自行负责确保其使用符合所有适用的法律法规。

基因ID: 10220 | 蛋白质ID: Q62386 | 别名: interleukin 17A, CTLA-8, IL17, IL17A

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Mouse Interleukin‑17A (IL‑17A) Protein

English Name: Mouse IL‑17A Protein
Product No.: PRP1300

Product Overview

This product is a high‑purity, tag‑free recombinant mouse interleukin‑17A (IL‑17A) protein, specifically designed for cell‑culture and related biomedical research. The protein is produced using an Escherichia coli expression system, ensuring accurate structure and high batch‑to‑batch consistency. As a core cytokine reagent, it has important applications in studies of inflammatory mechanisms, autoimmune diseases, and bone and cartilage metabolism.

Product Features

• Source and Sequence: Amino‑acid sequence derived from mouse IL‑17A (Thr22‑Ala158) (UniProt ID: Q62386).
• Species: Mouse
• Protein Length and Molecular Weight: 137 amino acids; predicted molecular weight 15.4 kDa.
• Expression Host: E. coli
• Purity: > 98 % (SDS‑PAGE)
• Product Form: Lyophilized powder; lyophilization buffer is sterile 20 mM Tris, 50 mM NaCl, pH 8.0.
• Endotoxin Level: Determined by LAL assay; endotoxin < 0.1 EU per µg protein, effectively reducing nonspecific assay interference caused by endotoxin.
• Biological Activity: Testing in Progress. Users are advised to validate functionality according to their specific assay systems.

Product Advantages

• High Purity and Low Endotoxin: Strict QC processes ensure high purity and ultra‑low endotoxin levels, providing reliable performance in cell‑based assays.
• Tag‑Free Design: The protein contains no additional tags, minimizing potential impacts on native activity and downstream applications.
• Ready‑to‑Use Formats: Multiple size options are available to meet needs from preliminary screening to large‑scale studies; standard formats are stocked for rapid shipment.

Usage Instructions

Reconstitution Recommendation: Centrifuge the vial before opening. Reconstitute with the supplied buffer; initial concentration should be ≥ 104 µg/mL. Further dilutions can be performed as required by downstream assays.

Storage Conditions: Store lyophilized powder in a dry environment at ≤ ‑20 °C. Reconstituted protein can be kept short‑term at 4 °C (2–7 days); for long‑term storage keep at ≤ ‑20 °C. To maintain stability, add a carrier protein (e.g., 0.1 % HSA or BSA) and aliquot to avoid repeated freeze‑thaw cycles.

Shipping Conditions: Shipped on ice packs (blue ice).

Background Information

Interleukin‑17 (IL‑17) is a potent pro‑inflammatory cytokine produced by activated memory T cells. Mature mouse IL‑17A shares 61 % and 89 % amino‑acid sequence homology with human and rat IL‑17A, respectively. IL‑17 exhibits properties similar to IL‑1 and TNF‑α and may induce joint inflammation as well as bone and cartilage degradation. This cytokine has been detected in the synovial fluid of rheumatoid arthritis patients and participates in up‑regulating IL‑6 production, inducing collagen breakdown, and suppressing collagen and proteoglycan synthesis, playing a pivotal role in the regulation of bone resorption and formation.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock (next‑day shipment)
20 µg	1498	In stock (next‑day shipment)
100 µg	4398	In stock (next‑day shipment)
1 mg	Please inquire	Please inquire

Warning: This product is for research use only and is not intended for human or clinical diagnostic applications. Use may be subject to relevant patents or licenses; users are responsible for ensuring compliance with all applicable laws and regulations.

Gene ID: 10220 | Protein ID: Q62386 | Synonyms: interleukin 17A, CTLA‑8, IL‑17, IL‑17A

Note: The above information may change due to product optimization or upgrades; for the latest details, please contact Yakolin Company.